Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro
<i>Background and objectives:</i> There are several studies that sevoflurane could enhance proliferation of cancer cells, while others suggest no effect on clinical outcome. We conducted in vivo and in vitro experiments to investigate the effects of sevoflurane, a volatile anesthetic, on...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-01-01
|
| Series: | Medicina |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1010-660X/57/1/45 |
| _version_ | 1797728636621553664 |
|---|---|
| author | Yeojung Kim Sangwon Yun Keun-A Shin Woosuk Chung Youngkwon Ko Yoon-Hee Kim Chaeseong Lim |
| author_facet | Yeojung Kim Sangwon Yun Keun-A Shin Woosuk Chung Youngkwon Ko Yoon-Hee Kim Chaeseong Lim |
| author_sort | Yeojung Kim |
| collection | DOAJ |
| description | <i>Background and objectives:</i> There are several studies that sevoflurane could enhance proliferation of cancer cells, while others suggest no effect on clinical outcome. We conducted in vivo and in vitro experiments to investigate the effects of sevoflurane, a volatile anesthetic, on proliferation and outcomes of Lewis lung carcinoma (LLC) cells. <i>Materials and Methods:</i> A total of 37 mice were injected with LLC cells to compare the tumor size and survival of the sevoflurane exposed group (sevo group) and control group. The sevo group was exposed to 2% sevoflurane and 4 L/min of oxygen for 1 h per day 3 times per week, and the control group was exposed only to 4 L/min of oxygen. In vitro study, 12 plates incubated with LCC cells. 6 plates were exposed to 2% sevoflurane for 1 hr/day for 3 days and 6 plates were not exposed, and cell proliferation was compared after 3 days. <i>Results:</i> There were no significant differences in survival or tumor size between mice exposed to sevoflurane and control mice (survival: 29.06 ± 4.45 vs. 28.76 ± 3.75, <i>p</i> = 0.836; tumor size: 0.75 (0.41–1.02) vs. 0.49 (0.11–0.79), <i>p</i> = 0.153). However, in vitro study, the proliferation of LLC cells exposed to sevoflurane increased by 9.2% compared to the control group (<i>p</i> = 0.018). <i>Conclusions:</i> Sevoflurane (2 vol%) exposure could promote proliferation of LLC cells in vitro environment, but may not affect proliferation of LLC cells in vivo environment. These results suggest that in vitro studies on the effects of anesthetics on cancer may differ from those of in vivo or clinical studies. |
| first_indexed | 2024-03-12T11:16:58Z |
| format | Article |
| id | doaj.art-a73792b04a6d47339c4e2725c6435a19 |
| institution | Directory Open Access Journal |
| issn | 1010-660X |
| language | English |
| last_indexed | 2024-03-12T11:16:58Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-a73792b04a6d47339c4e2725c6435a192023-09-02T01:57:21ZengMDPI AGMedicina1010-660X2021-01-0157454510.3390/medicina57010045Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In VitroYeojung Kim0Sangwon Yun1Keun-A Shin2Woosuk Chung3Youngkwon Ko4Yoon-Hee Kim5Chaeseong Lim6Department of Anesthesiology and Pain Medicine, Chungnam National University Sejong Hospital, Sejong 30099, KoreaDepartment of Anesthesiology and Pain Medicine, Chungnam National University Hospital, Daejeon 35015, KoreaResearch Institute for Medical Sciences, Chungnam National University College of Medicine, Daejeon 35015, KoreaDepartment of Anesthesiology and Pain Medicine, Chungnam National University College of Medicine, Daejeon 35015, KoreaDepartment of Anesthesiology and Pain Medicine, Chungnam National University College of Medicine, Daejeon 35015, KoreaDepartment of Anesthesiology and Pain Medicine, Chungnam National University College of Medicine, Daejeon 35015, KoreaDepartment of Anesthesiology and Pain Medicine, Chungnam National University College of Medicine, Daejeon 35015, Korea<i>Background and objectives:</i> There are several studies that sevoflurane could enhance proliferation of cancer cells, while others suggest no effect on clinical outcome. We conducted in vivo and in vitro experiments to investigate the effects of sevoflurane, a volatile anesthetic, on proliferation and outcomes of Lewis lung carcinoma (LLC) cells. <i>Materials and Methods:</i> A total of 37 mice were injected with LLC cells to compare the tumor size and survival of the sevoflurane exposed group (sevo group) and control group. The sevo group was exposed to 2% sevoflurane and 4 L/min of oxygen for 1 h per day 3 times per week, and the control group was exposed only to 4 L/min of oxygen. In vitro study, 12 plates incubated with LCC cells. 6 plates were exposed to 2% sevoflurane for 1 hr/day for 3 days and 6 plates were not exposed, and cell proliferation was compared after 3 days. <i>Results:</i> There were no significant differences in survival or tumor size between mice exposed to sevoflurane and control mice (survival: 29.06 ± 4.45 vs. 28.76 ± 3.75, <i>p</i> = 0.836; tumor size: 0.75 (0.41–1.02) vs. 0.49 (0.11–0.79), <i>p</i> = 0.153). However, in vitro study, the proliferation of LLC cells exposed to sevoflurane increased by 9.2% compared to the control group (<i>p</i> = 0.018). <i>Conclusions:</i> Sevoflurane (2 vol%) exposure could promote proliferation of LLC cells in vitro environment, but may not affect proliferation of LLC cells in vivo environment. These results suggest that in vitro studies on the effects of anesthetics on cancer may differ from those of in vivo or clinical studies.https://www.mdpi.com/1010-660X/57/1/45sevofluraneLewis lung carcinomacanceranestheticssurvival |
| spellingShingle | Yeojung Kim Sangwon Yun Keun-A Shin Woosuk Chung Youngkwon Ko Yoon-Hee Kim Chaeseong Lim Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro Medicina sevoflurane Lewis lung carcinoma cancer anesthetics survival |
| title | Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro |
| title_full | Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro |
| title_fullStr | Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro |
| title_full_unstemmed | Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro |
| title_short | Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro |
| title_sort | effects of sevoflurane on lewis lung carcinoma cell proliferation in vivo and in vitro |
| topic | sevoflurane Lewis lung carcinoma cancer anesthetics survival |
| url | https://www.mdpi.com/1010-660X/57/1/45 |
| work_keys_str_mv | AT yeojungkim effectsofsevofluraneonlewislungcarcinomacellproliferationinvivoandinvitro AT sangwonyun effectsofsevofluraneonlewislungcarcinomacellproliferationinvivoandinvitro AT keunashin effectsofsevofluraneonlewislungcarcinomacellproliferationinvivoandinvitro AT woosukchung effectsofsevofluraneonlewislungcarcinomacellproliferationinvivoandinvitro AT youngkwonko effectsofsevofluraneonlewislungcarcinomacellproliferationinvivoandinvitro AT yoonheekim effectsofsevofluraneonlewislungcarcinomacellproliferationinvivoandinvitro AT chaeseonglim effectsofsevofluraneonlewislungcarcinomacellproliferationinvivoandinvitro |