Quantitative analysis of dendritic branching pattern of large neurons in human cerebellum
Background/Aim. Dentate nucleus (nucleus dentatus) is the most distant of the cerebellar nuclei and the major system for information transfer in the cerebellum. So far, dendritic branches of four different kinds of large neurons of dentate nucleus, have been considered mainly qualitatively with no q...
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| Format: | Article |
| Language: | English |
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Military Health Department, Ministry of Defance, Serbia
2010-01-01
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| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2010/0042-84501009712M.pdf |
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| author | Milošević Nebojša T. Ristanović Dušan Marić Dušica L. Gudović Radmila Krstonošić Bojana |
| author_facet | Milošević Nebojša T. Ristanović Dušan Marić Dušica L. Gudović Radmila Krstonošić Bojana |
| author_sort | Milošević Nebojša T. |
| collection | DOAJ |
| description | Background/Aim. Dentate nucleus (nucleus dentatus) is the most distant of the cerebellar nuclei and the major system for information transfer in the cerebellum. So far, dendritic branches of four different kinds of large neurons of dentate nucleus, have been considered mainly qualitatively with no quantification of their morphological features. The aim of the study was to test the qualitative hypothesis that the human dentate nucleus is composed of various types of the large neurons by quantitative analysis of their dendritic branching patterns. Methods. Series of horizontal sections of the dentate nuclei were taken from 15 adult human brains, free of diagnosed neurological disorders. The 189 Golgi-impregnated images of large neurons were recorded by a digital camera connected to a light microscope. Dendritic branching patterns of digitized neuronal images were analyzed by modified Sholl and fractal analyses. Results. The number of intersections (Nm), critical radius (rc) and fractal dimension (D) of dendritic branching pattern for four types of the large neurons were calculated, statistically evaluated and analyzed. The results show that there is a significant difference between four neuronal types in one morphometric parameter at least. Conclusion. The present study is the first attempt to analyze quantitatively the dendritic branching pattern of neurons from the dentate nucleus in the human. The hypothesis that the four types of the large neurons exist in this part of human cerebellum is successfully supported. |
| first_indexed | 2024-04-13T14:10:02Z |
| format | Article |
| id | doaj.art-a7491acf059445f0a1c165cd8c23367e |
| institution | Directory Open Access Journal |
| issn | 0042-8450 |
| language | English |
| last_indexed | 2024-04-13T14:10:02Z |
| publishDate | 2010-01-01 |
| publisher | Military Health Department, Ministry of Defance, Serbia |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj.art-a7491acf059445f0a1c165cd8c23367e2022-12-22T02:43:48ZengMilitary Health Department, Ministry of Defance, SerbiaVojnosanitetski Pregled0042-84502010-01-0167971271610.2298/VSP1009712MQuantitative analysis of dendritic branching pattern of large neurons in human cerebellumMilošević Nebojša T.Ristanović DušanMarić Dušica L.Gudović RadmilaKrstonošić BojanaBackground/Aim. Dentate nucleus (nucleus dentatus) is the most distant of the cerebellar nuclei and the major system for information transfer in the cerebellum. So far, dendritic branches of four different kinds of large neurons of dentate nucleus, have been considered mainly qualitatively with no quantification of their morphological features. The aim of the study was to test the qualitative hypothesis that the human dentate nucleus is composed of various types of the large neurons by quantitative analysis of their dendritic branching patterns. Methods. Series of horizontal sections of the dentate nuclei were taken from 15 adult human brains, free of diagnosed neurological disorders. The 189 Golgi-impregnated images of large neurons were recorded by a digital camera connected to a light microscope. Dendritic branching patterns of digitized neuronal images were analyzed by modified Sholl and fractal analyses. Results. The number of intersections (Nm), critical radius (rc) and fractal dimension (D) of dendritic branching pattern for four types of the large neurons were calculated, statistically evaluated and analyzed. The results show that there is a significant difference between four neuronal types in one morphometric parameter at least. Conclusion. The present study is the first attempt to analyze quantitatively the dendritic branching pattern of neurons from the dentate nucleus in the human. The hypothesis that the four types of the large neurons exist in this part of human cerebellum is successfully supported.http://www.doiserbia.nb.rs/img/doi/0042-8450/2010/0042-84501009712M.pdfcerebellumcerebellar nucleianatomy, cross-sectionaldendritesneurons |
| spellingShingle | Milošević Nebojša T. Ristanović Dušan Marić Dušica L. Gudović Radmila Krstonošić Bojana Quantitative analysis of dendritic branching pattern of large neurons in human cerebellum Vojnosanitetski Pregled cerebellum cerebellar nuclei anatomy, cross-sectional dendrites neurons |
| title | Quantitative analysis of dendritic branching pattern of large neurons in human cerebellum |
| title_full | Quantitative analysis of dendritic branching pattern of large neurons in human cerebellum |
| title_fullStr | Quantitative analysis of dendritic branching pattern of large neurons in human cerebellum |
| title_full_unstemmed | Quantitative analysis of dendritic branching pattern of large neurons in human cerebellum |
| title_short | Quantitative analysis of dendritic branching pattern of large neurons in human cerebellum |
| title_sort | quantitative analysis of dendritic branching pattern of large neurons in human cerebellum |
| topic | cerebellum cerebellar nuclei anatomy, cross-sectional dendrites neurons |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2010/0042-84501009712M.pdf |
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