Megakaryocyte apoptosis in immune thrombocytopenia
The mechanisms of platelet underproduction in immune thrombocytopenia (ITP) remain unknown. While the number of megakaryocytes is normal or increased in ITP bone marrow, further studies of megakaryocyte integrity are needed. Megakaryocytes are responsible for the production of platelets in the bone...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2018-10-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2018.1475637 |
| _version_ | 1797684285318103040 |
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| author | John R. Vrbensky Ishac Nazy Lisa J. Toltl Catherine Ross Nikola Ivetic James W. Smith John G. Kelton Donald M. Arnold |
| author_facet | John R. Vrbensky Ishac Nazy Lisa J. Toltl Catherine Ross Nikola Ivetic James W. Smith John G. Kelton Donald M. Arnold |
| author_sort | John R. Vrbensky |
| collection | DOAJ |
| description | The mechanisms of platelet underproduction in immune thrombocytopenia (ITP) remain unknown. While the number of megakaryocytes is normal or increased in ITP bone marrow, further studies of megakaryocyte integrity are needed. Megakaryocytes are responsible for the production of platelets in the bone marrow, and they are possible targets of immune-mediated injury in ITP. Since the biological process of megakaryocyte apoptosis impacts platelet production, we investigated megakaryocyte DNA fragmentation as a marker of apoptosis from ITP bone marrow biopsies. Archived bone marrow biopsy specimens from ITP patients, bone marrow specimens from controls with normal platelet counts, and bone marrow specimens from thrombocytopenic controls with myelodysplastic syndrome (MDS) were evaluated. Sections were stained with anti-CD61 for megakaryocyte enumeration, and terminal deoxynucleotidyl transferase dUTP nick-end labeling was used as an apoptotic indicator. In ITP patients, megakaryocyte apoptosis was reduced compared to nonthrombocytopenic controls. Megakaryocyte apoptosis was similarly reduced in thrombocytopenic patients with MDS. These results suggest a link between megakaryocyte apoptosis and platelet production. |
| first_indexed | 2024-03-12T00:27:14Z |
| format | Article |
| id | doaj.art-a749e5bfce2549b88b7dcafe69e1c049 |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:27:14Z |
| publishDate | 2018-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-a749e5bfce2549b88b7dcafe69e1c0492023-09-15T10:31:59ZengTaylor & Francis GroupPlatelets0953-71041369-16352018-10-0129772973210.1080/09537104.2018.14756371475637Megakaryocyte apoptosis in immune thrombocytopeniaJohn R. Vrbensky0Ishac Nazy1Lisa J. Toltl2Catherine Ross3Nikola Ivetic4James W. Smith5John G. Kelton6Donald M. Arnold7McMaster UniversityMcMaster UniversityMcMaster UniversityMcMaster UniversityMcMaster UniversityMcMaster UniversityMcMaster UniversityMcMaster UniversityThe mechanisms of platelet underproduction in immune thrombocytopenia (ITP) remain unknown. While the number of megakaryocytes is normal or increased in ITP bone marrow, further studies of megakaryocyte integrity are needed. Megakaryocytes are responsible for the production of platelets in the bone marrow, and they are possible targets of immune-mediated injury in ITP. Since the biological process of megakaryocyte apoptosis impacts platelet production, we investigated megakaryocyte DNA fragmentation as a marker of apoptosis from ITP bone marrow biopsies. Archived bone marrow biopsy specimens from ITP patients, bone marrow specimens from controls with normal platelet counts, and bone marrow specimens from thrombocytopenic controls with myelodysplastic syndrome (MDS) were evaluated. Sections were stained with anti-CD61 for megakaryocyte enumeration, and terminal deoxynucleotidyl transferase dUTP nick-end labeling was used as an apoptotic indicator. In ITP patients, megakaryocyte apoptosis was reduced compared to nonthrombocytopenic controls. Megakaryocyte apoptosis was similarly reduced in thrombocytopenic patients with MDS. These results suggest a link between megakaryocyte apoptosis and platelet production.http://dx.doi.org/10.1080/09537104.2018.1475637apoptosisautoimmune diseasebone marrow pathologyitpmegakaryocytes |
| spellingShingle | John R. Vrbensky Ishac Nazy Lisa J. Toltl Catherine Ross Nikola Ivetic James W. Smith John G. Kelton Donald M. Arnold Megakaryocyte apoptosis in immune thrombocytopenia Platelets apoptosis autoimmune disease bone marrow pathology itp megakaryocytes |
| title | Megakaryocyte apoptosis in immune thrombocytopenia |
| title_full | Megakaryocyte apoptosis in immune thrombocytopenia |
| title_fullStr | Megakaryocyte apoptosis in immune thrombocytopenia |
| title_full_unstemmed | Megakaryocyte apoptosis in immune thrombocytopenia |
| title_short | Megakaryocyte apoptosis in immune thrombocytopenia |
| title_sort | megakaryocyte apoptosis in immune thrombocytopenia |
| topic | apoptosis autoimmune disease bone marrow pathology itp megakaryocytes |
| url | http://dx.doi.org/10.1080/09537104.2018.1475637 |
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