Circulating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of diabetic cystopathy
Abstract Diabetic cystopathy (DCP) is a prevalent etiology of bladder dysfunction in individuals with longstanding diabetes, frequently leading to bladder interstitial fibrosis. Research investigating the initial pathological alterations of DCP is notably scarce. To comprehend the development of fib...
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| Format: | Article |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-51451-7 |
| _version_ | 1797355873942634496 |
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| author | Bo Xue Gaohaer Kadeerhan Li-bin Sun Yong-quan Chen Xiao-feng Hu Zi-kuan Zhang Dong-wen Wang |
| author_facet | Bo Xue Gaohaer Kadeerhan Li-bin Sun Yong-quan Chen Xiao-feng Hu Zi-kuan Zhang Dong-wen Wang |
| author_sort | Bo Xue |
| collection | DOAJ |
| description | Abstract Diabetic cystopathy (DCP) is a prevalent etiology of bladder dysfunction in individuals with longstanding diabetes, frequently leading to bladder interstitial fibrosis. Research investigating the initial pathological alterations of DCP is notably scarce. To comprehend the development of fibrosis and find effective biomarkers for its diagnosis, we prepared streptozotocin-induced long-term diabetic SD rats exhibiting a type 1 diabetes phenotype and bladder fibrosis in histology detection. After observing myofibroblast differentiation from rats’ primary bladder fibroblasts with immunofluorescence, we isolated fibroblasts derived exosomes and performed exosomal miRNA sequencing. The co-differentially expressed miRNAs (DEMis) (miR-16-5p and let-7e-5p) were screened through a joint analysis of diabetic rats and long-term patients’ plasma data (GES97123) downloaded from the GEO database. Then two co-DEMis were validated by quantitative PCR on exosomes derived from diabetic rats’ plasma. Following with a series of analysis, including target mRNAs and transcription factors (TFs) prediction, hubgenes identification, protein–protein interaction (PPI) network construction and gene enrichment analysis, a miRNA-mediated genetic regulatory network consisting of two miRNAs, nine TFs, and thirty target mRNAs were identified in relation to fibrotic processes. Thus, circulating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of DCP, and the crucial genes in regulatory network might hold immense significance in studying the pathogenesis and molecular mechanisms of fibrosis, which deserves further exploration. |
| first_indexed | 2024-03-08T14:17:26Z |
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| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T14:17:26Z |
| publishDate | 2024-01-01 |
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| series | Scientific Reports |
| spelling | doaj.art-a71d93d0816d4e5ca6c09ec826cc4c202024-01-14T12:20:11ZengNature PortfolioScientific Reports2045-23222024-01-0114111610.1038/s41598-024-51451-7Circulating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of diabetic cystopathyBo Xue0Gaohaer Kadeerhan1Li-bin Sun2Yong-quan Chen3Xiao-feng Hu4Zi-kuan Zhang5Dong-wen Wang6Shanxi Medical UniversityDepartment of Urology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical CollegeShanxi Medical UniversityShanxi Medical UniversityShanxi Medical UniversityShanxi Medical UniversityShanxi Medical UniversityAbstract Diabetic cystopathy (DCP) is a prevalent etiology of bladder dysfunction in individuals with longstanding diabetes, frequently leading to bladder interstitial fibrosis. Research investigating the initial pathological alterations of DCP is notably scarce. To comprehend the development of fibrosis and find effective biomarkers for its diagnosis, we prepared streptozotocin-induced long-term diabetic SD rats exhibiting a type 1 diabetes phenotype and bladder fibrosis in histology detection. After observing myofibroblast differentiation from rats’ primary bladder fibroblasts with immunofluorescence, we isolated fibroblasts derived exosomes and performed exosomal miRNA sequencing. The co-differentially expressed miRNAs (DEMis) (miR-16-5p and let-7e-5p) were screened through a joint analysis of diabetic rats and long-term patients’ plasma data (GES97123) downloaded from the GEO database. Then two co-DEMis were validated by quantitative PCR on exosomes derived from diabetic rats’ plasma. Following with a series of analysis, including target mRNAs and transcription factors (TFs) prediction, hubgenes identification, protein–protein interaction (PPI) network construction and gene enrichment analysis, a miRNA-mediated genetic regulatory network consisting of two miRNAs, nine TFs, and thirty target mRNAs were identified in relation to fibrotic processes. Thus, circulating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of DCP, and the crucial genes in regulatory network might hold immense significance in studying the pathogenesis and molecular mechanisms of fibrosis, which deserves further exploration.https://doi.org/10.1038/s41598-024-51451-7 |
| spellingShingle | Bo Xue Gaohaer Kadeerhan Li-bin Sun Yong-quan Chen Xiao-feng Hu Zi-kuan Zhang Dong-wen Wang Circulating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of diabetic cystopathy Scientific Reports |
| title | Circulating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of diabetic cystopathy |
| title_full | Circulating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of diabetic cystopathy |
| title_fullStr | Circulating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of diabetic cystopathy |
| title_full_unstemmed | Circulating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of diabetic cystopathy |
| title_short | Circulating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of diabetic cystopathy |
| title_sort | circulating exosomal mir 16 5p and let 7e 5p are associated with bladder fibrosis of diabetic cystopathy |
| url | https://doi.org/10.1038/s41598-024-51451-7 |
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