A Portable Biosensor Based on Au Nanoflower Interface Combined with Electrochemical Immunochromatography for POC Detection of Prostate-Specific Antigen
Serum prostate-specific antigen (PSA) is a widely used for the detection of prostate cancer and is considered the most reliable biomarker. However, the currently reported detection methods cannot achieve rapid monitoring. Here, we report a novel electrochemical immunochromatography (EIC) system for...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/12/5/259 |
| _version_ | 1827670059915411456 |
|---|---|
| author | Yanzhi Dou Zhenhua Li Jing Su Shiping Song |
| author_facet | Yanzhi Dou Zhenhua Li Jing Su Shiping Song |
| author_sort | Yanzhi Dou |
| collection | DOAJ |
| description | Serum prostate-specific antigen (PSA) is a widely used for the detection of prostate cancer and is considered the most reliable biomarker. However, the currently reported detection methods cannot achieve rapid monitoring. Here, we report a novel electrochemical immunochromatography (EIC) system for clinically accurate PSA detection. First, we constructed a carbon interface modified with gold nanoflowers (Au NFs) based on screen-printed carbon electrodes (SPCE), which acted as nanostructures with larger specific surface area that increased the number of PSA capture antibodies and can further improve detection signal-to-noise (S/N) ratio. Then, we fabricated detection chips by combining the SPCE/Au NFs with EIC. Under optimized conditions, the proposed biosensor exhibits high accuracy, taking only 15 minutes to complete detection. By measuring the levels of PSA in clinical blood samples, the biosensor can successfully discriminate clinically diagnosed prostate cancer patients from healthy controls. |
| first_indexed | 2024-03-10T03:15:48Z |
| format | Article |
| id | doaj.art-a73dcaf00a8444f587f203a73c93de73 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T03:15:48Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-a73dcaf00a8444f587f203a73c93de732023-11-23T10:14:52ZengMDPI AGBiosensors2079-63742022-04-0112525910.3390/bios12050259A Portable Biosensor Based on Au Nanoflower Interface Combined with Electrochemical Immunochromatography for POC Detection of Prostate-Specific AntigenYanzhi Dou0Zhenhua Li1Jing Su2Shiping Song3Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaDivision of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaState Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, ChinaDivision of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaSerum prostate-specific antigen (PSA) is a widely used for the detection of prostate cancer and is considered the most reliable biomarker. However, the currently reported detection methods cannot achieve rapid monitoring. Here, we report a novel electrochemical immunochromatography (EIC) system for clinically accurate PSA detection. First, we constructed a carbon interface modified with gold nanoflowers (Au NFs) based on screen-printed carbon electrodes (SPCE), which acted as nanostructures with larger specific surface area that increased the number of PSA capture antibodies and can further improve detection signal-to-noise (S/N) ratio. Then, we fabricated detection chips by combining the SPCE/Au NFs with EIC. Under optimized conditions, the proposed biosensor exhibits high accuracy, taking only 15 minutes to complete detection. By measuring the levels of PSA in clinical blood samples, the biosensor can successfully discriminate clinically diagnosed prostate cancer patients from healthy controls.https://www.mdpi.com/2079-6374/12/5/259portable biosensorAu NFs interfaceEICPOC detectionPSA |
| spellingShingle | Yanzhi Dou Zhenhua Li Jing Su Shiping Song A Portable Biosensor Based on Au Nanoflower Interface Combined with Electrochemical Immunochromatography for POC Detection of Prostate-Specific Antigen Biosensors portable biosensor Au NFs interface EIC POC detection PSA |
| title | A Portable Biosensor Based on Au Nanoflower Interface Combined with Electrochemical Immunochromatography for POC Detection of Prostate-Specific Antigen |
| title_full | A Portable Biosensor Based on Au Nanoflower Interface Combined with Electrochemical Immunochromatography for POC Detection of Prostate-Specific Antigen |
| title_fullStr | A Portable Biosensor Based on Au Nanoflower Interface Combined with Electrochemical Immunochromatography for POC Detection of Prostate-Specific Antigen |
| title_full_unstemmed | A Portable Biosensor Based on Au Nanoflower Interface Combined with Electrochemical Immunochromatography for POC Detection of Prostate-Specific Antigen |
| title_short | A Portable Biosensor Based on Au Nanoflower Interface Combined with Electrochemical Immunochromatography for POC Detection of Prostate-Specific Antigen |
| title_sort | portable biosensor based on au nanoflower interface combined with electrochemical immunochromatography for poc detection of prostate specific antigen |
| topic | portable biosensor Au NFs interface EIC POC detection PSA |
| url | https://www.mdpi.com/2079-6374/12/5/259 |
| work_keys_str_mv | AT yanzhidou aportablebiosensorbasedonaunanoflowerinterfacecombinedwithelectrochemicalimmunochromatographyforpocdetectionofprostatespecificantigen AT zhenhuali aportablebiosensorbasedonaunanoflowerinterfacecombinedwithelectrochemicalimmunochromatographyforpocdetectionofprostatespecificantigen AT jingsu aportablebiosensorbasedonaunanoflowerinterfacecombinedwithelectrochemicalimmunochromatographyforpocdetectionofprostatespecificantigen AT shipingsong aportablebiosensorbasedonaunanoflowerinterfacecombinedwithelectrochemicalimmunochromatographyforpocdetectionofprostatespecificantigen AT yanzhidou portablebiosensorbasedonaunanoflowerinterfacecombinedwithelectrochemicalimmunochromatographyforpocdetectionofprostatespecificantigen AT zhenhuali portablebiosensorbasedonaunanoflowerinterfacecombinedwithelectrochemicalimmunochromatographyforpocdetectionofprostatespecificantigen AT jingsu portablebiosensorbasedonaunanoflowerinterfacecombinedwithelectrochemicalimmunochromatographyforpocdetectionofprostatespecificantigen AT shipingsong portablebiosensorbasedonaunanoflowerinterfacecombinedwithelectrochemicalimmunochromatographyforpocdetectionofprostatespecificantigen |