p53-Dependent transcriptional responses to interleukin-3 signaling.
p53 is critical in the normal response to a variety of cellular stresses including DNA damage and loss of p53 function is a common feature of many cancers. In hematological malignancies, p53 deletion is less common than in solid malignancies but is associated with poor prognosis and resistance to ch...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3279357?pdf=render |
| _version_ | 1818947949485359104 |
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| author | Anissa M Jabbour Lavinia Gordon Carmel P Daunt Benjamin D Green Chung H Kok Richard D'Andrea Paul G Ekert |
| author_facet | Anissa M Jabbour Lavinia Gordon Carmel P Daunt Benjamin D Green Chung H Kok Richard D'Andrea Paul G Ekert |
| author_sort | Anissa M Jabbour |
| collection | DOAJ |
| description | p53 is critical in the normal response to a variety of cellular stresses including DNA damage and loss of p53 function is a common feature of many cancers. In hematological malignancies, p53 deletion is less common than in solid malignancies but is associated with poor prognosis and resistance to chemotherapy. Compared to their wild-type (WT) counterparts, hematopoietic progenitor cells lacking p53 have a greater propensity to survive cytokine loss, in part, due to the failure to transcribe Puma, a proapoptotic Bcl-2 family member. Using expression arrays, we have further characterized the differences that distinguish p53(-/-) cells from WT myeloid cells in the presence of Interleukin-3 (IL-3) to determine if such differences contribute to the increased clonogenicity and survival responses observed in p53(-/-) cells. We show that p53(-/-) cells have a deregulated intracellular signaling environment and display a more rapid and sustained response to IL-3. This was accompanied by an increase in active ERK1/2 and a dependence on an intact MAP kinase signaling pathway. Contrastingly, we find that p53(-/-) cells are independent on AKT for their survival. Thus, loss of p53 in myeloid cells results in an altered transcriptional and kinase signaling environment that favors enhanced cytokine signaling. |
| first_indexed | 2024-12-20T08:39:02Z |
| format | Article |
| id | doaj.art-a750fb97acc74931ad4395ca8d2afb30 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T08:39:02Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a750fb97acc74931ad4395ca8d2afb302022-12-21T19:46:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0172e3142810.1371/journal.pone.0031428p53-Dependent transcriptional responses to interleukin-3 signaling.Anissa M JabbourLavinia GordonCarmel P DauntBenjamin D GreenChung H KokRichard D'AndreaPaul G Ekertp53 is critical in the normal response to a variety of cellular stresses including DNA damage and loss of p53 function is a common feature of many cancers. In hematological malignancies, p53 deletion is less common than in solid malignancies but is associated with poor prognosis and resistance to chemotherapy. Compared to their wild-type (WT) counterparts, hematopoietic progenitor cells lacking p53 have a greater propensity to survive cytokine loss, in part, due to the failure to transcribe Puma, a proapoptotic Bcl-2 family member. Using expression arrays, we have further characterized the differences that distinguish p53(-/-) cells from WT myeloid cells in the presence of Interleukin-3 (IL-3) to determine if such differences contribute to the increased clonogenicity and survival responses observed in p53(-/-) cells. We show that p53(-/-) cells have a deregulated intracellular signaling environment and display a more rapid and sustained response to IL-3. This was accompanied by an increase in active ERK1/2 and a dependence on an intact MAP kinase signaling pathway. Contrastingly, we find that p53(-/-) cells are independent on AKT for their survival. Thus, loss of p53 in myeloid cells results in an altered transcriptional and kinase signaling environment that favors enhanced cytokine signaling.http://europepmc.org/articles/PMC3279357?pdf=render |
| spellingShingle | Anissa M Jabbour Lavinia Gordon Carmel P Daunt Benjamin D Green Chung H Kok Richard D'Andrea Paul G Ekert p53-Dependent transcriptional responses to interleukin-3 signaling. PLoS ONE |
| title | p53-Dependent transcriptional responses to interleukin-3 signaling. |
| title_full | p53-Dependent transcriptional responses to interleukin-3 signaling. |
| title_fullStr | p53-Dependent transcriptional responses to interleukin-3 signaling. |
| title_full_unstemmed | p53-Dependent transcriptional responses to interleukin-3 signaling. |
| title_short | p53-Dependent transcriptional responses to interleukin-3 signaling. |
| title_sort | p53 dependent transcriptional responses to interleukin 3 signaling |
| url | http://europepmc.org/articles/PMC3279357?pdf=render |
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