Single-Shot 3D Topography of Transmissive and Reflective Samples with a Dual-Mode Telecentric-Based Digital Holographic Microscope
Common path DHM systems are the most robust DHM systems as they are based on self-interference and are thus less prone to external fluctuations. A common issue amongst these DHM systems is that the two replicas of the sample’s information overlay due to self-interference, making them only suitable f...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/22/10/3793 |
| _version_ | 1797495686604783616 |
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| author | Ana Doblas Charity Hayes-Rounds Rohan Isaac Felio Perez |
| author_facet | Ana Doblas Charity Hayes-Rounds Rohan Isaac Felio Perez |
| author_sort | Ana Doblas |
| collection | DOAJ |
| description | Common path DHM systems are the most robust DHM systems as they are based on self-interference and are thus less prone to external fluctuations. A common issue amongst these DHM systems is that the two replicas of the sample’s information overlay due to self-interference, making them only suitable for imaging sparse samples. This overlay has restricted the use of common-path DHM systems in material science. The overlay can be overcome by limiting the sample’s field of view to occupy only half of the imaging field of view or by using an optical spatial filter. In this work, we have implemented optical spatial filtering in a common-path DHM system using a Fresnel biprism. We have analyzed the optimal pinhole size by evaluating the frequency content of the reconstructed phase images of a star target. We have also measured the accuracy of the system and the sensitivity to noise for different pinhole sizes. Finally, we have proposed the first dual-mode common-path DHM system using a Fresnel biprism. The performance of the dual-model DHM system has been evaluated experimentally using transmissive and reflective microscopic samples. |
| first_indexed | 2024-03-10T01:53:12Z |
| format | Article |
| id | doaj.art-a74aafaf9d2340e6b132041fb7659b37 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T01:53:12Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-a74aafaf9d2340e6b132041fb7659b372023-11-23T13:01:19ZengMDPI AGSensors1424-82202022-05-012210379310.3390/s22103793Single-Shot 3D Topography of Transmissive and Reflective Samples with a Dual-Mode Telecentric-Based Digital Holographic MicroscopeAna Doblas0Charity Hayes-Rounds1Rohan Isaac2Felio Perez3Department of Electrical and Computer Engineering, The University of Memphis, Memphis, TN 38152, USADepartment of Electrical and Computer Engineering, The University of Memphis, Memphis, TN 38152, USAFedEx Institute of Technology, The University of Memphis, Memphis, TN 38152, USAMaterial Science Laboratory, Integrated Microscopy Center, The University of Memphis, Memphis, TN 38152, USACommon path DHM systems are the most robust DHM systems as they are based on self-interference and are thus less prone to external fluctuations. A common issue amongst these DHM systems is that the two replicas of the sample’s information overlay due to self-interference, making them only suitable for imaging sparse samples. This overlay has restricted the use of common-path DHM systems in material science. The overlay can be overcome by limiting the sample’s field of view to occupy only half of the imaging field of view or by using an optical spatial filter. In this work, we have implemented optical spatial filtering in a common-path DHM system using a Fresnel biprism. We have analyzed the optimal pinhole size by evaluating the frequency content of the reconstructed phase images of a star target. We have also measured the accuracy of the system and the sensitivity to noise for different pinhole sizes. Finally, we have proposed the first dual-mode common-path DHM system using a Fresnel biprism. The performance of the dual-model DHM system has been evaluated experimentally using transmissive and reflective microscopic samples.https://www.mdpi.com/1424-8220/22/10/3793common-path interferometrydigital holographic microscopyFresnel biprism |
| spellingShingle | Ana Doblas Charity Hayes-Rounds Rohan Isaac Felio Perez Single-Shot 3D Topography of Transmissive and Reflective Samples with a Dual-Mode Telecentric-Based Digital Holographic Microscope Sensors common-path interferometry digital holographic microscopy Fresnel biprism |
| title | Single-Shot 3D Topography of Transmissive and Reflective Samples with a Dual-Mode Telecentric-Based Digital Holographic Microscope |
| title_full | Single-Shot 3D Topography of Transmissive and Reflective Samples with a Dual-Mode Telecentric-Based Digital Holographic Microscope |
| title_fullStr | Single-Shot 3D Topography of Transmissive and Reflective Samples with a Dual-Mode Telecentric-Based Digital Holographic Microscope |
| title_full_unstemmed | Single-Shot 3D Topography of Transmissive and Reflective Samples with a Dual-Mode Telecentric-Based Digital Holographic Microscope |
| title_short | Single-Shot 3D Topography of Transmissive and Reflective Samples with a Dual-Mode Telecentric-Based Digital Holographic Microscope |
| title_sort | single shot 3d topography of transmissive and reflective samples with a dual mode telecentric based digital holographic microscope |
| topic | common-path interferometry digital holographic microscopy Fresnel biprism |
| url | https://www.mdpi.com/1424-8220/22/10/3793 |
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