Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies
In this study, we present a review on a useful approach, namely, immunoaffinity column coupled with monoclonal antibodies (MAbs), to separate natural compounds and its application for cell-based studies. The immunoaffinity column aids in separating the specific target compound from the crude extract...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Antibodies |
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| Online Access: | https://www.mdpi.com/2073-4468/10/4/48 |
| _version_ | 1797506885987860480 |
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| author | Takuhiro Uto Tomoe Ohta Shunsuke Fujii Yukihiro Shoyama |
| author_facet | Takuhiro Uto Tomoe Ohta Shunsuke Fujii Yukihiro Shoyama |
| author_sort | Takuhiro Uto |
| collection | DOAJ |
| description | In this study, we present a review on a useful approach, namely, immunoaffinity column coupled with monoclonal antibodies (MAbs), to separate natural compounds and its application for cell-based studies. The immunoaffinity column aids in separating the specific target compound from the crude extract. The column capacity was stable even after more than 10 purification cycles of use under the same conditions. After applying the crude extract to the column, the column was washed with washing buffer and eluted with elution buffer. The elution fraction contained the target compound bound to MAb, whereas the washing fraction was the crude extract, which contained all compounds except a group of target compounds; therefore, the washing fraction was referred to as a knockout (KO) crude extract. Cell-based studies using the KO extract revealed the actual effects of the natural compounds in the crude extract. One-step separation of natural compounds using the immunoaffinity column coupled with MAbs may help in determining the potential functions of natural compounds in crude extracts. |
| first_indexed | 2024-03-10T04:38:50Z |
| format | Article |
| id | doaj.art-a73087f66904468a8c8aaf43befb4bf1 |
| institution | Directory Open Access Journal |
| issn | 2073-4468 |
| language | English |
| last_indexed | 2024-03-10T04:38:50Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibodies |
| spelling | doaj.art-a73087f66904468a8c8aaf43befb4bf12023-11-23T03:31:57ZengMDPI AGAntibodies2073-44682021-12-011044810.3390/antib10040048Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based StudiesTakuhiro Uto0Tomoe Ohta1Shunsuke Fujii2Yukihiro Shoyama3Graduate School of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch-Cho, Sasebo 859-3298, JapanGraduate School of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch-Cho, Sasebo 859-3298, JapanFaculty of Health Management, Nagasaki International University, 2825-7 Huis Ten Bosch-Cho, Sasebo 859-3298, JapanGraduate School of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch-Cho, Sasebo 859-3298, JapanIn this study, we present a review on a useful approach, namely, immunoaffinity column coupled with monoclonal antibodies (MAbs), to separate natural compounds and its application for cell-based studies. The immunoaffinity column aids in separating the specific target compound from the crude extract. The column capacity was stable even after more than 10 purification cycles of use under the same conditions. After applying the crude extract to the column, the column was washed with washing buffer and eluted with elution buffer. The elution fraction contained the target compound bound to MAb, whereas the washing fraction was the crude extract, which contained all compounds except a group of target compounds; therefore, the washing fraction was referred to as a knockout (KO) crude extract. Cell-based studies using the KO extract revealed the actual effects of the natural compounds in the crude extract. One-step separation of natural compounds using the immunoaffinity column coupled with MAbs may help in determining the potential functions of natural compounds in crude extracts.https://www.mdpi.com/2073-4468/10/4/48monoclonal antibodyimmunoaffinity columnnatural compoundone-step separation methodcrude extractknockout extract |
| spellingShingle | Takuhiro Uto Tomoe Ohta Shunsuke Fujii Yukihiro Shoyama Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies Antibodies monoclonal antibody immunoaffinity column natural compound one-step separation method crude extract knockout extract |
| title | Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies |
| title_full | Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies |
| title_fullStr | Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies |
| title_full_unstemmed | Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies |
| title_short | Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies |
| title_sort | immunological separation of bioactive natural compounds from crude drug extract and its application for cell based studies |
| topic | monoclonal antibody immunoaffinity column natural compound one-step separation method crude extract knockout extract |
| url | https://www.mdpi.com/2073-4468/10/4/48 |
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