Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i>
Endophytic microorganisms are found within the tissues of many plants species, with some conferring several benefits to the host plant including resistance to plant diseases. In this study, two putative endophytic fungi that were previously isolated from wild seeds of <i>Brassica</i>, id...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2387 |
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| author | Davood Roodi James P. Millner Craig R. McGill Richard D. Johnson Shen-Yan Hea Jenny J. Brookes Travis R. Glare Stuart D. Card |
| author_facet | Davood Roodi James P. Millner Craig R. McGill Richard D. Johnson Shen-Yan Hea Jenny J. Brookes Travis R. Glare Stuart D. Card |
| author_sort | Davood Roodi |
| collection | DOAJ |
| description | Endophytic microorganisms are found within the tissues of many plants species, with some conferring several benefits to the host plant including resistance to plant diseases. In this study, two putative endophytic fungi that were previously isolated from wild seeds of <i>Brassica</i>, identified as <i>Beauveria bassiana</i> and <i>Pseudogymnoascus pannorum</i>, were inoculated into cultivars of three <i>Brassica</i> species—<i>Brassica napus</i>, <i>Br. rapa</i> and <i>Br. oleracea</i>. Both fungal endophytes were reisolated from above- and below-ground tissues of inoculated plants at four different plant-growth stages, including cotyledon, one-leaf, two-leaf, and four-leaf stages. None of the plants colonised by these fungi exhibited any obvious disease symptoms, indicating the formation of novel mutualistic associations. These novel plant–endophyte associations formed between <i>Brassica</i> plants and <i>Be. bassiana</i> significantly inhibited phoma stem canker, a devastating disease of <i>Brassica</i> crops worldwide, caused by the fungal pathogen <i>Leptosphaeria maculans</i>. The novel association formed with <i>P. pannorum</i> significantly suppressed the amount of disease caused by <i>L. maculans</i> in one out of two experiments. Although biological control is not a new strategy, endophytic fungi with both antiinsect and antifungal activity are a highly conceivable, sustainable option to manage pests and diseases of economically important crops. |
| first_indexed | 2024-03-10T05:14:59Z |
| format | Article |
| id | doaj.art-a72dd5d17f684de691ece20b4cf70461 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:14:59Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a72dd5d17f684de691ece20b4cf704612023-11-23T00:30:42ZengMDPI AGMicroorganisms2076-26072021-11-01911238710.3390/microorganisms9112387Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i>Davood Roodi0James P. Millner1Craig R. McGill2Richard D. Johnson3Shen-Yan Hea4Jenny J. Brookes5Travis R. Glare6Stuart D. Card7Resilient Agriculture, AgResearch Limited, Grasslands Research Centre, Private Bag 11008, Palmerston North 4410, New ZealandSchool of Agriculture & Environment, Massey University, Private Bag 11222, Palmerston North 4442, New ZealandSchool of Agriculture & Environment, Massey University, Private Bag 11222, Palmerston North 4442, New ZealandResilient Agriculture, AgResearch Limited, Grasslands Research Centre, Private Bag 11008, Palmerston North 4410, New ZealandDigital Agriculture, AgResearch Limited, Invermay Agricultural Centre, Private Bag 50034, Mosgiel 9053, New ZealandBio-Protection Research Centre, P.O. Box 85084, Lincoln University, Lincoln 7647, New ZealandBio-Protection Research Centre, P.O. Box 85084, Lincoln University, Lincoln 7647, New ZealandResilient Agriculture, AgResearch Limited, Grasslands Research Centre, Private Bag 11008, Palmerston North 4410, New ZealandEndophytic microorganisms are found within the tissues of many plants species, with some conferring several benefits to the host plant including resistance to plant diseases. In this study, two putative endophytic fungi that were previously isolated from wild seeds of <i>Brassica</i>, identified as <i>Beauveria bassiana</i> and <i>Pseudogymnoascus pannorum</i>, were inoculated into cultivars of three <i>Brassica</i> species—<i>Brassica napus</i>, <i>Br. rapa</i> and <i>Br. oleracea</i>. Both fungal endophytes were reisolated from above- and below-ground tissues of inoculated plants at four different plant-growth stages, including cotyledon, one-leaf, two-leaf, and four-leaf stages. None of the plants colonised by these fungi exhibited any obvious disease symptoms, indicating the formation of novel mutualistic associations. These novel plant–endophyte associations formed between <i>Brassica</i> plants and <i>Be. bassiana</i> significantly inhibited phoma stem canker, a devastating disease of <i>Brassica</i> crops worldwide, caused by the fungal pathogen <i>Leptosphaeria maculans</i>. The novel association formed with <i>P. pannorum</i> significantly suppressed the amount of disease caused by <i>L. maculans</i> in one out of two experiments. Although biological control is not a new strategy, endophytic fungi with both antiinsect and antifungal activity are a highly conceivable, sustainable option to manage pests and diseases of economically important crops.https://www.mdpi.com/2076-2607/9/11/2387<i>Beauveria bassiana</i>biological controlbrassicaceaecordycipitaceaeleptosphaeriaceae<i>Leptosphaeria maculans</i> |
| spellingShingle | Davood Roodi James P. Millner Craig R. McGill Richard D. Johnson Shen-Yan Hea Jenny J. Brookes Travis R. Glare Stuart D. Card Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i> Microorganisms <i>Beauveria bassiana</i> biological control brassicaceae cordycipitaceae leptosphaeriaceae <i>Leptosphaeria maculans</i> |
| title | Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i> |
| title_full | Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i> |
| title_fullStr | Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i> |
| title_full_unstemmed | Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i> |
| title_short | Development of Plant–Fungal Endophyte Associations to Suppress Phoma Stem Canker in <i>Brassica</i> |
| title_sort | development of plant fungal endophyte associations to suppress phoma stem canker in i brassica i |
| topic | <i>Beauveria bassiana</i> biological control brassicaceae cordycipitaceae leptosphaeriaceae <i>Leptosphaeria maculans</i> |
| url | https://www.mdpi.com/2076-2607/9/11/2387 |
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