Deciphering Plant-Induced Responses toward <i>Botrytis cinerea</i> and <i>Plasmopara viticola</i> Attacks in Two Grapevine Cultivars Colonized by the Root Biocontrol Oomycete, <i>Pythium oligandrum</i>
Two major diseases that affect grapevine leaves and berries are controlled by the oomycete <i>Pythium oligandrum</i>. As the efficacy of biocontrol agents strongly depends on factors such as the trophic behaviors of pathogens and cultivar susceptibility, a two-disease approach was implem...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/5/511 |
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| author | Amira Yacoub Rana Haidar Ouiza Mesguida Jonathan Gerbore Maya Hachicha Eléonore Attard Rémy Guyoneaud Patrice Rey |
| author_facet | Amira Yacoub Rana Haidar Ouiza Mesguida Jonathan Gerbore Maya Hachicha Eléonore Attard Rémy Guyoneaud Patrice Rey |
| author_sort | Amira Yacoub |
| collection | DOAJ |
| description | Two major diseases that affect grapevine leaves and berries are controlled by the oomycete <i>Pythium oligandrum</i>. As the efficacy of biocontrol agents strongly depends on factors such as the trophic behaviors of pathogens and cultivar susceptibility, a two-disease approach was implemented to evaluate the activity of <i>P. oligandrum</i> against <i>Botrytis cinerea</i> (the necrotrophic fungus of gray mold) and <i>Plasmopara viticola</i> (the biotrophic oomycete of downy mildew) on two grapevine cultivars with different susceptibilities to these two pathogens. The results show that grapevine root inoculation with <i>P. oligandrum</i> significantly reduced <i>P. viticola</i> and <i>B. cinerea</i> infection on the leaves of the two cultivars, but with differences. This was observed when the relative expression of 10 genes was measured in response to each pathogen, and could be attributed to their lifestyles, i.e., biotrophic or necrotrophic, which are related to the activation of specific metabolic pathways of the plant. In response to <i>P. viticola</i> infection, genes from the jasmonate and ethylene pathways were mainly induced, whereas for <i>B. cinerea</i>, the genes induced were those of the ethylene–jasmonate pathway. The different levels of defense against <i>B. cinerea</i> and <i>P. viticola</i> could also explain the difference in cultivar susceptibility to these pathogens. |
| first_indexed | 2024-03-11T03:35:44Z |
| format | Article |
| id | doaj.art-a72a9da9e14f4feca977954701d22cdb |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-11T03:35:44Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-a72a9da9e14f4feca977954701d22cdb2023-11-18T02:01:31ZengMDPI AGJournal of Fungi2309-608X2023-04-019551110.3390/jof9050511Deciphering Plant-Induced Responses toward <i>Botrytis cinerea</i> and <i>Plasmopara viticola</i> Attacks in Two Grapevine Cultivars Colonized by the Root Biocontrol Oomycete, <i>Pythium oligandrum</i>Amira Yacoub0Rana Haidar1Ouiza Mesguida2Jonathan Gerbore3Maya Hachicha4Eléonore Attard5Rémy Guyoneaud6Patrice Rey7E2S UPPA, CNRS, IPREM, Université de Pau et des Pays de l’Adour, 64000 Pau, FranceE2S UPPA, CNRS, IPREM, Université de Pau et des Pays de l’Adour, 64000 Pau, FranceE2S UPPA, CNRS, IPREM, Université de Pau et des Pays de l’Adour, 64000 Pau, FranceGreenCell, Biopôle Clermont-Limagne, 63360 Saint Beauzire, FranceINRAE, UMR1065, Santé et Agroécologie du Vignoble (SAVE), ISVV, 33883 Villenave d’Ornon, FranceE2S UPPA, CNRS, IPREM, Université de Pau et des Pays de l’Adour, 64000 Pau, FranceE2S UPPA, CNRS, IPREM, Université de Pau et des Pays de l’Adour, 64000 Pau, FranceE2S UPPA, CNRS, IPREM, Université de Pau et des Pays de l’Adour, 64000 Pau, FranceTwo major diseases that affect grapevine leaves and berries are controlled by the oomycete <i>Pythium oligandrum</i>. As the efficacy of biocontrol agents strongly depends on factors such as the trophic behaviors of pathogens and cultivar susceptibility, a two-disease approach was implemented to evaluate the activity of <i>P. oligandrum</i> against <i>Botrytis cinerea</i> (the necrotrophic fungus of gray mold) and <i>Plasmopara viticola</i> (the biotrophic oomycete of downy mildew) on two grapevine cultivars with different susceptibilities to these two pathogens. The results show that grapevine root inoculation with <i>P. oligandrum</i> significantly reduced <i>P. viticola</i> and <i>B. cinerea</i> infection on the leaves of the two cultivars, but with differences. This was observed when the relative expression of 10 genes was measured in response to each pathogen, and could be attributed to their lifestyles, i.e., biotrophic or necrotrophic, which are related to the activation of specific metabolic pathways of the plant. In response to <i>P. viticola</i> infection, genes from the jasmonate and ethylene pathways were mainly induced, whereas for <i>B. cinerea</i>, the genes induced were those of the ethylene–jasmonate pathway. The different levels of defense against <i>B. cinerea</i> and <i>P. viticola</i> could also explain the difference in cultivar susceptibility to these pathogens.https://www.mdpi.com/2309-608X/9/5/511grapevinebiocontroloomycetecultivarsinduction of resistance |
| spellingShingle | Amira Yacoub Rana Haidar Ouiza Mesguida Jonathan Gerbore Maya Hachicha Eléonore Attard Rémy Guyoneaud Patrice Rey Deciphering Plant-Induced Responses toward <i>Botrytis cinerea</i> and <i>Plasmopara viticola</i> Attacks in Two Grapevine Cultivars Colonized by the Root Biocontrol Oomycete, <i>Pythium oligandrum</i> Journal of Fungi grapevine biocontrol oomycete cultivars induction of resistance |
| title | Deciphering Plant-Induced Responses toward <i>Botrytis cinerea</i> and <i>Plasmopara viticola</i> Attacks in Two Grapevine Cultivars Colonized by the Root Biocontrol Oomycete, <i>Pythium oligandrum</i> |
| title_full | Deciphering Plant-Induced Responses toward <i>Botrytis cinerea</i> and <i>Plasmopara viticola</i> Attacks in Two Grapevine Cultivars Colonized by the Root Biocontrol Oomycete, <i>Pythium oligandrum</i> |
| title_fullStr | Deciphering Plant-Induced Responses toward <i>Botrytis cinerea</i> and <i>Plasmopara viticola</i> Attacks in Two Grapevine Cultivars Colonized by the Root Biocontrol Oomycete, <i>Pythium oligandrum</i> |
| title_full_unstemmed | Deciphering Plant-Induced Responses toward <i>Botrytis cinerea</i> and <i>Plasmopara viticola</i> Attacks in Two Grapevine Cultivars Colonized by the Root Biocontrol Oomycete, <i>Pythium oligandrum</i> |
| title_short | Deciphering Plant-Induced Responses toward <i>Botrytis cinerea</i> and <i>Plasmopara viticola</i> Attacks in Two Grapevine Cultivars Colonized by the Root Biocontrol Oomycete, <i>Pythium oligandrum</i> |
| title_sort | deciphering plant induced responses toward i botrytis cinerea i and i plasmopara viticola i attacks in two grapevine cultivars colonized by the root biocontrol oomycete i pythium oligandrum i |
| topic | grapevine biocontrol oomycete cultivars induction of resistance |
| url | https://www.mdpi.com/2309-608X/9/5/511 |
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