SSR marker based DNA fingerprinting and diversity studies in mustard (Brassica juncea)
Seventeen mustard cultivars were subjected to SSR assay to identify the efficient primers for developing DNA fingerprinting. Two SSR primers of mustard Ra1H11 and Ra1F03 revealed a considerable DNA polymorphism of 41.17% and 35.29% respectively. The SSR primer Ra1H11 (0.772) of mustard had showed hi...
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| Format: | Article |
| Language: | English |
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Indian Society of Plant Breeders
2018-03-01
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| Series: | Electronic Journal of Plant Breeding |
| Subjects: | |
| Online Access: | http://ejplantbreeding.org/index.php/EJPB/article/view/2008 |
| _version_ | 1818511409833574400 |
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| author | Puranjaya Panigrahi Kaushik Kumar Panigrahi and Somnath Bhattacharya |
| author_facet | Puranjaya Panigrahi Kaushik Kumar Panigrahi and Somnath Bhattacharya |
| author_sort | Puranjaya Panigrahi |
| collection | DOAJ |
| description | Seventeen mustard cultivars were subjected to SSR assay to identify the efficient primers for developing DNA fingerprinting. Two SSR primers of mustard Ra1H11 and Ra1F03 revealed a considerable DNA polymorphism of 41.17% and 35.29% respectively. The SSR primer Ra1H11 (0.772) of mustard had showed highest discriminating power followed by Ra1F09 (0.617) then Ra1F03 (0.558), Ra2B02 (0.382) and Ra1G07 (0.228) respectively. The highest PIC value had showed by Ra1F03 (0.525), then followed by), Ra1H11 (0.363), Ra2B02 (0.359), Ra1F09 (0.290) respectively. The primer Ra1H11 had showed highest MI value (0.423) then followed by Ra1F03 (0.262), Ra1F09 (0.193), Ra2B02 (0.119) and Ra1G07 (0.071) respectively. Among all the mustard SSR primers Ra1H11, Ra1F09, Ra1F03 followed a descending order of their efficiency to discriminate the mustard cultivars so that they can be used for developing DNA finger printing patterns for these cultivars. A combination of SSR primer Ra1F03 with Ra1F09 had distinguished the cultivar Bhagirathi from Panchali and Sanjukta, Ra1F09 with Ra1H11 had distinguished the cultivar kranti from Agrani and PT-303, Ra1F03 with Ra1F09 had distinguished the cultivar JD-6 from Pusa Bold, and Ra1F03 with Ra1H11 had distinguished the cultivar YST-151 from Jhumuka by producing a unique banding pattern. |
| first_indexed | 2024-12-10T23:32:59Z |
| format | Article |
| id | doaj.art-a72b17fffb994ff59be157aed6540247 |
| institution | Directory Open Access Journal |
| issn | 0975-928X |
| language | English |
| last_indexed | 2024-12-10T23:32:59Z |
| publishDate | 2018-03-01 |
| publisher | Indian Society of Plant Breeders |
| record_format | Article |
| series | Electronic Journal of Plant Breeding |
| spelling | doaj.art-a72b17fffb994ff59be157aed65402472022-12-22T01:29:18ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2018-03-0191253710.5958/0975-928X.2018.00004.2SSR marker based DNA fingerprinting and diversity studies in mustard (Brassica juncea)Puranjaya PanigrahiKaushik Kumar Panigrahi andSomnath BhattacharyaSeventeen mustard cultivars were subjected to SSR assay to identify the efficient primers for developing DNA fingerprinting. Two SSR primers of mustard Ra1H11 and Ra1F03 revealed a considerable DNA polymorphism of 41.17% and 35.29% respectively. The SSR primer Ra1H11 (0.772) of mustard had showed highest discriminating power followed by Ra1F09 (0.617) then Ra1F03 (0.558), Ra2B02 (0.382) and Ra1G07 (0.228) respectively. The highest PIC value had showed by Ra1F03 (0.525), then followed by), Ra1H11 (0.363), Ra2B02 (0.359), Ra1F09 (0.290) respectively. The primer Ra1H11 had showed highest MI value (0.423) then followed by Ra1F03 (0.262), Ra1F09 (0.193), Ra2B02 (0.119) and Ra1G07 (0.071) respectively. Among all the mustard SSR primers Ra1H11, Ra1F09, Ra1F03 followed a descending order of their efficiency to discriminate the mustard cultivars so that they can be used for developing DNA finger printing patterns for these cultivars. A combination of SSR primer Ra1F03 with Ra1F09 had distinguished the cultivar Bhagirathi from Panchali and Sanjukta, Ra1F09 with Ra1H11 had distinguished the cultivar kranti from Agrani and PT-303, Ra1F03 with Ra1F09 had distinguished the cultivar JD-6 from Pusa Bold, and Ra1F03 with Ra1H11 had distinguished the cultivar YST-151 from Jhumuka by producing a unique banding pattern.http://ejplantbreeding.org/index.php/EJPB/article/view/2008mustarddna fingerprintinggenetic diversityssr markers |
| spellingShingle | Puranjaya Panigrahi Kaushik Kumar Panigrahi and Somnath Bhattacharya SSR marker based DNA fingerprinting and diversity studies in mustard (Brassica juncea) Electronic Journal of Plant Breeding mustard dna fingerprinting genetic diversity ssr markers |
| title | SSR marker based DNA fingerprinting and diversity studies in mustard (Brassica juncea) |
| title_full | SSR marker based DNA fingerprinting and diversity studies in mustard (Brassica juncea) |
| title_fullStr | SSR marker based DNA fingerprinting and diversity studies in mustard (Brassica juncea) |
| title_full_unstemmed | SSR marker based DNA fingerprinting and diversity studies in mustard (Brassica juncea) |
| title_short | SSR marker based DNA fingerprinting and diversity studies in mustard (Brassica juncea) |
| title_sort | ssr marker based dna fingerprinting and diversity studies in mustard brassica juncea |
| topic | mustard dna fingerprinting genetic diversity ssr markers |
| url | http://ejplantbreeding.org/index.php/EJPB/article/view/2008 |
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