The diagnostic value of serological studies in pediatric patients with acute Mycoplasma pneumoniae infection
Background: Mycoplasma pneumoniae is a common pathogen of respiratory tract infections in pediatric patients. Serological studies are traditional methods for the diagnosis. However, early diagnosis of M. pneumoniae infections remains problematic. We investigate the value of early serum immunoglobuli...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-04-01
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| Series: | Journal of Microbiology, Immunology and Infection |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118218300756 |
| _version_ | 1828345689674874880 |
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| author | Lih-Ju Lin Fu-Chieh Chang Hsin Chi Wai-Tim Jim Daniel Tsung-Ning Huang Yen-Hsin Kung Ching-Ying Huang Nan-Chang Chiu Lung Chang |
| author_facet | Lih-Ju Lin Fu-Chieh Chang Hsin Chi Wai-Tim Jim Daniel Tsung-Ning Huang Yen-Hsin Kung Ching-Ying Huang Nan-Chang Chiu Lung Chang |
| author_sort | Lih-Ju Lin |
| collection | DOAJ |
| description | Background: Mycoplasma pneumoniae is a common pathogen of respiratory tract infections in pediatric patients. Serological studies are traditional methods for the diagnosis. However, early diagnosis of M. pneumoniae infections remains problematic. We investigate the value of early serum immunoglobulin A (IgA), in addition to immunoglobulin G (IgG), and immunoglobulin M (IgM) levels, in children infected with M. pneumoniae. Methods: From August 2016 to February 2017, we enrolled pediatric patients based on both clinical symptoms and chest x-ray, and confirmed by positive throat culture for M. pneumoniae. Serum titers of M. pneumoniae IgM, IgG, and IgA during the acute phase were checked. All respiratory samples were further analyzed by polymerase chain reaction (PCR). Diagnostic values of different tests were evaluated. Results: Fifty-six patients fulfilled the diagnostic criteria, with a median age of 4.84 years. Most of them (89.3%) were enrolled within 7 days of disease onset. PCR was positive in 71.4% of the study population. Early IgG samples were of limited value in diagnosing M. pneumoniae infection, of which 89.3% showed a negative result. Positive rates of early serum IgA and IgM were 48.2% and 46.4%, respectively. In combination with IgA and/or IgM, the sensitivity increased to 71.4% during their early clinical course. Conclusions: In the pediatric population, combined serological tests of M. pneumoniae IgA and IgM, offer an accurate method of early diagnosis comparable to that of PCR, and can be an alternative choice for prompt detection of mycoplasma infections when PCR and culture are not available. Keywords: Mycoplasma pneumoniae, Children, Diagnosis, Culture, Serology |
| first_indexed | 2024-04-14T00:15:21Z |
| format | Article |
| id | doaj.art-a737bc8a7c2949fbb4853f24c9cd04c4 |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-04-14T00:15:21Z |
| publishDate | 2020-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-a737bc8a7c2949fbb4853f24c9cd04c42022-12-22T02:23:09ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822020-04-01532351356The diagnostic value of serological studies in pediatric patients with acute Mycoplasma pneumoniae infectionLih-Ju Lin0Fu-Chieh Chang1Hsin Chi2Wai-Tim Jim3Daniel Tsung-Ning Huang4Yen-Hsin Kung5Ching-Ying Huang6Nan-Chang Chiu7Lung Chang8Department of Pediatrics, MacKay Children's Hospital and MacKay Memorial Hospital, Taipei, Taiwan; Department of Pediatrics, Taiwan Adventist Hospital, Taipei, TaiwanInfection Control Center, MacKay Memorial Hospital, College of Management, Yuan Ze University, TaiwanDepartment of Pediatrics, MacKay Children's Hospital and MacKay Memorial Hospital, Taipei, Taiwan; Department of Medicine, MacKay Medicine College, New Taipei, Taiwan; MacKay Junior College of Medicine, Nursing and Management, Taipei, TaiwanDepartment of Pediatrics, MacKay Children's Hospital and MacKay Memorial Hospital, Taipei, Taiwan; Department of Medicine, MacKay Medicine College, New Taipei, Taiwan; MacKay Junior College of Medicine, Nursing and Management, Taipei, TaiwanDepartment of Pediatrics, MacKay Children's Hospital and MacKay Memorial Hospital, Taipei, TaiwanDepartment of Pediatrics, MacKay Children's Hospital and MacKay Memorial Hospital, Taipei, TaiwanDepartment of Pediatrics, MacKay Children's Hospital and MacKay Memorial Hospital, Taipei, Taiwan; Department of Medicine, MacKay Medicine College, New Taipei, TaiwanDepartment of Pediatrics, MacKay Children's Hospital and MacKay Memorial Hospital, Taipei, Taiwan; Department of Medicine, MacKay Medicine College, New Taipei, Taiwan; MacKay Junior College of Medicine, Nursing and Management, Taipei, Taiwan; Corresponding author. Department of Pediatrics, Mackay Memorial Hospital, No. 92, Sec. 2, Zhongshan N. Rd., 10449, Taipei, Taiwan. Fax: +886 2 25433642.Department of Pediatrics, MacKay Children's Hospital and MacKay Memorial Hospital, Taipei, Taiwan; Department of Medicine, MacKay Medicine College, New Taipei, Taiwan; MacKay Junior College of Medicine, Nursing and Management, Taipei, Taiwan; Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan; Corresponding author. Department of Pediatrics, Mackay Memorial Hospital, No. 92, Sec. 2, Zhongshan N. Rd., 10449, Taipei, Taiwan. Fax: +886 2 25433642.Background: Mycoplasma pneumoniae is a common pathogen of respiratory tract infections in pediatric patients. Serological studies are traditional methods for the diagnosis. However, early diagnosis of M. pneumoniae infections remains problematic. We investigate the value of early serum immunoglobulin A (IgA), in addition to immunoglobulin G (IgG), and immunoglobulin M (IgM) levels, in children infected with M. pneumoniae. Methods: From August 2016 to February 2017, we enrolled pediatric patients based on both clinical symptoms and chest x-ray, and confirmed by positive throat culture for M. pneumoniae. Serum titers of M. pneumoniae IgM, IgG, and IgA during the acute phase were checked. All respiratory samples were further analyzed by polymerase chain reaction (PCR). Diagnostic values of different tests were evaluated. Results: Fifty-six patients fulfilled the diagnostic criteria, with a median age of 4.84 years. Most of them (89.3%) were enrolled within 7 days of disease onset. PCR was positive in 71.4% of the study population. Early IgG samples were of limited value in diagnosing M. pneumoniae infection, of which 89.3% showed a negative result. Positive rates of early serum IgA and IgM were 48.2% and 46.4%, respectively. In combination with IgA and/or IgM, the sensitivity increased to 71.4% during their early clinical course. Conclusions: In the pediatric population, combined serological tests of M. pneumoniae IgA and IgM, offer an accurate method of early diagnosis comparable to that of PCR, and can be an alternative choice for prompt detection of mycoplasma infections when PCR and culture are not available. Keywords: Mycoplasma pneumoniae, Children, Diagnosis, Culture, Serologyhttp://www.sciencedirect.com/science/article/pii/S1684118218300756 |
| spellingShingle | Lih-Ju Lin Fu-Chieh Chang Hsin Chi Wai-Tim Jim Daniel Tsung-Ning Huang Yen-Hsin Kung Ching-Ying Huang Nan-Chang Chiu Lung Chang The diagnostic value of serological studies in pediatric patients with acute Mycoplasma pneumoniae infection Journal of Microbiology, Immunology and Infection |
| title | The diagnostic value of serological studies in pediatric patients with acute Mycoplasma pneumoniae infection |
| title_full | The diagnostic value of serological studies in pediatric patients with acute Mycoplasma pneumoniae infection |
| title_fullStr | The diagnostic value of serological studies in pediatric patients with acute Mycoplasma pneumoniae infection |
| title_full_unstemmed | The diagnostic value of serological studies in pediatric patients with acute Mycoplasma pneumoniae infection |
| title_short | The diagnostic value of serological studies in pediatric patients with acute Mycoplasma pneumoniae infection |
| title_sort | diagnostic value of serological studies in pediatric patients with acute mycoplasma pneumoniae infection |
| url | http://www.sciencedirect.com/science/article/pii/S1684118218300756 |
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