Cytotoxic Activity of Familact: A Probiotic Supplement
Background: Lactobacillus and Bifidobacterium species are among the probiotics discussed due to their anti-cancer effects in the treatment of colorectal and breast cancers in recent studies. The aim of this study was to investigate the anticancer effect of Familact, a commercial probiotic capsule co...
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| Format: | Article |
| Language: | English |
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Arak University of Medical Sciences
2018-07-01
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| Series: | Iranian Journal of Toxicology |
| Subjects: | |
| Online Access: | http://ijt.arakmu.ac.ir/article-1-694-en.pdf |
| _version_ | 1811266603855118336 |
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| author | Zahra Yahyavi Mohammad Reza Fazeli Mani Mirfeizi Shima Aliebrahimi Seyed Nasser Ostad* |
| author_facet | Zahra Yahyavi Mohammad Reza Fazeli Mani Mirfeizi Shima Aliebrahimi Seyed Nasser Ostad* |
| author_sort | Zahra Yahyavi |
| collection | DOAJ |
| description | Background: Lactobacillus and Bifidobacterium species are among the probiotics discussed due to their anti-cancer effects in the treatment of colorectal and breast cancers in recent studies. The aim of this study was to investigate the anticancer effect of Familact, a commercial probiotic capsule containing seven bacterial strains (L. casei, L. acidophilus, L. rhamnosus, L. bulgaricus, B. breve, B. longum and Streptococcus thermophilus).
Methods: Various cancer cell lines including Caco-2, HT-29, T47D and normal cell line L929 were treated with different concentrations of Familact. Using MTT assay, the cytotoxicity effect was investigated for each cell line and then flow cytometry analysis of apoptosis was evaluated.
Results: Familact demonstrated inhibitory effects on the proliferation of all tested cancer cell lines in a dose-dependent manner. Although Familact augmented apoptotic cell death in HT-29 human cancer cells, it was less effective in the case of Caco-2 and T47D cells. Moreover, exposure to Familact showed moderate cytotoxicity towards L929 mouse fibroblast cells.
Conclusion: Familact could be considered as a complementary therapy in the treatment of cancers. |
| first_indexed | 2024-04-12T20:46:10Z |
| format | Article |
| id | doaj.art-a75007ada04c4ea48777b9cbf1ce735e |
| institution | Directory Open Access Journal |
| issn | 2008-2967 2251-9459 |
| language | English |
| last_indexed | 2024-04-12T20:46:10Z |
| publishDate | 2018-07-01 |
| publisher | Arak University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Toxicology |
| spelling | doaj.art-a75007ada04c4ea48777b9cbf1ce735e2022-12-22T03:17:16ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592018-07-011244145Cytotoxic Activity of Familact: A Probiotic SupplementZahra Yahyavi 0Mohammad Reza Fazeli1Mani Mirfeizi2Shima Aliebrahimi3Seyed Nasser Ostad*4Department of Microbiology, Faculty of Basic Sciences, Karaj Branch, Islamic Azad University, Alborz, Iran. Department of Drug & Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.Department of Midwifery, College of Nursing and Midwifery, Karaj Branch, Islamic Azad University, Karaj, Iran.Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.*Corresponding Author: E-mail: ostadnas@tums.ac.irBackground: Lactobacillus and Bifidobacterium species are among the probiotics discussed due to their anti-cancer effects in the treatment of colorectal and breast cancers in recent studies. The aim of this study was to investigate the anticancer effect of Familact, a commercial probiotic capsule containing seven bacterial strains (L. casei, L. acidophilus, L. rhamnosus, L. bulgaricus, B. breve, B. longum and Streptococcus thermophilus). Methods: Various cancer cell lines including Caco-2, HT-29, T47D and normal cell line L929 were treated with different concentrations of Familact. Using MTT assay, the cytotoxicity effect was investigated for each cell line and then flow cytometry analysis of apoptosis was evaluated. Results: Familact demonstrated inhibitory effects on the proliferation of all tested cancer cell lines in a dose-dependent manner. Although Familact augmented apoptotic cell death in HT-29 human cancer cells, it was less effective in the case of Caco-2 and T47D cells. Moreover, exposure to Familact showed moderate cytotoxicity towards L929 mouse fibroblast cells. Conclusion: Familact could be considered as a complementary therapy in the treatment of cancers.http://ijt.arakmu.ac.ir/article-1-694-en.pdfApoptosisBreast CancerColorectal CancerFamilactPrebioticProbiotics |
| spellingShingle | Zahra Yahyavi Mohammad Reza Fazeli Mani Mirfeizi Shima Aliebrahimi Seyed Nasser Ostad* Cytotoxic Activity of Familact: A Probiotic Supplement Iranian Journal of Toxicology Apoptosis Breast Cancer Colorectal Cancer Familact Prebiotic Probiotics |
| title | Cytotoxic Activity of Familact: A Probiotic Supplement |
| title_full | Cytotoxic Activity of Familact: A Probiotic Supplement |
| title_fullStr | Cytotoxic Activity of Familact: A Probiotic Supplement |
| title_full_unstemmed | Cytotoxic Activity of Familact: A Probiotic Supplement |
| title_short | Cytotoxic Activity of Familact: A Probiotic Supplement |
| title_sort | cytotoxic activity of familact a probiotic supplement |
| topic | Apoptosis Breast Cancer Colorectal Cancer Familact Prebiotic Probiotics |
| url | http://ijt.arakmu.ac.ir/article-1-694-en.pdf |
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