Design and Fabrication of a Fully-Integrated, Miniaturised Fluidic System for the Analysis of Enzyme Kinetics
The lab-on-a-chip concept, enabled by microfluidic technology, promises the integration of multiple discrete laboratory techniques into a miniaturised system. Research into microfluidics has generally focused on the development of individual elements of the total system (often with relatively limite...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Micromachines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-666X/14/3/537 |
| _version_ | 1827748579384492032 |
|---|---|
| author | Andreas Tsiamis Anthony Buchoux Stephen T. Mahon Anthony J. Walton Stewart Smith David J. Clarke Adam A. Stokes |
| author_facet | Andreas Tsiamis Anthony Buchoux Stephen T. Mahon Anthony J. Walton Stewart Smith David J. Clarke Adam A. Stokes |
| author_sort | Andreas Tsiamis |
| collection | DOAJ |
| description | The lab-on-a-chip concept, enabled by microfluidic technology, promises the integration of multiple discrete laboratory techniques into a miniaturised system. Research into microfluidics has generally focused on the development of individual elements of the total system (often with relatively limited functionality), without full consideration for integration into a complete fully optimised and miniaturised system. Typically, the operation of many of the reported lab-on-a-chip devices is dependent on the support of a laboratory framework. In this paper, a demonstrator platform for routine laboratory analysis is designed and built, which fully integrates a number of technologies into a single device with multiple domains such as fluidics, electronics, pneumatics, hydraulics, and photonics. This facilitates the delivery of breakthroughs in research, by incorporating all physical requirements into a single device. To highlight this proposed approach, this demonstrator microsystem acts as a fully integrated biochemical assay reaction system. The resulting design determines enzyme kinetics in an automated process and combines reservoirs, three-dimensional fluidic channels, optical sensing, and electronics in a low-cost, low-power and portable package. |
| first_indexed | 2024-03-11T06:10:35Z |
| format | Article |
| id | doaj.art-a72c7c4011044c4d8596c3971b18e8e1 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-11T06:10:35Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-a72c7c4011044c4d8596c3971b18e8e12023-11-17T12:42:12ZengMDPI AGMicromachines2072-666X2023-02-0114353710.3390/mi14030537Design and Fabrication of a Fully-Integrated, Miniaturised Fluidic System for the Analysis of Enzyme KineticsAndreas Tsiamis0Anthony Buchoux1Stephen T. Mahon2Anthony J. Walton3Stewart Smith4David J. Clarke5Adam A. Stokes6School of Engineering, Institute for Integrated Micro and Nano Systems, The University of Edinburgh, The King’s Buildings, Edinburgh EH9 3FF, UKSchool of Engineering, Institute for Multiscale Thermofluids, The University of Edinburgh, The King’s Buildings, Edinburgh EH9 3LJ, UKSchool of Engineering, Institute for Integrated Micro and Nano Systems, The University of Edinburgh, The King’s Buildings, Edinburgh EH9 3FF, UKSchool of Engineering, Institute for Integrated Micro and Nano Systems, The University of Edinburgh, The King’s Buildings, Edinburgh EH9 3FF, UKSchool of Engineering, Institute for Bio-Engineering, The University of Edinburgh, The King’s Buildings, Edinburgh EH9 3FF, UKEaStCHEM School of Chemistry, The University of Edinburgh, Edinburgh EH9 3FJ, UKSchool of Engineering, Institute for Integrated Micro and Nano Systems, The University of Edinburgh, The King’s Buildings, Edinburgh EH9 3FF, UKThe lab-on-a-chip concept, enabled by microfluidic technology, promises the integration of multiple discrete laboratory techniques into a miniaturised system. Research into microfluidics has generally focused on the development of individual elements of the total system (often with relatively limited functionality), without full consideration for integration into a complete fully optimised and miniaturised system. Typically, the operation of many of the reported lab-on-a-chip devices is dependent on the support of a laboratory framework. In this paper, a demonstrator platform for routine laboratory analysis is designed and built, which fully integrates a number of technologies into a single device with multiple domains such as fluidics, electronics, pneumatics, hydraulics, and photonics. This facilitates the delivery of breakthroughs in research, by incorporating all physical requirements into a single device. To highlight this proposed approach, this demonstrator microsystem acts as a fully integrated biochemical assay reaction system. The resulting design determines enzyme kinetics in an automated process and combines reservoirs, three-dimensional fluidic channels, optical sensing, and electronics in a low-cost, low-power and portable package.https://www.mdpi.com/2072-666X/14/3/537sensorsfluidicsintegrationlab-on-a-chipintegrated devicesminiaturised total analysis system |
| spellingShingle | Andreas Tsiamis Anthony Buchoux Stephen T. Mahon Anthony J. Walton Stewart Smith David J. Clarke Adam A. Stokes Design and Fabrication of a Fully-Integrated, Miniaturised Fluidic System for the Analysis of Enzyme Kinetics Micromachines sensors fluidics integration lab-on-a-chip integrated devices miniaturised total analysis system |
| title | Design and Fabrication of a Fully-Integrated, Miniaturised Fluidic System for the Analysis of Enzyme Kinetics |
| title_full | Design and Fabrication of a Fully-Integrated, Miniaturised Fluidic System for the Analysis of Enzyme Kinetics |
| title_fullStr | Design and Fabrication of a Fully-Integrated, Miniaturised Fluidic System for the Analysis of Enzyme Kinetics |
| title_full_unstemmed | Design and Fabrication of a Fully-Integrated, Miniaturised Fluidic System for the Analysis of Enzyme Kinetics |
| title_short | Design and Fabrication of a Fully-Integrated, Miniaturised Fluidic System for the Analysis of Enzyme Kinetics |
| title_sort | design and fabrication of a fully integrated miniaturised fluidic system for the analysis of enzyme kinetics |
| topic | sensors fluidics integration lab-on-a-chip integrated devices miniaturised total analysis system |
| url | https://www.mdpi.com/2072-666X/14/3/537 |
| work_keys_str_mv | AT andreastsiamis designandfabricationofafullyintegratedminiaturisedfluidicsystemfortheanalysisofenzymekinetics AT anthonybuchoux designandfabricationofafullyintegratedminiaturisedfluidicsystemfortheanalysisofenzymekinetics AT stephentmahon designandfabricationofafullyintegratedminiaturisedfluidicsystemfortheanalysisofenzymekinetics AT anthonyjwalton designandfabricationofafullyintegratedminiaturisedfluidicsystemfortheanalysisofenzymekinetics AT stewartsmith designandfabricationofafullyintegratedminiaturisedfluidicsystemfortheanalysisofenzymekinetics AT davidjclarke designandfabricationofafullyintegratedminiaturisedfluidicsystemfortheanalysisofenzymekinetics AT adamastokes designandfabricationofafullyintegratedminiaturisedfluidicsystemfortheanalysisofenzymekinetics |