Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art
In the past decade, adipose tissue became a highly interesting source of adult stem cells for plastic surgery and regenerative medicine. The isolated stromal vascular fraction (SVF) is a heterogeneous cell population including the adipose-derived stromal/stem cells (ASC), which showed regenerative p...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2015-01-01
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| Series: | Cell Regeneration |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2045976917300093 |
| _version_ | 1811333463125524480 |
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| author | Eleni Oberbauer Carolin Steffenhagen Christoph Wurzer Christian Gabriel Heinz Redl Susanne Wolbank |
| author_facet | Eleni Oberbauer Carolin Steffenhagen Christoph Wurzer Christian Gabriel Heinz Redl Susanne Wolbank |
| author_sort | Eleni Oberbauer |
| collection | DOAJ |
| description | In the past decade, adipose tissue became a highly interesting source of adult stem cells for plastic surgery and regenerative medicine. The isolated stromal vascular fraction (SVF) is a heterogeneous cell population including the adipose-derived stromal/stem cells (ASC), which showed regenerative potential in several clinical studies and trials. SVF should be provided in a safe and reproducible manner in accordance with current good manufacturing practices (cGMP). To ensure highest possible safety for patients, a precisely defined procedure with a high-quality control is required. Hence, an increasing number of adipose tissue-derived cell isolation systems have been developed. These systems aim for a closed, sterile, and safe isolation process limiting donor variations, risk for contaminations, and unpredictability of the cell material. To isolate SVF from adipose tissue, enzymes such as collagenase are used. Alternatively, in order to avoid enzymes, isolation systems using physical forces are available. Here, we provide an overview of known existing enzymatic and non-enzymatic adipose tissue-derived cell isolation systems, which are patented, published, or already on the market. |
| first_indexed | 2024-04-13T16:53:14Z |
| format | Article |
| id | doaj.art-a71c19780fa8474ba5efdd88da2fe0a5 |
| institution | Directory Open Access Journal |
| issn | 2045-9769 |
| language | English |
| last_indexed | 2024-04-13T16:53:14Z |
| publishDate | 2015-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Cell Regeneration |
| spelling | doaj.art-a71c19780fa8474ba5efdd88da2fe0a52022-12-22T02:38:54ZengSpringerOpenCell Regeneration2045-97692015-01-014110.1186/s13619-015-0020-0Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the artEleni Oberbauer0Carolin Steffenhagen1Christoph Wurzer2Christian Gabriel3Heinz Redl4Susanne Wolbank5Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Linz/Vienna, AustriaLudwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Linz/Vienna, AustriaLudwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Linz/Vienna, AustriaLudwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Linz/Vienna, AustriaLudwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Linz/Vienna, AustriaLudwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, Linz/Vienna, AustriaIn the past decade, adipose tissue became a highly interesting source of adult stem cells for plastic surgery and regenerative medicine. The isolated stromal vascular fraction (SVF) is a heterogeneous cell population including the adipose-derived stromal/stem cells (ASC), which showed regenerative potential in several clinical studies and trials. SVF should be provided in a safe and reproducible manner in accordance with current good manufacturing practices (cGMP). To ensure highest possible safety for patients, a precisely defined procedure with a high-quality control is required. Hence, an increasing number of adipose tissue-derived cell isolation systems have been developed. These systems aim for a closed, sterile, and safe isolation process limiting donor variations, risk for contaminations, and unpredictability of the cell material. To isolate SVF from adipose tissue, enzymes such as collagenase are used. Alternatively, in order to avoid enzymes, isolation systems using physical forces are available. Here, we provide an overview of known existing enzymatic and non-enzymatic adipose tissue-derived cell isolation systems, which are patented, published, or already on the market.http://www.sciencedirect.com/science/article/pii/S2045976917300093Human adipose tissueStromal vascular fractionAdipose-derived stromal/stem cellsEnzymaticNon-enzymaticIsolation systems |
| spellingShingle | Eleni Oberbauer Carolin Steffenhagen Christoph Wurzer Christian Gabriel Heinz Redl Susanne Wolbank Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art Cell Regeneration Human adipose tissue Stromal vascular fraction Adipose-derived stromal/stem cells Enzymatic Non-enzymatic Isolation systems |
| title | Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art |
| title_full | Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art |
| title_fullStr | Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art |
| title_full_unstemmed | Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art |
| title_short | Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art |
| title_sort | enzymatic and non enzymatic isolation systems for adipose tissue derived cells current state of the art |
| topic | Human adipose tissue Stromal vascular fraction Adipose-derived stromal/stem cells Enzymatic Non-enzymatic Isolation systems |
| url | http://www.sciencedirect.com/science/article/pii/S2045976917300093 |
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