Nanoscale and functional heterogeneity of the hippocampal extracellular space
Summary: The extracellular space (ECS) and its constituents play a crucial role in brain development, plasticity, circadian rhythm, and behavior, as well as brain diseases. Yet, since this compartment has an intricate geometry and nanoscale dimensions, its detailed exploration in live tissue has rem...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723004898 |
| _version_ | 1797832308218134528 |
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| author | Diego Grassi Agata Idziak Antony Lee Ivo Calaresu Jean-Baptiste Sibarita Laurent Cognet U. Valentin Nägerl Laurent Groc |
| author_facet | Diego Grassi Agata Idziak Antony Lee Ivo Calaresu Jean-Baptiste Sibarita Laurent Cognet U. Valentin Nägerl Laurent Groc |
| author_sort | Diego Grassi |
| collection | DOAJ |
| description | Summary: The extracellular space (ECS) and its constituents play a crucial role in brain development, plasticity, circadian rhythm, and behavior, as well as brain diseases. Yet, since this compartment has an intricate geometry and nanoscale dimensions, its detailed exploration in live tissue has remained an unmet challenge. Here, we used a combination of single-nanoparticle tracking and super-resolution microscopy approaches to map the nanoscale dimensions of the ECS across the rodent hippocampus. We report that these dimensions are heterogeneous between hippocampal areas. Notably, stratum radiatum CA1 and CA3 ECS differ in several characteristics, a difference that gets abolished after digestion of the extracellular matrix. The dynamics of extracellular immunoglobulins vary within these areas, consistent with their distinct ECS characteristics. Altogether, we demonstrate that ECS nanoscale anatomy and diffusion properties are widely heterogeneous across hippocampal areas, impacting the dynamics and distribution of extracellular molecules. |
| first_indexed | 2024-04-09T14:06:50Z |
| format | Article |
| id | doaj.art-a71c2a25120442fd9c5a13de20c4ab19 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-09T14:06:50Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-a71c2a25120442fd9c5a13de20c4ab192023-05-07T04:16:42ZengElsevierCell Reports2211-12472023-05-01425112478Nanoscale and functional heterogeneity of the hippocampal extracellular spaceDiego Grassi0Agata Idziak1Antony Lee2Ivo Calaresu3Jean-Baptiste Sibarita4Laurent Cognet5U. Valentin Nägerl6Laurent Groc7University of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, 33000 Bordeaux, FranceUniversity of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, 33000 Bordeaux, FranceUniversity of Bordeaux, Laboratoire Photonique Numérique et Nanosciences (LP2N), UMR 5298, 33400 Talence, France; Institut d’Optique & CNRS, LP2N UMR 5298, 33400 Talence, FranceUniversity of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, 33000 Bordeaux, FranceUniversity of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, 33000 Bordeaux, FranceUniversity of Bordeaux, Laboratoire Photonique Numérique et Nanosciences (LP2N), UMR 5298, 33400 Talence, France; Institut d’Optique & CNRS, LP2N UMR 5298, 33400 Talence, FranceUniversity of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, 33000 Bordeaux, FranceUniversity of Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, 33000 Bordeaux, France; Corresponding authorSummary: The extracellular space (ECS) and its constituents play a crucial role in brain development, plasticity, circadian rhythm, and behavior, as well as brain diseases. Yet, since this compartment has an intricate geometry and nanoscale dimensions, its detailed exploration in live tissue has remained an unmet challenge. Here, we used a combination of single-nanoparticle tracking and super-resolution microscopy approaches to map the nanoscale dimensions of the ECS across the rodent hippocampus. We report that these dimensions are heterogeneous between hippocampal areas. Notably, stratum radiatum CA1 and CA3 ECS differ in several characteristics, a difference that gets abolished after digestion of the extracellular matrix. The dynamics of extracellular immunoglobulins vary within these areas, consistent with their distinct ECS characteristics. Altogether, we demonstrate that ECS nanoscale anatomy and diffusion properties are widely heterogeneous across hippocampal areas, impacting the dynamics and distribution of extracellular molecules.http://www.sciencedirect.com/science/article/pii/S2211124723004898CP: Neuroscience |
| spellingShingle | Diego Grassi Agata Idziak Antony Lee Ivo Calaresu Jean-Baptiste Sibarita Laurent Cognet U. Valentin Nägerl Laurent Groc Nanoscale and functional heterogeneity of the hippocampal extracellular space Cell Reports CP: Neuroscience |
| title | Nanoscale and functional heterogeneity of the hippocampal extracellular space |
| title_full | Nanoscale and functional heterogeneity of the hippocampal extracellular space |
| title_fullStr | Nanoscale and functional heterogeneity of the hippocampal extracellular space |
| title_full_unstemmed | Nanoscale and functional heterogeneity of the hippocampal extracellular space |
| title_short | Nanoscale and functional heterogeneity of the hippocampal extracellular space |
| title_sort | nanoscale and functional heterogeneity of the hippocampal extracellular space |
| topic | CP: Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723004898 |
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