Comprehensive Evaluation of Probiotic Property, Hypoglycemic Ability and Antioxidant Activity of Lactic Acid Bacteria
Taking lactic acid bacteria is an important strategy to alleviate or prevent diabetes, but the candidate strains with good genetic stability and excellent functions still need to be supplemented. In this study, the hypoglycemic ability (α-amylase, α-glucosidase and dipeptidyl peptidase 4), probiotic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/9/1363 |
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| author | Hongyu Wang Liang Li |
| author_facet | Hongyu Wang Liang Li |
| author_sort | Hongyu Wang |
| collection | DOAJ |
| description | Taking lactic acid bacteria is an important strategy to alleviate or prevent diabetes, but the candidate strains with good genetic stability and excellent functions still need to be supplemented. In this study, the hypoglycemic ability (α-amylase, α-glucosidase and dipeptidyl peptidase 4), probiotic property and antioxidant activity of lactic acid bacteria were comprehensively evaluated by a principal component analysis (PCA) and analytic hierarchy process (AHP). The results showed that <i>L</i><i>actobacillus paracasei</i><i>(L. paracasei</i>) had a higher survival rate (82.78%) in gastric juice and good tolerance to bile salt, and can be colonized in HT-29 cells. <i>L. paracasei</i> had a remarkable inhibitive activity of α-amylase (82.21%), α-glucosidase (84.29%) and dipeptidyl peptidase 4 (42.51%). <i>L. paracasei</i> had better scavenging activity of free radicals, total antioxidant activity (FRAP) and superoxide dismutase activity. According to the scores of the PCA, <i>L. paracasei</i> had the best hypoglycemic ability, and <i>L</i><i>actococcus lactis</i> (<i>L. lactis</i>) had the highest probiotic property. According to AHP, <i>L. paracasei</i> was the best potential hypoglycemic probiotic; furthermore, <i>L. lactis</i> showed the highest comprehensive performance except <i>Lactobacillus</i>. All lactic acid bacteria in this test had good safety. <i>L. paracasei</i> is expected to become a new potential hypoglycemic strain. |
| first_indexed | 2024-03-10T04:09:26Z |
| format | Article |
| id | doaj.art-a724f6671ae04b06a7aa9b3dc6146ade |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T04:09:26Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-a724f6671ae04b06a7aa9b3dc6146ade2023-11-23T08:14:25ZengMDPI AGFoods2304-81582022-05-01119136310.3390/foods11091363Comprehensive Evaluation of Probiotic Property, Hypoglycemic Ability and Antioxidant Activity of Lactic Acid BacteriaHongyu Wang0Liang Li1State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, ChinaState Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, ChinaTaking lactic acid bacteria is an important strategy to alleviate or prevent diabetes, but the candidate strains with good genetic stability and excellent functions still need to be supplemented. In this study, the hypoglycemic ability (α-amylase, α-glucosidase and dipeptidyl peptidase 4), probiotic property and antioxidant activity of lactic acid bacteria were comprehensively evaluated by a principal component analysis (PCA) and analytic hierarchy process (AHP). The results showed that <i>L</i><i>actobacillus paracasei</i><i>(L. paracasei</i>) had a higher survival rate (82.78%) in gastric juice and good tolerance to bile salt, and can be colonized in HT-29 cells. <i>L. paracasei</i> had a remarkable inhibitive activity of α-amylase (82.21%), α-glucosidase (84.29%) and dipeptidyl peptidase 4 (42.51%). <i>L. paracasei</i> had better scavenging activity of free radicals, total antioxidant activity (FRAP) and superoxide dismutase activity. According to the scores of the PCA, <i>L. paracasei</i> had the best hypoglycemic ability, and <i>L</i><i>actococcus lactis</i> (<i>L. lactis</i>) had the highest probiotic property. According to AHP, <i>L. paracasei</i> was the best potential hypoglycemic probiotic; furthermore, <i>L. lactis</i> showed the highest comprehensive performance except <i>Lactobacillus</i>. All lactic acid bacteria in this test had good safety. <i>L. paracasei</i> is expected to become a new potential hypoglycemic strain.https://www.mdpi.com/2304-8158/11/9/1363probiotic propertyhypoglycemic abilityantioxidant activitylactic acid bacteriacomprehensive evaluation |
| spellingShingle | Hongyu Wang Liang Li Comprehensive Evaluation of Probiotic Property, Hypoglycemic Ability and Antioxidant Activity of Lactic Acid Bacteria Foods probiotic property hypoglycemic ability antioxidant activity lactic acid bacteria comprehensive evaluation |
| title | Comprehensive Evaluation of Probiotic Property, Hypoglycemic Ability and Antioxidant Activity of Lactic Acid Bacteria |
| title_full | Comprehensive Evaluation of Probiotic Property, Hypoglycemic Ability and Antioxidant Activity of Lactic Acid Bacteria |
| title_fullStr | Comprehensive Evaluation of Probiotic Property, Hypoglycemic Ability and Antioxidant Activity of Lactic Acid Bacteria |
| title_full_unstemmed | Comprehensive Evaluation of Probiotic Property, Hypoglycemic Ability and Antioxidant Activity of Lactic Acid Bacteria |
| title_short | Comprehensive Evaluation of Probiotic Property, Hypoglycemic Ability and Antioxidant Activity of Lactic Acid Bacteria |
| title_sort | comprehensive evaluation of probiotic property hypoglycemic ability and antioxidant activity of lactic acid bacteria |
| topic | probiotic property hypoglycemic ability antioxidant activity lactic acid bacteria comprehensive evaluation |
| url | https://www.mdpi.com/2304-8158/11/9/1363 |
| work_keys_str_mv | AT hongyuwang comprehensiveevaluationofprobioticpropertyhypoglycemicabilityandantioxidantactivityoflacticacidbacteria AT liangli comprehensiveevaluationofprobioticpropertyhypoglycemicabilityandantioxidantactivityoflacticacidbacteria |