The Effects of Levels of Oral Di-(2-ethylhexyl) Phthalate on Glycemic Response in Mice
Background & Objective: Humans are widely exposed to di-(2-ethylhexyl) phthalate that it can rise obesity and type 2 diabetes epidemics. The object of this study was to investigate the effects of oral di-(2-ethylhexyl) phthalate (DEHP) at minimum and maximum levels on glycemic response in mice....
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fasa University of Medical Sciences
2018-04-01
|
| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-1175-en.pdf |
| _version_ | 1827812381995040768 |
|---|---|
| author | Behzad Najmi kargan Ali Kargari Rezapour Rasoul Sharifi |
| author_facet | Behzad Najmi kargan Ali Kargari Rezapour Rasoul Sharifi |
| author_sort | Behzad Najmi kargan |
| collection | DOAJ |
| description | Background & Objective: Humans are widely exposed to di-(2-ethylhexyl) phthalate that it can rise obesity and type 2 diabetes epidemics. The object of this study was to investigate the effects of oral di-(2-ethylhexyl) phthalate (DEHP) at minimum and maximum levels on glycemic response in mice.
Material & Methods: An interventional study with 15 adult male mice was designed. Mice were randomly assigned to 3 equal groups: control, 1x phthalate (dose of 250 mg/kg/day), 2x phthalate (dose of 500 mg/kg/day) and treated for a period of 8 weeks. At the end of the seventh week study all mice were tested by oral glucose tolerance test and area under curve (AUC) was calculated for each sample. In the end, fasting blood samples were used to measure the variables (Glucose, Insulin, HOMA_IR, Quicki of serum and HbA1C of total blood). The data were analyzed by ANOVA and Duncan tests. The significant difference in the data was considered P >0.01.
Results: Di-(2-ethylhexyl) phthalate at low dose (250 mg/kg) showed significant increase in serum glucose and insulin resistance levels and also quicki levels decreased significantly compared to other groups (P <0.01). This study showed no significant changes in insulin serum, glycated hemoglobin (HbA1c) of total blood and area under the curve in glucose tolerance test (P >0.01).
Conclusion: In the present study, it showed di-(2-ethylhexyl) phthalate at low dose (250 mg/kg) impaired blood sugar control systems and this can lead in pre-diabetes. |
| first_indexed | 2024-03-11T23:17:48Z |
| format | Article |
| id | doaj.art-a71da34dbd994182ab913246f3fdc4d5 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:17:48Z |
| publishDate | 2018-04-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-a71da34dbd994182ab913246f3fdc4d52023-09-20T21:23:48ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232018-04-0181601607The Effects of Levels of Oral Di-(2-ethylhexyl) Phthalate on Glycemic Response in MiceBehzad Najmi kargan0Ali Kargari Rezapour1Rasoul Sharifi2 Department of Biologhy, Faculty of Basic Sciences, Islamic Azad University of Ahar, Ahar, Iran Department of Clinical pathology, Faculty of Veterinary Medicine, Islamic Azad University of Tabriz, Tabriz, Iran Department of Biologhy, Faculty of Basic Sciences, Islamic Azad University of Ahar, Ahar, Iran Background & Objective: Humans are widely exposed to di-(2-ethylhexyl) phthalate that it can rise obesity and type 2 diabetes epidemics. The object of this study was to investigate the effects of oral di-(2-ethylhexyl) phthalate (DEHP) at minimum and maximum levels on glycemic response in mice. Material & Methods: An interventional study with 15 adult male mice was designed. Mice were randomly assigned to 3 equal groups: control, 1x phthalate (dose of 250 mg/kg/day), 2x phthalate (dose of 500 mg/kg/day) and treated for a period of 8 weeks. At the end of the seventh week study all mice were tested by oral glucose tolerance test and area under curve (AUC) was calculated for each sample. In the end, fasting blood samples were used to measure the variables (Glucose, Insulin, HOMA_IR, Quicki of serum and HbA1C of total blood). The data were analyzed by ANOVA and Duncan tests. The significant difference in the data was considered P >0.01. Results: Di-(2-ethylhexyl) phthalate at low dose (250 mg/kg) showed significant increase in serum glucose and insulin resistance levels and also quicki levels decreased significantly compared to other groups (P <0.01). This study showed no significant changes in insulin serum, glycated hemoglobin (HbA1c) of total blood and area under the curve in glucose tolerance test (P >0.01). Conclusion: In the present study, it showed di-(2-ethylhexyl) phthalate at low dose (250 mg/kg) impaired blood sugar control systems and this can lead in pre-diabetes.http://jabs.fums.ac.ir/article-1-1175-en.pdfdi-(2-ethylhexyl) phthalateglycemic responsemice |
| spellingShingle | Behzad Najmi kargan Ali Kargari Rezapour Rasoul Sharifi The Effects of Levels of Oral Di-(2-ethylhexyl) Phthalate on Glycemic Response in Mice Journal of Advanced Biomedical Sciences di-(2-ethylhexyl) phthalate glycemic response mice |
| title | The Effects of Levels of Oral Di-(2-ethylhexyl) Phthalate on Glycemic Response in Mice |
| title_full | The Effects of Levels of Oral Di-(2-ethylhexyl) Phthalate on Glycemic Response in Mice |
| title_fullStr | The Effects of Levels of Oral Di-(2-ethylhexyl) Phthalate on Glycemic Response in Mice |
| title_full_unstemmed | The Effects of Levels of Oral Di-(2-ethylhexyl) Phthalate on Glycemic Response in Mice |
| title_short | The Effects of Levels of Oral Di-(2-ethylhexyl) Phthalate on Glycemic Response in Mice |
| title_sort | effects of levels of oral di 2 ethylhexyl phthalate on glycemic response in mice |
| topic | di-(2-ethylhexyl) phthalate glycemic response mice |
| url | http://jabs.fums.ac.ir/article-1-1175-en.pdf |
| work_keys_str_mv | AT behzadnajmikargan theeffectsoflevelsoforaldi2ethylhexylphthalateonglycemicresponseinmice AT alikargarirezapour theeffectsoflevelsoforaldi2ethylhexylphthalateonglycemicresponseinmice AT rasoulsharifi theeffectsoflevelsoforaldi2ethylhexylphthalateonglycemicresponseinmice AT behzadnajmikargan effectsoflevelsoforaldi2ethylhexylphthalateonglycemicresponseinmice AT alikargarirezapour effectsoflevelsoforaldi2ethylhexylphthalateonglycemicresponseinmice AT rasoulsharifi effectsoflevelsoforaldi2ethylhexylphthalateonglycemicresponseinmice |