Aerobic Exercise Training Improves Calcium Handling and Cardiac Function in Rats with Heart Failure Resulting from Aortic Stenosis
Aerobic exercise training (AET) has been used to manage heart disease. AET may totally or partially restore the activity and/or expression of proteins that regulate calcium (Ca<sup>2+</sup>) handling, optimize intracellular Ca<sup>2+</sup> flow, and attenuate cardiac function...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/15/12306 |
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| author | Vítor Loureiro da Silva Gustavo Augusto Ferreira Mota Sérgio Luiz Borges de Souza Dijon Henrique Salomé de Campos Alexandre Barroso Melo Danielle Fernandes Vileigas Priscila Murucci Coelho Paula Grippa Sant’Ana Carlos Padovani Ana Paula Lima-Leopoldo Silméia Garcia Zanati Bazan André Soares Leopoldo Antonio Carlos Cicogna |
| author_facet | Vítor Loureiro da Silva Gustavo Augusto Ferreira Mota Sérgio Luiz Borges de Souza Dijon Henrique Salomé de Campos Alexandre Barroso Melo Danielle Fernandes Vileigas Priscila Murucci Coelho Paula Grippa Sant’Ana Carlos Padovani Ana Paula Lima-Leopoldo Silméia Garcia Zanati Bazan André Soares Leopoldo Antonio Carlos Cicogna |
| author_sort | Vítor Loureiro da Silva |
| collection | DOAJ |
| description | Aerobic exercise training (AET) has been used to manage heart disease. AET may totally or partially restore the activity and/or expression of proteins that regulate calcium (Ca<sup>2+</sup>) handling, optimize intracellular Ca<sup>2+</sup> flow, and attenuate cardiac functional impairment in failing hearts. However, the literature presents conflicting data regarding the effects of AET on Ca<sup>2+</sup> transit and cardiac function in rats with heart failure resulting from aortic stenosis (AoS). This study aimed to evaluate the impact of AET on Ca<sup>2+</sup> handling and cardiac function in rats with heart failure due to AoS. Wistar rats were distributed into two groups: control (Sham; <i>n</i> = 61) and aortic stenosis (AoS; <i>n</i> = 44). After 18 weeks, the groups were redistributed into: non-exposed to exercise training (Sham, <i>n</i> = 28 and AoS, <i>n</i> = 22) and trained (Sham-ET, <i>n</i> = 33 and AoS-ET, <i>n</i> = 22) for 10 weeks. Treadmill exercise training was performed with a velocity equivalent to the lactate threshold. The cardiac function was analyzed by echocardiogram, isolated papillary muscles, and isolated cardiomyocytes. During assays of isolated papillary muscles and isolated cardiomyocytes, the Ca<sup>2+</sup> concentrations were evaluated. The expression of regulatory proteins for diastolic Ca<sup>2+</sup> was assessed via Western Blot. AET attenuated the diastolic dysfunction and improved the systolic function. AoS-ET animals presented an enhanced response to post-rest contraction and SERCA2a and L-type Ca<sup>2+</sup> channel blockage compared to the AoS. Furthermore, AET was able to improve aspects of the mechanical function and the responsiveness of the myofilaments to the Ca<sup>2+</sup> of the AoS-ET animals. AoS animals presented an alteration in the protein expression of SERCA2a and NCX, and AET restored SERCA2a and NCX levels near normal values. Therefore, AET increased SERCA2a activity and myofilament responsiveness to Ca<sup>2+</sup> and improved the cellular Ca<sup>2+</sup> influx mechanism, attenuating cardiac dysfunction at cellular, tissue, and chamber levels in animals with AoS and heart failure. |
| first_indexed | 2024-03-11T00:25:41Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T00:25:41Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a732c212945347c482d1325e62c461922023-11-18T23:03:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124151230610.3390/ijms241512306Aerobic Exercise Training Improves Calcium Handling and Cardiac Function in Rats with Heart Failure Resulting from Aortic StenosisVítor Loureiro da Silva0Gustavo Augusto Ferreira Mota1Sérgio Luiz Borges de Souza2Dijon Henrique Salomé de Campos3Alexandre Barroso Melo4Danielle Fernandes Vileigas5Priscila Murucci Coelho6Paula Grippa Sant’Ana7Carlos Padovani8Ana Paula Lima-Leopoldo9Silméia Garcia Zanati Bazan10André Soares Leopoldo11Antonio Carlos Cicogna12Department of Internal Medicine, Botucatu Medical School, São Paulo State University (UNESP), Botucatu 18618-687, BrazilDepartment of Internal Medicine, Botucatu Medical School, São Paulo State University (UNESP), Botucatu 18618-687, BrazilDepartment of Internal Medicine, Botucatu Medical School, São Paulo State University (UNESP), Botucatu 18618-687, BrazilDepartment of Internal Medicine, Botucatu Medical School, São Paulo State University (UNESP), Botucatu 18618-687, BrazilDepartment of Sports, Federal University of Espirito Santo, Vitória 29075-910, BrazilDepartment of Internal Medicine, Botucatu Medical School, São Paulo State University (UNESP), Botucatu 18618-687, BrazilDepartment of Sports, Federal University of Espirito Santo, Vitória 29075-910, BrazilDepartment of Internal Medicine, Botucatu Medical School, São Paulo State University (UNESP), Botucatu 18618-687, BrazilDepartment of Biostatistics, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-689, BrazilDepartment of Sports, Federal University of Espirito Santo, Vitória 29075-910, BrazilDepartment of Internal Medicine, Botucatu Medical School, São Paulo State University (UNESP), Botucatu 18618-687, BrazilDepartment of Sports, Federal University of Espirito Santo, Vitória 29075-910, BrazilDepartment of Internal Medicine, Botucatu Medical School, São Paulo State University (UNESP), Botucatu 18618-687, BrazilAerobic exercise training (AET) has been used to manage heart disease. AET may totally or partially restore the activity and/or expression of proteins that regulate calcium (Ca<sup>2+</sup>) handling, optimize intracellular Ca<sup>2+</sup> flow, and attenuate cardiac functional impairment in failing hearts. However, the literature presents conflicting data regarding the effects of AET on Ca<sup>2+</sup> transit and cardiac function in rats with heart failure resulting from aortic stenosis (AoS). This study aimed to evaluate the impact of AET on Ca<sup>2+</sup> handling and cardiac function in rats with heart failure due to AoS. Wistar rats were distributed into two groups: control (Sham; <i>n</i> = 61) and aortic stenosis (AoS; <i>n</i> = 44). After 18 weeks, the groups were redistributed into: non-exposed to exercise training (Sham, <i>n</i> = 28 and AoS, <i>n</i> = 22) and trained (Sham-ET, <i>n</i> = 33 and AoS-ET, <i>n</i> = 22) for 10 weeks. Treadmill exercise training was performed with a velocity equivalent to the lactate threshold. The cardiac function was analyzed by echocardiogram, isolated papillary muscles, and isolated cardiomyocytes. During assays of isolated papillary muscles and isolated cardiomyocytes, the Ca<sup>2+</sup> concentrations were evaluated. The expression of regulatory proteins for diastolic Ca<sup>2+</sup> was assessed via Western Blot. AET attenuated the diastolic dysfunction and improved the systolic function. AoS-ET animals presented an enhanced response to post-rest contraction and SERCA2a and L-type Ca<sup>2+</sup> channel blockage compared to the AoS. Furthermore, AET was able to improve aspects of the mechanical function and the responsiveness of the myofilaments to the Ca<sup>2+</sup> of the AoS-ET animals. AoS animals presented an alteration in the protein expression of SERCA2a and NCX, and AET restored SERCA2a and NCX levels near normal values. Therefore, AET increased SERCA2a activity and myofilament responsiveness to Ca<sup>2+</sup> and improved the cellular Ca<sup>2+</sup> influx mechanism, attenuating cardiac dysfunction at cellular, tissue, and chamber levels in animals with AoS and heart failure.https://www.mdpi.com/1422-0067/24/15/12306exercise trainingaortic stenosisheart failureisolated papillary muscleisolated cardiomyocytecalcium handling |
| spellingShingle | Vítor Loureiro da Silva Gustavo Augusto Ferreira Mota Sérgio Luiz Borges de Souza Dijon Henrique Salomé de Campos Alexandre Barroso Melo Danielle Fernandes Vileigas Priscila Murucci Coelho Paula Grippa Sant’Ana Carlos Padovani Ana Paula Lima-Leopoldo Silméia Garcia Zanati Bazan André Soares Leopoldo Antonio Carlos Cicogna Aerobic Exercise Training Improves Calcium Handling and Cardiac Function in Rats with Heart Failure Resulting from Aortic Stenosis International Journal of Molecular Sciences exercise training aortic stenosis heart failure isolated papillary muscle isolated cardiomyocyte calcium handling |
| title | Aerobic Exercise Training Improves Calcium Handling and Cardiac Function in Rats with Heart Failure Resulting from Aortic Stenosis |
| title_full | Aerobic Exercise Training Improves Calcium Handling and Cardiac Function in Rats with Heart Failure Resulting from Aortic Stenosis |
| title_fullStr | Aerobic Exercise Training Improves Calcium Handling and Cardiac Function in Rats with Heart Failure Resulting from Aortic Stenosis |
| title_full_unstemmed | Aerobic Exercise Training Improves Calcium Handling and Cardiac Function in Rats with Heart Failure Resulting from Aortic Stenosis |
| title_short | Aerobic Exercise Training Improves Calcium Handling and Cardiac Function in Rats with Heart Failure Resulting from Aortic Stenosis |
| title_sort | aerobic exercise training improves calcium handling and cardiac function in rats with heart failure resulting from aortic stenosis |
| topic | exercise training aortic stenosis heart failure isolated papillary muscle isolated cardiomyocyte calcium handling |
| url | https://www.mdpi.com/1422-0067/24/15/12306 |
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