Over-expression of c-SKI inhibits isoprenaline-induced myocardial fibrosis in mice
Objective To investigate the effect of c-SKI on isoprenaline(ISO)-induced cardiac fibrosis in mice. Methods Mice were divided into 4 groups: the control group,the model group,the c-SKI gene infection group (LV-SKI group) and the normal control virus infection group (LV-NC group), with 10 in each....
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-03-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200349.pdf |
| _version_ | 1797365802562748416 |
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| author | ZHANG Ying, XIANG Yan, ZHANG Yue, WANG Juan |
| author_facet | ZHANG Ying, XIANG Yan, ZHANG Yue, WANG Juan |
| author_sort | ZHANG Ying, XIANG Yan, ZHANG Yue, WANG Juan |
| collection | DOAJ |
| description | Objective To investigate the effect of c-SKI on isoprenaline(ISO)-induced cardiac fibrosis in mice. Methods Mice were divided into 4 groups: the control group,the model group,the c-SKI gene infection group (LV-SKI group) and the normal control virus infection group (LV-NC group), with 10 in each. After two weeks, in the model group, isoprenaline(ISO) was injected subcutaneously for 30 d, with the first dose of 5 mg/kg followed by 2.5 mg/kg. In the LV-SKI group and the LV-NC group, c-SKI lentivirus and control virus were injected into the tail vein on days 18, 21, 24, 27 and 30 at a dose of 100 μL, titer was 1×108 TU.HE staining and Masson staining was used to observe the pathological changes of myocardial tissue. ELISA was used to detect type Ⅰ and type Ⅲ collagen in myocardial tissue. Western blot was used to detect the expression of c-SKI, interstitial cell marker vimentin, α SMA,endothelial cell marker CD31 and the expression levels of transcription factors Snail, Twist and Slug. Results Compared with the control group, the expression of c-ski in the model group was time-dependent (P＜0.01). In the model group, the myocardial cells were disordered, the interstitium was significantly increased, and the contents of type Ⅰ and type Ⅲ collagen were increased (P＜0.01). Expressions of vimentin, α-SMA, Snail, Twist and Slug were all up regulated, while CD31 protein expression was down-regulated(P＜0.05). After over-expressing c-SKI, compared with the LV-NC group, the myocardial structure of the mice tended to be neat, the interstitial collagen fibers decreased, and the type Ⅰ and Ⅲ collagen in the myocardial tissue decreased (P＜0.01). The expression of vimentin and belt-sma was down-regulated (P＜0.05), and the expression of CD31 was up-regulated (P＜0.05). Meanwhile, the expression of transcription factors Snail, Twist and Slug was also down-regulated (P＜0.01). Conclusions c-SKI expression is down-regulated during myocardial fibrosis in mice, and over-expression of c-SKI can improve ISO-induced myocardial fibrosis by inhibiting End-MT. |
| first_indexed | 2024-03-08T16:55:10Z |
| format | Article |
| id | doaj.art-a73ca7aa233d47c8a30036c375ec7cba |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:55:10Z |
| publishDate | 2021-03-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-a73ca7aa233d47c8a30036c375ec7cba2024-01-05T03:04:40ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-03-01413364369Over-expression of c-SKI inhibits isoprenaline-induced myocardial fibrosis in miceZHANG Ying, XIANG Yan, ZHANG Yue, WANG Juan0Department of Cardiology, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, ChinaObjective To investigate the effect of c-SKI on isoprenaline(ISO)-induced cardiac fibrosis in mice. Methods Mice were divided into 4 groups: the control group,the model group,the c-SKI gene infection group (LV-SKI group) and the normal control virus infection group (LV-NC group), with 10 in each. After two weeks, in the model group, isoprenaline(ISO) was injected subcutaneously for 30 d, with the first dose of 5 mg/kg followed by 2.5 mg/kg. In the LV-SKI group and the LV-NC group, c-SKI lentivirus and control virus were injected into the tail vein on days 18, 21, 24, 27 and 30 at a dose of 100 μL, titer was 1×108 TU.HE staining and Masson staining was used to observe the pathological changes of myocardial tissue. ELISA was used to detect type Ⅰ and type Ⅲ collagen in myocardial tissue. Western blot was used to detect the expression of c-SKI, interstitial cell marker vimentin, α SMA,endothelial cell marker CD31 and the expression levels of transcription factors Snail, Twist and Slug. Results Compared with the control group, the expression of c-ski in the model group was time-dependent (P＜0.01). In the model group, the myocardial cells were disordered, the interstitium was significantly increased, and the contents of type Ⅰ and type Ⅲ collagen were increased (P＜0.01). Expressions of vimentin, α-SMA, Snail, Twist and Slug were all up regulated, while CD31 protein expression was down-regulated(P＜0.05). After over-expressing c-SKI, compared with the LV-NC group, the myocardial structure of the mice tended to be neat, the interstitial collagen fibers decreased, and the type Ⅰ and Ⅲ collagen in the myocardial tissue decreased (P＜0.01). The expression of vimentin and belt-sma was down-regulated (P＜0.05), and the expression of CD31 was up-regulated (P＜0.05). Meanwhile, the expression of transcription factors Snail, Twist and Slug was also down-regulated (P＜0.01). Conclusions c-SKI expression is down-regulated during myocardial fibrosis in mice, and over-expression of c-SKI can improve ISO-induced myocardial fibrosis by inhibiting End-MT.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200349.pdfc-ski|cardiac fibrosis|endothelial-mesenchymal transition (end-mt)|transcription factor |
| spellingShingle | ZHANG Ying, XIANG Yan, ZHANG Yue, WANG Juan Over-expression of c-SKI inhibits isoprenaline-induced myocardial fibrosis in mice Jichu yixue yu linchuang c-ski|cardiac fibrosis|endothelial-mesenchymal transition (end-mt)|transcription factor |
| title | Over-expression of c-SKI inhibits isoprenaline-induced myocardial fibrosis in mice |
| title_full | Over-expression of c-SKI inhibits isoprenaline-induced myocardial fibrosis in mice |
| title_fullStr | Over-expression of c-SKI inhibits isoprenaline-induced myocardial fibrosis in mice |
| title_full_unstemmed | Over-expression of c-SKI inhibits isoprenaline-induced myocardial fibrosis in mice |
| title_short | Over-expression of c-SKI inhibits isoprenaline-induced myocardial fibrosis in mice |
| title_sort | over expression of c ski inhibits isoprenaline induced myocardial fibrosis in mice |
| topic | c-ski|cardiac fibrosis|endothelial-mesenchymal transition (end-mt)|transcription factor |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200349.pdf |
| work_keys_str_mv | AT zhangyingxiangyanzhangyuewangjuan overexpressionofcskiinhibitsisoprenalineinducedmyocardialfibrosisinmice |