Wide-Field Fluorescence Microscopy of Real-Time Bioconjugation Sensing
We apply wide-field fluorescence microscopy to measure real-time attachment of photosynthetic proteins to plasmonically active silver nanowires. The observation of this effect is enabled, on the one hand, by sensitive detection of fluorescence and, on the other hand, by plasmonic enhancement of prot...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-01-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/18/1/290 |
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| author | Marcin Szalkowski Karolina Sulowska Justyna Grzelak Joanna Niedziółka-Jönsson Ewa Roźniecka Dorota Kowalska Sebastian Maćkowski |
| author_facet | Marcin Szalkowski Karolina Sulowska Justyna Grzelak Joanna Niedziółka-Jönsson Ewa Roźniecka Dorota Kowalska Sebastian Maćkowski |
| author_sort | Marcin Szalkowski |
| collection | DOAJ |
| description | We apply wide-field fluorescence microscopy to measure real-time attachment of photosynthetic proteins to plasmonically active silver nanowires. The observation of this effect is enabled, on the one hand, by sensitive detection of fluorescence and, on the other hand, by plasmonic enhancement of protein fluorescence. We examined two sample configurations with substrates being a bare glass coverslip and a coverslip functionalized with a monolayer of streptavidin. The different preparation of the substrate changes the observed behavior as far as attachment of the protein is concerned as well as its subsequent photobleaching. For the latter substrate the conjugation process is measurably slower. The described method can be universally applied in studying protein-nanostructure interactions for real-time fluorescence-based sensing. |
| first_indexed | 2024-04-11T18:05:32Z |
| format | Article |
| id | doaj.art-a72885d571d941ceb7db47cd742ffbaf |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T18:05:32Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-a72885d571d941ceb7db47cd742ffbaf2022-12-22T04:10:21ZengMDPI AGSensors1424-82202018-01-0118129010.3390/s18010290s18010290Wide-Field Fluorescence Microscopy of Real-Time Bioconjugation SensingMarcin Szalkowski0Karolina Sulowska1Justyna Grzelak2Joanna Niedziółka-Jönsson3Ewa Roźniecka4Dorota Kowalska5Sebastian Maćkowski6Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziadzka 5, 87-100 Torun, PolandFaculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziadzka 5, 87-100 Torun, PolandFaculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziadzka 5, 87-100 Torun, PolandInstitute of Physical Chemistry Polish Academy of Sciences, ul. Kasprzaka 44/52, 01-224 Warsaw, PolandInstitute of Physical Chemistry Polish Academy of Sciences, ul. Kasprzaka 44/52, 01-224 Warsaw, PolandFaculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziadzka 5, 87-100 Torun, PolandFaculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University, Grudziadzka 5, 87-100 Torun, PolandWe apply wide-field fluorescence microscopy to measure real-time attachment of photosynthetic proteins to plasmonically active silver nanowires. The observation of this effect is enabled, on the one hand, by sensitive detection of fluorescence and, on the other hand, by plasmonic enhancement of protein fluorescence. We examined two sample configurations with substrates being a bare glass coverslip and a coverslip functionalized with a monolayer of streptavidin. The different preparation of the substrate changes the observed behavior as far as attachment of the protein is concerned as well as its subsequent photobleaching. For the latter substrate the conjugation process is measurably slower. The described method can be universally applied in studying protein-nanostructure interactions for real-time fluorescence-based sensing.http://www.mdpi.com/1424-8220/18/1/290fluorescence imagingbioconjugationplasmon enhancementsilver nanowire |
| spellingShingle | Marcin Szalkowski Karolina Sulowska Justyna Grzelak Joanna Niedziółka-Jönsson Ewa Roźniecka Dorota Kowalska Sebastian Maćkowski Wide-Field Fluorescence Microscopy of Real-Time Bioconjugation Sensing Sensors fluorescence imaging bioconjugation plasmon enhancement silver nanowire |
| title | Wide-Field Fluorescence Microscopy of Real-Time Bioconjugation Sensing |
| title_full | Wide-Field Fluorescence Microscopy of Real-Time Bioconjugation Sensing |
| title_fullStr | Wide-Field Fluorescence Microscopy of Real-Time Bioconjugation Sensing |
| title_full_unstemmed | Wide-Field Fluorescence Microscopy of Real-Time Bioconjugation Sensing |
| title_short | Wide-Field Fluorescence Microscopy of Real-Time Bioconjugation Sensing |
| title_sort | wide field fluorescence microscopy of real time bioconjugation sensing |
| topic | fluorescence imaging bioconjugation plasmon enhancement silver nanowire |
| url | http://www.mdpi.com/1424-8220/18/1/290 |
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