A serological biomarker of type I collagen degradation is related to a more severe, high neutrophilic, obese asthma subtype
Abstract Background Asthma is a heterogeneous disease; therefore, biomarkers that can assist in the identification of subtypes and direct therapy are highly desirable. Asthma is a chronic inflammatory disease that leads to changes in the extracellular matrix (ECM) by matrix metalloproteinases (MMPs)...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | Asthma Research and Practice |
| Online Access: | https://doi.org/10.1186/s40733-022-00084-6 |
| _version_ | 1828846952802942976 |
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| author | Sarah Rank Rønnow Jannie Marie Bülow Sand Line Mærsk Staunstrup Thomas Bahmer Michael Wegmann Lars Lunding Janette Burgess Klaus Rabe Grith Lykke Sorensen Oliver Fuchs Erika V. Mutius Gesine Hansen Matthias Volkmar Kopp Morten Karsdal Diana Julie Leeming Markus Weckmann the ALLIANCE Study Group as part of the German Center of Lung Research (DZL) |
| author_facet | Sarah Rank Rønnow Jannie Marie Bülow Sand Line Mærsk Staunstrup Thomas Bahmer Michael Wegmann Lars Lunding Janette Burgess Klaus Rabe Grith Lykke Sorensen Oliver Fuchs Erika V. Mutius Gesine Hansen Matthias Volkmar Kopp Morten Karsdal Diana Julie Leeming Markus Weckmann the ALLIANCE Study Group as part of the German Center of Lung Research (DZL) |
| author_sort | Sarah Rank Rønnow |
| collection | DOAJ |
| description | Abstract Background Asthma is a heterogeneous disease; therefore, biomarkers that can assist in the identification of subtypes and direct therapy are highly desirable. Asthma is a chronic inflammatory disease that leads to changes in the extracellular matrix (ECM) by matrix metalloproteinases (MMPs) degradation causing fragments of type I collagen that is released into circulation. Objective Here, we asked if MMP-generated type I collagen (C1M) was associated with subtypes of asthma. Methods C1M was serologically assessed at baseline in the adult participants of the All Age Asthma study (ALLIANCE) (n = 233), and in The Prospective Epidemiological Risk Factor study (PERF) (n = 283). In addition, C1M was assessed in mice sensitized to ovalbumin (OVA) and challenged with OVA aerosol. C1M was evaluated in mice with and without acute neutrophilic inflammation provoked by poly(cytidylic-inosinic) acid and mice treated with CP17, a peptide inhibiting neutrophil accumulation. Results Serum C1M was significantly increased in asthmatics compared to healthy controls (p = 0.0005). We found the increased C1M levels in asthmatics were related to blood neutrophil and body mass index (BMI) in the ALLIANCE cohort, which was validated in the PERF cohort. When patients were stratified into obese (BMI > 30) asthmatics with high neutrophil levels and uncontrolled asthma, this group had a significant increase in C1M compared to normal-weight (BMI < 25) asthmatics with low neutrophil levels and controlled asthma (p = 0.0277). C1M was significantly elevated in OVA mice with acute neutrophilic inflammation compared to controls (P = 0.0002) and decreased in mice treated with an inhibitor of neutrophil infiltration (p = 0.047). Conclusion & clinical relevance C1M holds the potential to identify a subtype of asthma that relates to severity, obesity, and high neutrophils. These data suggest that C1M is linked to a subtype of overall inflammation, not only derived from the lung. The link between C1M and neutrophils were further validated in in vivo model. Trial registration (ALLIANCE, NCT02419274 ). |
| first_indexed | 2024-12-12T21:59:02Z |
| format | Article |
| id | doaj.art-a7499d84d2254b5da7fba9a595b4937b |
| institution | Directory Open Access Journal |
| issn | 2054-7064 |
| language | English |
| last_indexed | 2024-12-12T21:59:02Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Asthma Research and Practice |
| spelling | doaj.art-a7499d84d2254b5da7fba9a595b4937b2022-12-22T00:10:34ZengBMCAsthma Research and Practice2054-70642022-04-018111110.1186/s40733-022-00084-6A serological biomarker of type I collagen degradation is related to a more severe, high neutrophilic, obese asthma subtypeSarah Rank Rønnow0Jannie Marie Bülow Sand1Line Mærsk Staunstrup2Thomas Bahmer3Michael Wegmann4Lars Lunding5Janette Burgess6Klaus Rabe7Grith Lykke Sorensen8Oliver Fuchs9Erika V. Mutius10Gesine Hansen11Matthias Volkmar Kopp12Morten Karsdal13Diana Julie Leeming14Markus Weckmann15the ALLIANCE Study Group as part of the German Center of Lung Research (DZL)Nordic Bioscience A/SNordic Bioscience A/SNordic Bioscience A/SUniversity of Copenhagen, HealthUniversity of Copenhagen, HealthUniversity of Copenhagen, HealthDivision of Asthma Mouse Model, Priority Area Asthma & Allergy, Leibniz-Center for Medicine and Biosciences BorstelUniversity of Copenhagen, HealthDepartment of Pathology and Medical Biology, Medical Biology Section, University Medical CenterUniversity Childrens Hospital, Inselspital BernDr. von Hauner Children’s Hospital, University Hospital MunichUniversity Childrens Hospital, Department of Pediatric Pneumology, Allergology and Neonatology Hannover Medical SchoolLungenClinic Grosshansdorf GmbHNordic Bioscience A/SNordic Bioscience A/SAirway Research Center North (ARCN), Member of the German Center for Lung Research (DZL)Abstract Background Asthma is a heterogeneous disease; therefore, biomarkers that can assist in the identification of subtypes and direct therapy are highly desirable. Asthma is a chronic inflammatory disease that leads to changes in the extracellular matrix (ECM) by matrix metalloproteinases (MMPs) degradation causing fragments of type I collagen that is released into circulation. Objective Here, we asked if MMP-generated type I collagen (C1M) was associated with subtypes of asthma. Methods C1M was serologically assessed at baseline in the adult participants of the All Age Asthma study (ALLIANCE) (n = 233), and in The Prospective Epidemiological Risk Factor study (PERF) (n = 283). In addition, C1M was assessed in mice sensitized to ovalbumin (OVA) and challenged with OVA aerosol. C1M was evaluated in mice with and without acute neutrophilic inflammation provoked by poly(cytidylic-inosinic) acid and mice treated with CP17, a peptide inhibiting neutrophil accumulation. Results Serum C1M was significantly increased in asthmatics compared to healthy controls (p = 0.0005). We found the increased C1M levels in asthmatics were related to blood neutrophil and body mass index (BMI) in the ALLIANCE cohort, which was validated in the PERF cohort. When patients were stratified into obese (BMI > 30) asthmatics with high neutrophil levels and uncontrolled asthma, this group had a significant increase in C1M compared to normal-weight (BMI < 25) asthmatics with low neutrophil levels and controlled asthma (p = 0.0277). C1M was significantly elevated in OVA mice with acute neutrophilic inflammation compared to controls (P = 0.0002) and decreased in mice treated with an inhibitor of neutrophil infiltration (p = 0.047). Conclusion & clinical relevance C1M holds the potential to identify a subtype of asthma that relates to severity, obesity, and high neutrophils. These data suggest that C1M is linked to a subtype of overall inflammation, not only derived from the lung. The link between C1M and neutrophils were further validated in in vivo model. Trial registration (ALLIANCE, NCT02419274 ).https://doi.org/10.1186/s40733-022-00084-6 |
| spellingShingle | Sarah Rank Rønnow Jannie Marie Bülow Sand Line Mærsk Staunstrup Thomas Bahmer Michael Wegmann Lars Lunding Janette Burgess Klaus Rabe Grith Lykke Sorensen Oliver Fuchs Erika V. Mutius Gesine Hansen Matthias Volkmar Kopp Morten Karsdal Diana Julie Leeming Markus Weckmann the ALLIANCE Study Group as part of the German Center of Lung Research (DZL) A serological biomarker of type I collagen degradation is related to a more severe, high neutrophilic, obese asthma subtype Asthma Research and Practice |
| title | A serological biomarker of type I collagen degradation is related to a more severe, high neutrophilic, obese asthma subtype |
| title_full | A serological biomarker of type I collagen degradation is related to a more severe, high neutrophilic, obese asthma subtype |
| title_fullStr | A serological biomarker of type I collagen degradation is related to a more severe, high neutrophilic, obese asthma subtype |
| title_full_unstemmed | A serological biomarker of type I collagen degradation is related to a more severe, high neutrophilic, obese asthma subtype |
| title_short | A serological biomarker of type I collagen degradation is related to a more severe, high neutrophilic, obese asthma subtype |
| title_sort | serological biomarker of type i collagen degradation is related to a more severe high neutrophilic obese asthma subtype |
| url | https://doi.org/10.1186/s40733-022-00084-6 |
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