Effects of S-Allyl-Cysteine on Survival, Apoptosis, and Proliferation of Leishmania Major in Vitro
Background: Many studies have suggested the benefits of garlic oil extracts against Leishmania parasites. Recent research has proved that these derivatives (such as allicin) are cytotoxic and therapeutic effects of garlic are in fact due to an odorless compound (S-allyl-cysteine). Considering the sm...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2013-04-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/2542 |
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| author | Gilda Eslami Ali Shams Mehran Fasahat Hassan Ashoori Zahra Hatefi Yasamin Nabipour Farzaneh Mirzaei Seyed Hossein Hejazi |
| author_facet | Gilda Eslami Ali Shams Mehran Fasahat Hassan Ashoori Zahra Hatefi Yasamin Nabipour Farzaneh Mirzaei Seyed Hossein Hejazi |
| author_sort | Gilda Eslami |
| collection | DOAJ |
| description | Background: Many studies have suggested the benefits of garlic oil extracts against Leishmania parasites. Recent research has proved that these derivatives (such as allicin) are cytotoxic and therapeutic effects of garlic are in fact due to an odorless compound (S-allyl-cysteine). Considering the small number of studies in this field, we evaluated the effectiveness of S-allyl-cysteine on survival, apoptosis, and proliferation of Leishmania major promastigotes in vitro.
Methods: In this study Leishmania major promastigotes (MRHO/IR/75/ER) were used. The parasites were first cultured and then treated with S-allyl-cysteine at end concentrations of 2.5, 5.0, 10.0, 20.0, 40.0, and 50.0 mM. Their survival, apoptosis, and proliferation was assessed after 72 hours.
Findings: None of the concentrations of S-allyl-cysteine could induce apoptosis in parasite promastigotes. Lower concentrations (5.0 and 10.0 mM) of S-allyl-cysteine had greatest effect on proliferation. On the other hand, direct observation showed that cultures comprising S-allyl-cysteine at end concentration of 20.0, 40.0, and 50.0 mM formed more rosettes.
Conclusion: The antioxidant effects of S-allyl-cysteine have been previously proved. Similarly, this study showed that some concentrations of S-allyl-cysteine could affect the survival and growth of promastigotes in culture medium. Therefore, this substance seems to be a good stimulating factor for growth of parasite promastigotes in culture media. |
| first_indexed | 2024-03-12T18:51:49Z |
| format | Article |
| id | doaj.art-a729760839dd41db90b306f1772c55c2 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T18:51:49Z |
| publishDate | 2013-04-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-a729760839dd41db90b306f1772c55c22023-08-02T07:11:54ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2013-04-01312251211301282Effects of S-Allyl-Cysteine on Survival, Apoptosis, and Proliferation of Leishmania Major in VitroGilda Eslami0Ali Shams1Mehran Fasahat2Hassan Ashoori3Zahra Hatefi4Yasamin Nabipour5Farzaneh Mirzaei6Seyed Hossein Hejazi7Assistant Professor, Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranAssistant Professor, Department of Immunology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranStudent, Department of Laboratory Sciences, Student Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, IranStudent, Department of Laboratory Sciences, Student Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, IranStudent, Department of Laboratory Sciences, Student Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, IranMSc Student, Department of Parasitology, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranAssociate Professor, Skin Diseases and Leishmaniasis Research Center AND Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: Many studies have suggested the benefits of garlic oil extracts against Leishmania parasites. Recent research has proved that these derivatives (such as allicin) are cytotoxic and therapeutic effects of garlic are in fact due to an odorless compound (S-allyl-cysteine). Considering the small number of studies in this field, we evaluated the effectiveness of S-allyl-cysteine on survival, apoptosis, and proliferation of Leishmania major promastigotes in vitro. Methods: In this study Leishmania major promastigotes (MRHO/IR/75/ER) were used. The parasites were first cultured and then treated with S-allyl-cysteine at end concentrations of 2.5, 5.0, 10.0, 20.0, 40.0, and 50.0 mM. Their survival, apoptosis, and proliferation was assessed after 72 hours. Findings: None of the concentrations of S-allyl-cysteine could induce apoptosis in parasite promastigotes. Lower concentrations (5.0 and 10.0 mM) of S-allyl-cysteine had greatest effect on proliferation. On the other hand, direct observation showed that cultures comprising S-allyl-cysteine at end concentration of 20.0, 40.0, and 50.0 mM formed more rosettes. Conclusion: The antioxidant effects of S-allyl-cysteine have been previously proved. Similarly, this study showed that some concentrations of S-allyl-cysteine could affect the survival and growth of promastigotes in culture medium. Therefore, this substance seems to be a good stimulating factor for growth of parasite promastigotes in culture media.http://jims.mui.ac.ir/index.php/jims/article/view/2542Leishmania majorS-allyl-cysteineApoptosisProliferation |
| spellingShingle | Gilda Eslami Ali Shams Mehran Fasahat Hassan Ashoori Zahra Hatefi Yasamin Nabipour Farzaneh Mirzaei Seyed Hossein Hejazi Effects of S-Allyl-Cysteine on Survival, Apoptosis, and Proliferation of Leishmania Major in Vitro مجله دانشکده پزشکی اصفهان Leishmania major S-allyl-cysteine Apoptosis Proliferation |
| title | Effects of S-Allyl-Cysteine on Survival, Apoptosis, and Proliferation of Leishmania Major in Vitro |
| title_full | Effects of S-Allyl-Cysteine on Survival, Apoptosis, and Proliferation of Leishmania Major in Vitro |
| title_fullStr | Effects of S-Allyl-Cysteine on Survival, Apoptosis, and Proliferation of Leishmania Major in Vitro |
| title_full_unstemmed | Effects of S-Allyl-Cysteine on Survival, Apoptosis, and Proliferation of Leishmania Major in Vitro |
| title_short | Effects of S-Allyl-Cysteine on Survival, Apoptosis, and Proliferation of Leishmania Major in Vitro |
| title_sort | effects of s allyl cysteine on survival apoptosis and proliferation of leishmania major in vitro |
| topic | Leishmania major S-allyl-cysteine Apoptosis Proliferation |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/2542 |
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