Intravitreal safety profiles of sol-gel mesoporous silica microparticles and the degradation product (Si(OH)4)
Mesoporous silica has attracted significant attention in the drug delivery area; however, impurities can be a source of toxicity. The current study used commercial microparticles produced at large scale in a well-controlled environment. Micrometer sized mesoporous silica particles were acquired thro...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Drug Delivery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/10717544.2020.1760401 |
| _version_ | 1818871803709227008 |
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| author | Yaoyao Sun Kristyn Huffman William R. Freeman Michael J. Sailor Lingyun Cheng |
| author_facet | Yaoyao Sun Kristyn Huffman William R. Freeman Michael J. Sailor Lingyun Cheng |
| author_sort | Yaoyao Sun |
| collection | DOAJ |
| description | Mesoporous silica has attracted significant attention in the drug delivery area; however, impurities can be a source of toxicity. The current study used commercial microparticles produced at large scale in a well-controlled environment. Micrometer sized mesoporous silica particles were acquired through a commercial vendor and pore structures were characterized by SEM. The three silica particle formulations had a diameter of 15 micrometers and three different pore sizes of 10 nm, 30 nm, and 100 nm. The fourth formulation had particle size of 20–40 micrometers with 50 nm pores. Before in vivo tests, an in vitro cytotoxicity test was conducted with silicic acid, derived from the sol-gel particles, on EA.hy926 cells. Low concentration (2.5 µg/mL) of silicic acid showed no cytotoxicity; however, high concentration (25 µg/mL) was cytotoxic. In vivo intravitreal injection demonstrated that 15 um silica particles with 10 nm pore were safe in both rabbit and guinea pig eyes and the particles lasted in the vitreous for longer than two months. Formulations of with larger pores demonstrated variable localized vitreous cloudiness around the sol-gel particle depot and mild inflammatory cells in the aqueous humor. The incidence of reaction trended higher with larger pores (10 nm: 0%, 30 nm: 29%, 50 nm: 71%, 100 nm: 100%, p < .0001, Cochran Armitage Trend Test). Sol-gel mesoporous silica particles have uniform particle sizes and well-defined pores, which is an advantage for implantation via a fine needle. Selected formulations may be used as an intraocular drug delivery system with proper loading and encapsulation. |
| first_indexed | 2024-12-19T12:28:44Z |
| format | Article |
| id | doaj.art-a73e796871304aeba27543cf7d5d6a1e |
| institution | Directory Open Access Journal |
| issn | 1071-7544 1521-0464 |
| language | English |
| last_indexed | 2024-12-19T12:28:44Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Drug Delivery |
| spelling | doaj.art-a73e796871304aeba27543cf7d5d6a1e2022-12-21T20:21:28ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642020-01-0127170371110.1080/10717544.2020.17604011760401Intravitreal safety profiles of sol-gel mesoporous silica microparticles and the degradation product (Si(OH)4)Yaoyao Sun0Kristyn Huffman1William R. Freeman2Michael J. Sailor3Lingyun Cheng4Department of Ophthalmology, Jacobs Retina Center at Shiley Eye Institute, University of CaliforniaDepartment of Ophthalmology, Jacobs Retina Center at Shiley Eye Institute, University of CaliforniaDepartment of Ophthalmology, Jacobs Retina Center at Shiley Eye Institute, University of CaliforniaDepartment of Chemistry and Biochemistry, University of CaliforniaDepartment of Ophthalmology, Jacobs Retina Center at Shiley Eye Institute, University of CaliforniaMesoporous silica has attracted significant attention in the drug delivery area; however, impurities can be a source of toxicity. The current study used commercial microparticles produced at large scale in a well-controlled environment. Micrometer sized mesoporous silica particles were acquired through a commercial vendor and pore structures were characterized by SEM. The three silica particle formulations had a diameter of 15 micrometers and three different pore sizes of 10 nm, 30 nm, and 100 nm. The fourth formulation had particle size of 20–40 micrometers with 50 nm pores. Before in vivo tests, an in vitro cytotoxicity test was conducted with silicic acid, derived from the sol-gel particles, on EA.hy926 cells. Low concentration (2.5 µg/mL) of silicic acid showed no cytotoxicity; however, high concentration (25 µg/mL) was cytotoxic. In vivo intravitreal injection demonstrated that 15 um silica particles with 10 nm pore were safe in both rabbit and guinea pig eyes and the particles lasted in the vitreous for longer than two months. Formulations of with larger pores demonstrated variable localized vitreous cloudiness around the sol-gel particle depot and mild inflammatory cells in the aqueous humor. The incidence of reaction trended higher with larger pores (10 nm: 0%, 30 nm: 29%, 50 nm: 71%, 100 nm: 100%, p < .0001, Cochran Armitage Trend Test). Sol-gel mesoporous silica particles have uniform particle sizes and well-defined pores, which is an advantage for implantation via a fine needle. Selected formulations may be used as an intraocular drug delivery system with proper loading and encapsulation.http://dx.doi.org/10.1080/10717544.2020.1760401intravitreal drug deliverysol-gel mesoporous silicasilicic acid cytotoxicitysilica pore size and ocular toxicityrabbit and guinea pig eyes |
| spellingShingle | Yaoyao Sun Kristyn Huffman William R. Freeman Michael J. Sailor Lingyun Cheng Intravitreal safety profiles of sol-gel mesoporous silica microparticles and the degradation product (Si(OH)4) Drug Delivery intravitreal drug delivery sol-gel mesoporous silica silicic acid cytotoxicity silica pore size and ocular toxicity rabbit and guinea pig eyes |
| title | Intravitreal safety profiles of sol-gel mesoporous silica microparticles and the degradation product (Si(OH)4) |
| title_full | Intravitreal safety profiles of sol-gel mesoporous silica microparticles and the degradation product (Si(OH)4) |
| title_fullStr | Intravitreal safety profiles of sol-gel mesoporous silica microparticles and the degradation product (Si(OH)4) |
| title_full_unstemmed | Intravitreal safety profiles of sol-gel mesoporous silica microparticles and the degradation product (Si(OH)4) |
| title_short | Intravitreal safety profiles of sol-gel mesoporous silica microparticles and the degradation product (Si(OH)4) |
| title_sort | intravitreal safety profiles of sol gel mesoporous silica microparticles and the degradation product si oh 4 |
| topic | intravitreal drug delivery sol-gel mesoporous silica silicic acid cytotoxicity silica pore size and ocular toxicity rabbit and guinea pig eyes |
| url | http://dx.doi.org/10.1080/10717544.2020.1760401 |
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