The Emergence of Carbapenem- and Colistin-Resistant Enterobacteria in Senegal
Antibiotic resistance is a public health problem. The emergence of carbapenemase-producing Enterobacterales (CPE) infections is a concern, particularly in Senegal. (1) Methods: Between January 2019 and July 2022, 240 isolates of enterobacteria resistant to third-generation cephalosporins and imipene...
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MDPI AG
2023-07-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/8/974 |
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| author | Habibou Sarr Aissatou Ahmet Niang Amadou Diop Oleg Mediannikov Hanane Zerrouki Seydina M. Diene Seynabou Lo Mouhamadou Lamine Dia Ahmad Iyane Sow Florence Fenollar Jean-Marc Rolain Linda Hadjadj |
| author_facet | Habibou Sarr Aissatou Ahmet Niang Amadou Diop Oleg Mediannikov Hanane Zerrouki Seydina M. Diene Seynabou Lo Mouhamadou Lamine Dia Ahmad Iyane Sow Florence Fenollar Jean-Marc Rolain Linda Hadjadj |
| author_sort | Habibou Sarr |
| collection | DOAJ |
| description | Antibiotic resistance is a public health problem. The emergence of carbapenemase-producing Enterobacterales (CPE) infections is a concern, particularly in Senegal. (1) Methods: Between January 2019 and July 2022, 240 isolates of enterobacteria resistant to third-generation cephalosporins and imipenem from biological samples from Fann Hospital (Dakar) and Hôpital Paix (Ziguinchor) were selected. The isolates were identified by MALDI-TOF mass spectrometry, and susceptibility tests were performed by the disk diffusion method. Antibiotic-resistance genes for class A beta-lactamases, carbapenemases, and plasmid resistance to colistin resistance (<i>mcr-1–8</i>) were screened by RT-PCR. (2) Results: The 240 enterobacteria were composed of: <i>Escherichia coli</i> (60.83%), <i>Klebsiella pneumoniae</i> (21.67%), <i>Enterobacter cloacae</i> (13.75%), <i>Citrobacter freundii</i> (2.08%), <i>Serratia marcescens</i> (0.83%), <i>Klebsiella aerogenes</i> (0.42%), and <i>Proteus mirabilis</i> (0.42%). Class A beta-lactamase genes were found in 229 isolates (70.41% <i>bla</i><sub>TEM</sub>, 37.5% <i>bla</i><sub>SHV</sub>, 83.75% <i>bla</i><sub>CTX-A</sub>, and 0.42% <i>bla</i><sub>CTX-B</sub>). The carbapenemase genes <i>bla</i><sub>OXA-48</sub> and <i>bla</i><sub>NDM</sub> were found in 25 isolates, including 14 isolates with <i>bla</i><sub>OXA-48</sub>, 13 isolates with <i>bla</i><sub>NDM</sub>, and 2 isolates with both genes simultaneously. The <i>mcr-8</i> gene was found in one isolate of <i>E. cloacae</i>. (3) Conclusions: The epidemiology of antibiotic-resistance genes in enterobacteria in Senegal shows the emergence of CPEs. This phenomenon is worrying, and rigorous surveillance is necessary to avoid further spread. |
| first_indexed | 2024-03-10T23:40:45Z |
| format | Article |
| id | doaj.art-a725a3906629461e9c9e4d1a00615288 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T23:40:45Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-a725a3906629461e9c9e4d1a006152882023-11-19T02:31:35ZengMDPI AGPathogens2076-08172023-07-0112897410.3390/pathogens12080974The Emergence of Carbapenem- and Colistin-Resistant Enterobacteria in SenegalHabibou Sarr0Aissatou Ahmet Niang1Amadou Diop2Oleg Mediannikov3Hanane Zerrouki4Seydina M. Diene5Seynabou Lo6Mouhamadou Lamine Dia7Ahmad Iyane Sow8Florence Fenollar9Jean-Marc Rolain10Linda Hadjadj11UFR des Sciences de la Santé, Université Assane Seck de Ziguinchor, Ziguinchor BP 523, SenegalFaculté de Médecine, Pharmacie et Odonto-Stomatologie, Université Cheikh-Anta-Diop, Dakar BP 5005, SenegalFaculté de Médecine, Pharmacie et Odonto-Stomatologie, Université Cheikh-Anta-Diop, Dakar BP 5005, SenegalFaculté de Médecine et de Pharmacie, MEPHI IRD, APHM, Aix Marseille Université, 13005 Marseille, FranceFaculté de Médecine et de Pharmacie, MEPHI IRD, APHM, Aix Marseille Université, 13005 Marseille, FranceFaculté de Médecine et de Pharmacie, MEPHI IRD, APHM, Aix Marseille Université, 13005 Marseille, FranceUFR des Sciences de la Santé, Université Gaston Berger, Saint Louis BP 234, SenegalFaculté de Médecine, Pharmacie et Odonto-Stomatologie, Université Cheikh-Anta-Diop, Dakar BP 5005, SenegalFaculté de Médecine, Pharmacie et Odonto-Stomatologie, Université Cheikh-Anta-Diop, Dakar BP 5005, SenegalIHU Méditerranée Infection, 13005 Marseille, FranceFaculté de Médecine et de Pharmacie, MEPHI IRD, APHM, Aix Marseille Université, 13005 Marseille, FranceFaculté de Médecine et de Pharmacie, MEPHI IRD, APHM, Aix Marseille Université, 13005 Marseille, FranceAntibiotic resistance is a public health problem. The emergence of carbapenemase-producing Enterobacterales (CPE) infections is a concern, particularly in Senegal. (1) Methods: Between January 2019 and July 2022, 240 isolates of enterobacteria resistant to third-generation cephalosporins and imipenem from biological samples from Fann Hospital (Dakar) and Hôpital Paix (Ziguinchor) were selected. The isolates were identified by MALDI-TOF mass spectrometry, and susceptibility tests were performed by the disk diffusion method. Antibiotic-resistance genes for class A beta-lactamases, carbapenemases, and plasmid resistance to colistin resistance (<i>mcr-1–8</i>) were screened by RT-PCR. (2) Results: The 240 enterobacteria were composed of: <i>Escherichia coli</i> (60.83%), <i>Klebsiella pneumoniae</i> (21.67%), <i>Enterobacter cloacae</i> (13.75%), <i>Citrobacter freundii</i> (2.08%), <i>Serratia marcescens</i> (0.83%), <i>Klebsiella aerogenes</i> (0.42%), and <i>Proteus mirabilis</i> (0.42%). Class A beta-lactamase genes were found in 229 isolates (70.41% <i>bla</i><sub>TEM</sub>, 37.5% <i>bla</i><sub>SHV</sub>, 83.75% <i>bla</i><sub>CTX-A</sub>, and 0.42% <i>bla</i><sub>CTX-B</sub>). The carbapenemase genes <i>bla</i><sub>OXA-48</sub> and <i>bla</i><sub>NDM</sub> were found in 25 isolates, including 14 isolates with <i>bla</i><sub>OXA-48</sub>, 13 isolates with <i>bla</i><sub>NDM</sub>, and 2 isolates with both genes simultaneously. The <i>mcr-8</i> gene was found in one isolate of <i>E. cloacae</i>. (3) Conclusions: The epidemiology of antibiotic-resistance genes in enterobacteria in Senegal shows the emergence of CPEs. This phenomenon is worrying, and rigorous surveillance is necessary to avoid further spread.https://www.mdpi.com/2076-0817/12/8/974enterobacteriacarbapenemasecolistin |
| spellingShingle | Habibou Sarr Aissatou Ahmet Niang Amadou Diop Oleg Mediannikov Hanane Zerrouki Seydina M. Diene Seynabou Lo Mouhamadou Lamine Dia Ahmad Iyane Sow Florence Fenollar Jean-Marc Rolain Linda Hadjadj The Emergence of Carbapenem- and Colistin-Resistant Enterobacteria in Senegal Pathogens enterobacteria carbapenemase colistin |
| title | The Emergence of Carbapenem- and Colistin-Resistant Enterobacteria in Senegal |
| title_full | The Emergence of Carbapenem- and Colistin-Resistant Enterobacteria in Senegal |
| title_fullStr | The Emergence of Carbapenem- and Colistin-Resistant Enterobacteria in Senegal |
| title_full_unstemmed | The Emergence of Carbapenem- and Colistin-Resistant Enterobacteria in Senegal |
| title_short | The Emergence of Carbapenem- and Colistin-Resistant Enterobacteria in Senegal |
| title_sort | emergence of carbapenem and colistin resistant enterobacteria in senegal |
| topic | enterobacteria carbapenemase colistin |
| url | https://www.mdpi.com/2076-0817/12/8/974 |
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