Characterization of the Second Generation Cryopreserved Dendrobium Bobby Messina Using Histological and RAPD Analyses
This study was conducted to detect the morphological, histological and molecular diff erences in the second generation of the PVS2 cryopreserved Dendrobium Bobby Messina [DBM] (18 months old culture) plantlets. Morphological analyses indicated that similarit...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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National University of Mongolia
2015-12-01
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| Series: | Mongolian Journal of Biological Sciences |
| Subjects: | |
| Online Access: | http://mjbs.num.edu.mn/uploads/files/MJBS%20Volume%2013%20Number%201-2%20December%202015/PDF/mjbs-13,13-18-antony_2015.pdf |
| _version_ | 1818577810583715840 |
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| author | Jessica Jeyanthi James Antony Leong Kah Wai Yungeree Oyunbileg Sreeramanan Subramaniam |
| author_facet | Jessica Jeyanthi James Antony Leong Kah Wai Yungeree Oyunbileg Sreeramanan Subramaniam |
| author_sort | Jessica Jeyanthi James Antony |
| collection | DOAJ |
| description | This study was conducted to detect the morphological, histological and molecular
diff erences in the second generation of the PVS2 cryopreserved
Dendrobium
Bobby Messina [DBM] (18 months old culture) plantlets. Morphological analyses
indicated that similarities and diff erences in cryopreserved DBM plantlets comparing
to control stock culture based on selected morphological criteria. Morphological
criteria, such as root length, number of shoot per explant and shoot length displayed
diff erences, while the other three criteria, leaf diameter, leaf length and PLBs size
were similar in cryopreserved compared to the control stock culture plant. Higher
amount of homogenous cell population and denser cytoplasm were observed in
cryopreserved PLBs compared to control stock culture PLBs based on histological
analysis. This suggests the existance of somatic embryogenesis development
mechanism taking place during the recovery and regeneration of the cryopreserved
PLBs. However, RAPD analyses based on 10 primers indicated that cryopreserved
DBM regenerated from vitrifi cation method generated a total of 20 to 39.9%
polymorphic bands as compared to stock culture indicating potential somaclonal
variation. Hence, an increase percentage of polymorphics bands in cryopreserved
plantlets 18 months post cryopreservation as compared to previous report of 10%
polymorphic bands in cryopreserved DBM 3 months post cryopreservation. |
| first_indexed | 2024-12-16T06:35:50Z |
| format | Article |
| id | doaj.art-a73116b2171e4f5db73df3aa6ef1f025 |
| institution | Directory Open Access Journal |
| issn | 1684-3908 2225-4994 |
| language | English |
| last_indexed | 2024-12-16T06:35:50Z |
| publishDate | 2015-12-01 |
| publisher | National University of Mongolia |
| record_format | Article |
| series | Mongolian Journal of Biological Sciences |
| spelling | doaj.art-a73116b2171e4f5db73df3aa6ef1f0252022-12-21T22:40:47ZengNational University of MongoliaMongolian Journal of Biological Sciences1684-39082225-49942015-12-01131-2131810.22353/mjbs.2015.13.02Characterization of the Second Generation Cryopreserved Dendrobium Bobby Messina Using Histological and RAPD AnalysesJessica Jeyanthi James Antony0Leong Kah Wai1Yungeree Oyunbileg2Sreeramanan Subramaniam3School of Biological Sciences, Universiti Sains Malaysia, Georgetown, 11800, Penang, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, Georgetown, 11800, Penang, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, Georgetown, 11800, Penang, Malaysia2 Institute of General and Experimental Biology, Mongolian Academy of Sciences, Peace avenue-54B, Ulaanbaatar 13330, MongoliaThis study was conducted to detect the morphological, histological and molecular diff erences in the second generation of the PVS2 cryopreserved Dendrobium Bobby Messina [DBM] (18 months old culture) plantlets. Morphological analyses indicated that similarities and diff erences in cryopreserved DBM plantlets comparing to control stock culture based on selected morphological criteria. Morphological criteria, such as root length, number of shoot per explant and shoot length displayed diff erences, while the other three criteria, leaf diameter, leaf length and PLBs size were similar in cryopreserved compared to the control stock culture plant. Higher amount of homogenous cell population and denser cytoplasm were observed in cryopreserved PLBs compared to control stock culture PLBs based on histological analysis. This suggests the existance of somatic embryogenesis development mechanism taking place during the recovery and regeneration of the cryopreserved PLBs. However, RAPD analyses based on 10 primers indicated that cryopreserved DBM regenerated from vitrifi cation method generated a total of 20 to 39.9% polymorphic bands as compared to stock culture indicating potential somaclonal variation. Hence, an increase percentage of polymorphics bands in cryopreserved plantlets 18 months post cryopreservation as compared to previous report of 10% polymorphic bands in cryopreserved DBM 3 months post cryopreservation.http://mjbs.num.edu.mn/uploads/files/MJBS%20Volume%2013%20Number%201-2%20December%202015/PDF/mjbs-13,13-18-antony_2015.pdfmorphologyhistologymolecular analysisDendrobium |
| spellingShingle | Jessica Jeyanthi James Antony Leong Kah Wai Yungeree Oyunbileg Sreeramanan Subramaniam Characterization of the Second Generation Cryopreserved Dendrobium Bobby Messina Using Histological and RAPD Analyses Mongolian Journal of Biological Sciences morphology histology molecular analysis Dendrobium |
| title | Characterization of the Second Generation Cryopreserved Dendrobium Bobby Messina Using Histological and RAPD Analyses |
| title_full | Characterization of the Second Generation Cryopreserved Dendrobium Bobby Messina Using Histological and RAPD Analyses |
| title_fullStr | Characterization of the Second Generation Cryopreserved Dendrobium Bobby Messina Using Histological and RAPD Analyses |
| title_full_unstemmed | Characterization of the Second Generation Cryopreserved Dendrobium Bobby Messina Using Histological and RAPD Analyses |
| title_short | Characterization of the Second Generation Cryopreserved Dendrobium Bobby Messina Using Histological and RAPD Analyses |
| title_sort | characterization of the second generation cryopreserved dendrobium bobby messina using histological and rapd analyses |
| topic | morphology histology molecular analysis Dendrobium |
| url | http://mjbs.num.edu.mn/uploads/files/MJBS%20Volume%2013%20Number%201-2%20December%202015/PDF/mjbs-13,13-18-antony_2015.pdf |
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