A Lateral Flow Assay for the Detection of <i>Leptospira lipL32</i> Gene Using CRISPR Technology
The clinical manifestation of leptospirosis is often misdiagnosed as other febrile illnesses such as dengue. Therefore, there is an urgent need for a precise diagnostic tool at the field level to detect the pathogenic <i>Leptospira lipL32</i> gene at the molecular level for prompt therap...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/14/6544 |
| _version_ | 1797587538000478208 |
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| author | Satheesh Natarajan Jayaraj Joseph Balamurugan Vinayagamurthy Pedro Estrela |
| author_facet | Satheesh Natarajan Jayaraj Joseph Balamurugan Vinayagamurthy Pedro Estrela |
| author_sort | Satheesh Natarajan |
| collection | DOAJ |
| description | The clinical manifestation of leptospirosis is often misdiagnosed as other febrile illnesses such as dengue. Therefore, there is an urgent need for a precise diagnostic tool at the field level to detect the pathogenic <i>Leptospira lipL32</i> gene at the molecular level for prompt therapeutic decisions. Quantitative polymerase chain reaction (qPCR) is widely used as the primary diagnostic tool, but its applicability is limited by high equipment cost and the lack of availability in every hospital, especially in rural areas where leptospirosis mainly occurs. Here, we report the development of a CRISPR dFnCas9-based quantitative lateral flow immunoassay to detect the <i>lipL32</i> gene. The developed assay showed superior performance regarding the lowest detectable limit of 1 fg/mL. The test is highly sensitive and selective, showing that leptospirosis diagnosis can be achieved with a low-cost lateral flow device. |
| first_indexed | 2024-03-11T00:40:17Z |
| format | Article |
| id | doaj.art-a720c7c4c0274b158faea6049bb73eb4 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-11T00:40:17Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-a720c7c4c0274b158faea6049bb73eb42023-11-18T21:19:12ZengMDPI AGSensors1424-82202023-07-012314654410.3390/s23146544A Lateral Flow Assay for the Detection of <i>Leptospira lipL32</i> Gene Using CRISPR TechnologySatheesh Natarajan0Jayaraj Joseph1Balamurugan Vinayagamurthy2Pedro Estrela3Healthcare Technology Innovation Centre, Indian Institute of Technology Madras, Chennai 600113, IndiaDepartment of Electrical Engineering, Indian Institute of Technology Madras, Chennai 600036, IndiaIndian Council of Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics (ICAR-NIVEDI), Bangalore 560064, IndiaDepartment of Electronic and Electrical Engineering, University of Bath, Bath BA2 7AY, UKThe clinical manifestation of leptospirosis is often misdiagnosed as other febrile illnesses such as dengue. Therefore, there is an urgent need for a precise diagnostic tool at the field level to detect the pathogenic <i>Leptospira lipL32</i> gene at the molecular level for prompt therapeutic decisions. Quantitative polymerase chain reaction (qPCR) is widely used as the primary diagnostic tool, but its applicability is limited by high equipment cost and the lack of availability in every hospital, especially in rural areas where leptospirosis mainly occurs. Here, we report the development of a CRISPR dFnCas9-based quantitative lateral flow immunoassay to detect the <i>lipL32</i> gene. The developed assay showed superior performance regarding the lowest detectable limit of 1 fg/mL. The test is highly sensitive and selective, showing that leptospirosis diagnosis can be achieved with a low-cost lateral flow device.https://www.mdpi.com/1424-8220/23/14/6544<i>Leptospira</i>CRISPRdFnCas9lateral flow device |
| spellingShingle | Satheesh Natarajan Jayaraj Joseph Balamurugan Vinayagamurthy Pedro Estrela A Lateral Flow Assay for the Detection of <i>Leptospira lipL32</i> Gene Using CRISPR Technology Sensors <i>Leptospira</i> CRISPR dFnCas9 lateral flow device |
| title | A Lateral Flow Assay for the Detection of <i>Leptospira lipL32</i> Gene Using CRISPR Technology |
| title_full | A Lateral Flow Assay for the Detection of <i>Leptospira lipL32</i> Gene Using CRISPR Technology |
| title_fullStr | A Lateral Flow Assay for the Detection of <i>Leptospira lipL32</i> Gene Using CRISPR Technology |
| title_full_unstemmed | A Lateral Flow Assay for the Detection of <i>Leptospira lipL32</i> Gene Using CRISPR Technology |
| title_short | A Lateral Flow Assay for the Detection of <i>Leptospira lipL32</i> Gene Using CRISPR Technology |
| title_sort | lateral flow assay for the detection of i leptospira lipl32 i gene using crispr technology |
| topic | <i>Leptospira</i> CRISPR dFnCas9 lateral flow device |
| url | https://www.mdpi.com/1424-8220/23/14/6544 |
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