Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus)
In this study, we describe the immunohistochemical characterisation of the thymus, the main lymphoid organ, in sturgeon. The wide range cytokeratin, vimentin, S-100 protein, LCA (CD45) and CD3 were selected as the immunohistochemical markers to map the thymus in juvenile sterlet (Acipenser ruthenus)...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2020-07-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-202007-0003_immunohistochemical-mapping-of-x202f-thymic-microenvironment-in-x202f-sterlet-acipenser-ruthenus.php |
| _version_ | 1797897459155861504 |
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| author | Eva Salkova Martin Flajshans Christoph Steinbach |
| author_facet | Eva Salkova Martin Flajshans Christoph Steinbach |
| author_sort | Eva Salkova |
| collection | DOAJ |
| description | In this study, we describe the immunohistochemical characterisation of the thymus, the main lymphoid organ, in sturgeon. The wide range cytokeratin, vimentin, S-100 protein, LCA (CD45) and CD3 were selected as the immunohistochemical markers to map the thymus in juvenile sterlet (Acipenser ruthenus). The epithelial cells and Hassall's corpuscles were labelled with a wide range cytokeratin. The fibroblasts and connective tissue within the thin fibrous capsule on the thymic surface expressed vimentin positivity. The stromal reticular cells were S-100 protein positive. The Leukocyte Common Antigen LCA (CD45) was negative on the thymic lymphocytes. The CD3 was negative on the thymic lymphocytes with cross-reactivity on the non-targeted structures. In conclusion, the commercially available antibodies against the wide range cytokeratin, vimentin and S-100 protein can be used to differentiate components of the sturgeon thymus, while the LCA (CD45) and CD3 application failed. We suggest that further studies are needed to generate fish specific antibodies. |
| first_indexed | 2024-04-10T07:58:50Z |
| format | Article |
| id | doaj.art-a749bbd913fe4dec8e25f5096478df6e |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:58:50Z |
| publishDate | 2020-07-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-a749bbd913fe4dec8e25f5096478df6e2023-02-23T03:50:59ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922020-07-0165730130810.17221/181/2019-VETMEDvet-202007-0003Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus)Eva SalkovaMartin Flajshans0Christoph Steinbach1South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech RepublicSouth Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech RepublicIn this study, we describe the immunohistochemical characterisation of the thymus, the main lymphoid organ, in sturgeon. The wide range cytokeratin, vimentin, S-100 protein, LCA (CD45) and CD3 were selected as the immunohistochemical markers to map the thymus in juvenile sterlet (Acipenser ruthenus). The epithelial cells and Hassall's corpuscles were labelled with a wide range cytokeratin. The fibroblasts and connective tissue within the thin fibrous capsule on the thymic surface expressed vimentin positivity. The stromal reticular cells were S-100 protein positive. The Leukocyte Common Antigen LCA (CD45) was negative on the thymic lymphocytes. The CD3 was negative on the thymic lymphocytes with cross-reactivity on the non-targeted structures. In conclusion, the commercially available antibodies against the wide range cytokeratin, vimentin and S-100 protein can be used to differentiate components of the sturgeon thymus, while the LCA (CD45) and CD3 application failed. We suggest that further studies are needed to generate fish specific antibodies.https://vetmed.agriculturejournals.cz/artkey/vet-202007-0003_immunohistochemical-mapping-of-x202f-thymic-microenvironment-in-x202f-sterlet-acipenser-ruthenus.phpcd3hassall's corpuscleslca (cd45)s-100 proteinvimentinwide range cytokeratin |
| spellingShingle | Eva Salkova Martin Flajshans Christoph Steinbach Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus) Veterinární Medicína cd3 hassall's corpuscles lca (cd45) s-100 protein vimentin wide range cytokeratin |
| title | Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus) |
| title_full | Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus) |
| title_fullStr | Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus) |
| title_full_unstemmed | Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus) |
| title_short | Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus) |
| title_sort | immunohistochemical mapping of thymic microenvironment in sterlet acipenser ruthenus |
| topic | cd3 hassall's corpuscles lca (cd45) s-100 protein vimentin wide range cytokeratin |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-202007-0003_immunohistochemical-mapping-of-x202f-thymic-microenvironment-in-x202f-sterlet-acipenser-ruthenus.php |
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