Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome
Aim: We aimed to examine the alterations of the insulin signaling pathway, autophagy, nitrative stress and the effect of vitamin D supplementation in the liver and ovaries of vitamin D deficient hyperandrogenic rats. Methods: Female Wistar rats received eight weeks of transdermal testosterone treatm...
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| Format: | Article |
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MDPI AG
2019-09-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/9/471 |
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| author | Krisztina Lajtai Csilla Terézia Nagy Róbert Tarszabó Rita Benkő Leila Hadjadj Réka Eszter Sziva Dóra Gerszi Bálint Bányai Péter Ferdinandy György László Nádasy Zoltán Giricz Eszter Mária Horváth Szabolcs Várbíró |
| author_facet | Krisztina Lajtai Csilla Terézia Nagy Róbert Tarszabó Rita Benkő Leila Hadjadj Réka Eszter Sziva Dóra Gerszi Bálint Bányai Péter Ferdinandy György László Nádasy Zoltán Giricz Eszter Mária Horváth Szabolcs Várbíró |
| author_sort | Krisztina Lajtai |
| collection | DOAJ |
| description | Aim: We aimed to examine the alterations of the insulin signaling pathway, autophagy, nitrative stress and the effect of vitamin D supplementation in the liver and ovaries of vitamin D deficient hyperandrogenic rats. Methods: Female Wistar rats received eight weeks of transdermal testosterone treatment and lived on a low vitamin D diet (D−T+). Vitamin D supplementation was achieved by oral administration of vitamin D3 (D+T+). Sham-treated (D+T−) and vitamin D deficient animals (D−T−) served as controls. (N = 10−12 per group). Results: D−T+ animals showed decreased LC3 II levels in the liver and increased p-Akt/Akt and p-eNOS/eNOS ratios with decreased insulin receptor staining in the ovaries. Vitamin D supplementation prevented the increase of Akt phosphorylation in the ovaries. Vitamin D deficiency itself also led to decreased LC3 II levels in the liver and decreased insulin receptor staining in the ovaries. D−T+ group showed no increase in nitrotyrosine staining; however, the ovaries of D−T− rats and the liver of D+T+ animals showed increased staining intensity. Conclusion: Vitamin D deficiency itself might lead to disrupted ovarian maturation and autophagy malfunction in the liver. Preventing Akt phosphorylation may contribute to the beneficial effect of vitamin D treatment on ovarian function in hyperandrogenism. |
| first_indexed | 2024-04-12T19:36:38Z |
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| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-04-12T19:36:38Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-a74e9527bb2b49e7a5069e8f36de9d922022-12-22T03:19:11ZengMDPI AGBiomolecules2218-273X2019-09-019947110.3390/biom9090471biom9090471Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary SyndromeKrisztina Lajtai0Csilla Terézia Nagy1Róbert Tarszabó2Rita Benkő3Leila Hadjadj4Réka Eszter Sziva5Dóra Gerszi6Bálint Bányai7Péter Ferdinandy8György László Nádasy9Zoltán Giricz10Eszter Mária Horváth11Szabolcs Várbíró12Department of Obstetrics and Gynecology, Semmelweis University, Üllői út 78/a, 1082 Budapest, HungaryDepartment of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, HungaryDepartment of Physiology, Semmelweis University, Tűzoltó utca 37-47, 1094 Budapest, HungaryDepartment of Physiology, Semmelweis University, Tűzoltó utca 37-47, 1094 Budapest, HungaryInstitute of Clinical Experimental Research, Semmelweis University, Tűzoltó utca 37-47, 1094 Budapest, HungaryDepartment of Obstetrics and Gynecology, Semmelweis University, Üllői út 78/a, 1082 Budapest, HungaryDepartment of Obstetrics and Gynecology, Semmelweis University, Üllői út 78/a, 1082 Budapest, HungaryDepartment of Physiology, Semmelweis University, Tűzoltó utca 37-47, 1094 Budapest, HungaryDepartment of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, HungaryDepartment of Physiology, Semmelweis University, Tűzoltó utca 37-47, 1094 Budapest, HungaryDepartment of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, HungaryDepartment of Physiology, Semmelweis University, Tűzoltó utca 37-47, 1094 Budapest, HungaryDepartment of Obstetrics and Gynecology, Semmelweis University, Üllői út 78/a, 1082 Budapest, HungaryAim: We aimed to examine the alterations of the insulin signaling pathway, autophagy, nitrative stress and the effect of vitamin D supplementation in the liver and ovaries of vitamin D deficient hyperandrogenic rats. Methods: Female Wistar rats received eight weeks of transdermal testosterone treatment and lived on a low vitamin D diet (D−T+). Vitamin D supplementation was achieved by oral administration of vitamin D3 (D+T+). Sham-treated (D+T−) and vitamin D deficient animals (D−T−) served as controls. (N = 10−12 per group). Results: D−T+ animals showed decreased LC3 II levels in the liver and increased p-Akt/Akt and p-eNOS/eNOS ratios with decreased insulin receptor staining in the ovaries. Vitamin D supplementation prevented the increase of Akt phosphorylation in the ovaries. Vitamin D deficiency itself also led to decreased LC3 II levels in the liver and decreased insulin receptor staining in the ovaries. D−T+ group showed no increase in nitrotyrosine staining; however, the ovaries of D−T− rats and the liver of D+T+ animals showed increased staining intensity. Conclusion: Vitamin D deficiency itself might lead to disrupted ovarian maturation and autophagy malfunction in the liver. Preventing Akt phosphorylation may contribute to the beneficial effect of vitamin D treatment on ovarian function in hyperandrogenism.https://www.mdpi.com/2218-273X/9/9/471hyperandrogenismvitamin Dpolycystic ovary syndrome (PCOS)insulin resistanceautophagyoxidative stress |
| spellingShingle | Krisztina Lajtai Csilla Terézia Nagy Róbert Tarszabó Rita Benkő Leila Hadjadj Réka Eszter Sziva Dóra Gerszi Bálint Bányai Péter Ferdinandy György László Nádasy Zoltán Giricz Eszter Mária Horváth Szabolcs Várbíró Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome Biomolecules hyperandrogenism vitamin D polycystic ovary syndrome (PCOS) insulin resistance autophagy oxidative stress |
| title | Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome |
| title_full | Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome |
| title_fullStr | Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome |
| title_full_unstemmed | Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome |
| title_short | Effects of Vitamin D Deficiency on Proliferation and Autophagy of Ovarian and Liver Tissues in a Rat Model of Polycystic Ovary Syndrome |
| title_sort | effects of vitamin d deficiency on proliferation and autophagy of ovarian and liver tissues in a rat model of polycystic ovary syndrome |
| topic | hyperandrogenism vitamin D polycystic ovary syndrome (PCOS) insulin resistance autophagy oxidative stress |
| url | https://www.mdpi.com/2218-273X/9/9/471 |
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