Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goat
Objectives: This study aimed to examine the impacts of the wide range of concentrations of glu¬cose and trehalose on the tris-citric acid-egg yolk-fructose (TCEF) extenders for cryopreservation of goat semen. Materials and Methods: The sperm sample was pooled, washed, and diluted in control (TCEF w...
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| Format: | Article |
| Language: | English |
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Network for the Veterinarians of Bangladesh
2023-06-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
| Subjects: | |
| Online Access: | https://bdvets.org/JAVAR/?mno=148349 |
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| author | Md. Mostofa Kamal Md. Emtiaj Alam Sunny Kumar Das Most. Shorifa Yeasmin Soshe Ahmed Mst. Afroza Rahman Dipak Kumar Das Md. Royhan Gofur Md. Abdul Masum |
| author_facet | Md. Mostofa Kamal Md. Emtiaj Alam Sunny Kumar Das Most. Shorifa Yeasmin Soshe Ahmed Mst. Afroza Rahman Dipak Kumar Das Md. Royhan Gofur Md. Abdul Masum |
| author_sort | Md. Mostofa Kamal |
| collection | DOAJ |
| description | Objectives: This study aimed to examine the impacts of the wide range of concentrations of glu¬cose and trehalose on the tris-citric acid-egg yolk-fructose (TCEF) extenders for cryopreservation of goat semen.
Materials and Methods: The sperm sample was pooled, washed, and diluted in control (TCEF without glucose and trehalose), TCEF + glucose (75, 150, 450, and 900 mm), and TCEF + trehalose (75, 150, 450, and 900 mm). After equilibrations, the semen straws were frozen under LN2 in the LN2 tank. After LN2 storage, the straws were thawed at 37°C for 30 seconds. The sperm parame¬ters of all study groups were checked after equilibration and freezing.
Results: After equilibration, the progressive motility (PM), total motility (TM), and viability of sperm in G-75, G-150, G-450, T-75, T-150, and T-450 were not significantly different (p < 0.05) from those in control. After cryopreservation and thawing, the PM, TM, and plasma membrane integrity (PMI) of T-150 were significantly higher (p < 0.05) than in control, G-75, G-900, T-75, and T-900. The viability of sperm in T-150 was substantially higher (p < 0.05) than in the control, whereas there was no significant difference among the control, G-75, G-900, T-75, and T-900. However, the acrosome integrity (AI) of sperm in G-900 was significantly decreased (p < 0.05) compared to the control, G-75, G-150, G-450, T-75, T-150, and T-450.
Conclusion: According to the findings, the supplementation of 150 mm trehalose in the TCEF dilu¬ent was more efficient for sperm cryopreservation in the buck as reflected by PM, TM, viability, PMI, and AI. [J Adv Vet Anim Res 2023; 10(2.000): 169-177] |
| first_indexed | 2024-03-12T22:25:55Z |
| format | Article |
| id | doaj.art-a733a2f259994c2294f2c9ef75139ed2 |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-03-12T22:25:55Z |
| publishDate | 2023-06-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-a733a2f259994c2294f2c9ef75139ed22023-07-22T06:03:10ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102023-06-0110216917710.5455/javar.2023.j666148349Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goatMd. Mostofa Kamal0Md. Emtiaj Alam1Sunny Kumar Das2Most. Shorifa Yeasmin3Soshe Ahmed4Mst. Afroza Rahman5Dipak Kumar Das6Md. Royhan Gofur7Md. Abdul MasumDepartment of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi, Bangladesh Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi, Bangladesh Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi, Bangladesh Department of Pharmacy, Faculty of Sciences and Engineering, East West University, Dhaka, Bangladesh Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi, Bangladesh Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi, Bangladesh Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi, Bangladesh Department of Anatomy, Histology and Physiology, Faculty of Animal Science and Veterinary Medicine, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.Objectives: This study aimed to examine the impacts of the wide range of concentrations of glu¬cose and trehalose on the tris-citric acid-egg yolk-fructose (TCEF) extenders for cryopreservation of goat semen. Materials and Methods: The sperm sample was pooled, washed, and diluted in control (TCEF without glucose and trehalose), TCEF + glucose (75, 150, 450, and 900 mm), and TCEF + trehalose (75, 150, 450, and 900 mm). After equilibrations, the semen straws were frozen under LN2 in the LN2 tank. After LN2 storage, the straws were thawed at 37°C for 30 seconds. The sperm parame¬ters of all study groups were checked after equilibration and freezing. Results: After equilibration, the progressive motility (PM), total motility (TM), and viability of sperm in G-75, G-150, G-450, T-75, T-150, and T-450 were not significantly different (p < 0.05) from those in control. After cryopreservation and thawing, the PM, TM, and plasma membrane integrity (PMI) of T-150 were significantly higher (p < 0.05) than in control, G-75, G-900, T-75, and T-900. The viability of sperm in T-150 was substantially higher (p < 0.05) than in the control, whereas there was no significant difference among the control, G-75, G-900, T-75, and T-900. However, the acrosome integrity (AI) of sperm in G-900 was significantly decreased (p < 0.05) compared to the control, G-75, G-150, G-450, T-75, T-150, and T-450. Conclusion: According to the findings, the supplementation of 150 mm trehalose in the TCEF dilu¬ent was more efficient for sperm cryopreservation in the buck as reflected by PM, TM, viability, PMI, and AI. [J Adv Vet Anim Res 2023; 10(2.000): 169-177]https://bdvets.org/JAVAR/?mno=148349buck; cryopreservation; glucose; sperm; sperm parameter; trehalose |
| spellingShingle | Md. Mostofa Kamal Md. Emtiaj Alam Sunny Kumar Das Most. Shorifa Yeasmin Soshe Ahmed Mst. Afroza Rahman Dipak Kumar Das Md. Royhan Gofur Md. Abdul Masum Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goat Journal of Advanced Veterinary and Animal Research buck; cryopreservation; glucose; sperm; sperm parameter; trehalose |
| title | Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goat |
| title_full | Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goat |
| title_fullStr | Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goat |
| title_full_unstemmed | Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goat |
| title_short | Effects of glucose and trehalose on tris-citric acid-egg yolk-fructose diluents for semen cryopreservation in goat |
| title_sort | effects of glucose and trehalose on tris citric acid egg yolk fructose diluents for semen cryopreservation in goat |
| topic | buck; cryopreservation; glucose; sperm; sperm parameter; trehalose |
| url | https://bdvets.org/JAVAR/?mno=148349 |
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