Assessment of lipid composition and eicosapentaenoic acid/docosahexaenoic acid bioavailability in fish oil obtained through different enrichment methods
In this study, we analyzed the eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA) lipid composition of fish oil obtained through enzymatic treatment, fractional distillation and silica gel column purification, and further assessed EPA/DHA bioavailability. Lipid subclass composition information was...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1136490/full |
| _version_ | 1797871446379200512 |
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| author | Rongzhen Song Wen Li Shanggui Deng Yueliang Zhao Ningping Tao Ningping Tao Ningping Tao |
| author_facet | Rongzhen Song Wen Li Shanggui Deng Yueliang Zhao Ningping Tao Ningping Tao Ningping Tao |
| author_sort | Rongzhen Song |
| collection | DOAJ |
| description | In this study, we analyzed the eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA) lipid composition of fish oil obtained through enzymatic treatment, fractional distillation and silica gel column purification, and further assessed EPA/DHA bioavailability. Lipid subclass composition information was obtained through ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS), and bioavailability tests were performed using the Caco-2 cell monolayer model. Results showed that enzymatic treatment improved the incorporation of EPA/DHA as diacylglycerol (DG) while silica gel column chromatography enriched the content of EPA/DHA as phosphatidylglycerol (PG) (12.58%) and phosphatidylethanolamine (PE) (4.99%). Furthermore, increasing the purity of EPA/DHA could improve its bioavailability and after 24 incubation, binding forms of triglyceride (TG) was superior to ethyl ester (EE) (p < 0.05) at the same purity level. Those findings are helpful to provide research basis for exploring the bioactivity of fish oil. |
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| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-10T00:43:11Z |
| publishDate | 2023-03-01 |
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| series | Frontiers in Nutrition |
| spelling | doaj.art-a72015e5db624b01af506d64d92cd4fd2023-03-14T04:49:04ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-03-011010.3389/fnut.2023.11364901136490Assessment of lipid composition and eicosapentaenoic acid/docosahexaenoic acid bioavailability in fish oil obtained through different enrichment methodsRongzhen Song0Wen Li1Shanggui Deng2Yueliang Zhao3Ningping Tao4Ningping Tao5Ningping Tao6College of Food Science and Technology, Shanghai Ocean University, Shanghai, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, ChinaCollege of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, ChinaShanghai Engineering Research Center of Aquatic-Product Processing and Preservation, Shanghai, ChinaNational Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai, ChinaIn this study, we analyzed the eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA) lipid composition of fish oil obtained through enzymatic treatment, fractional distillation and silica gel column purification, and further assessed EPA/DHA bioavailability. Lipid subclass composition information was obtained through ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS), and bioavailability tests were performed using the Caco-2 cell monolayer model. Results showed that enzymatic treatment improved the incorporation of EPA/DHA as diacylglycerol (DG) while silica gel column chromatography enriched the content of EPA/DHA as phosphatidylglycerol (PG) (12.58%) and phosphatidylethanolamine (PE) (4.99%). Furthermore, increasing the purity of EPA/DHA could improve its bioavailability and after 24 incubation, binding forms of triglyceride (TG) was superior to ethyl ester (EE) (p < 0.05) at the same purity level. Those findings are helpful to provide research basis for exploring the bioactivity of fish oil.https://www.frontiersin.org/articles/10.3389/fnut.2023.1136490/fulleicosapentaenoic acid (EPA)docosahexaenoic acid (DHA)lipids compositionCaco-2 cellbioavailability |
| spellingShingle | Rongzhen Song Wen Li Shanggui Deng Yueliang Zhao Ningping Tao Ningping Tao Ningping Tao Assessment of lipid composition and eicosapentaenoic acid/docosahexaenoic acid bioavailability in fish oil obtained through different enrichment methods Frontiers in Nutrition eicosapentaenoic acid (EPA) docosahexaenoic acid (DHA) lipids composition Caco-2 cell bioavailability |
| title | Assessment of lipid composition and eicosapentaenoic acid/docosahexaenoic acid bioavailability in fish oil obtained through different enrichment methods |
| title_full | Assessment of lipid composition and eicosapentaenoic acid/docosahexaenoic acid bioavailability in fish oil obtained through different enrichment methods |
| title_fullStr | Assessment of lipid composition and eicosapentaenoic acid/docosahexaenoic acid bioavailability in fish oil obtained through different enrichment methods |
| title_full_unstemmed | Assessment of lipid composition and eicosapentaenoic acid/docosahexaenoic acid bioavailability in fish oil obtained through different enrichment methods |
| title_short | Assessment of lipid composition and eicosapentaenoic acid/docosahexaenoic acid bioavailability in fish oil obtained through different enrichment methods |
| title_sort | assessment of lipid composition and eicosapentaenoic acid docosahexaenoic acid bioavailability in fish oil obtained through different enrichment methods |
| topic | eicosapentaenoic acid (EPA) docosahexaenoic acid (DHA) lipids composition Caco-2 cell bioavailability |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1136490/full |
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