Oleanolic Acid-Enriched Olive Oil Alleviates the Interleukin-6 Overproduction Induced by Postprandial Triglyceride-Rich Lipoproteins in THP-1 Macrophages
Oleanolic acid (OA), a triterpene that is highly present in olive leaves, has been proposed as a component of functional foods for the prevention of metabolic syndrome, due to its anti-inflammatory activity. We analyzed the effects of OA on inflammatory parameters and signaling proteins in LPS-stimu...
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MDPI AG
2021-09-01
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| Series: | Nutrients |
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| author | Ángel Fernández-Aparicio Javier S. Perona José M. Castellano María Correa-Rodríguez Jacqueline Schmidt-RioValle Emilio González-Jiménez |
| author_facet | Ángel Fernández-Aparicio Javier S. Perona José M. Castellano María Correa-Rodríguez Jacqueline Schmidt-RioValle Emilio González-Jiménez |
| author_sort | Ángel Fernández-Aparicio |
| collection | DOAJ |
| description | Oleanolic acid (OA), a triterpene that is highly present in olive leaves, has been proposed as a component of functional foods for the prevention of metabolic syndrome, due to its anti-inflammatory activity. We analyzed the effects of OA on inflammatory parameters and signaling proteins in LPS-stimulated THP-1 macrophages. Thus, THP-1 macrophages were incubated with LPS for 48 h after pretreatment with OA at different concentrations. Pretreatment with OA was significantly effective in attenuating IL-6 and TNF-α overproduction induced by LPS in macrophages, and also improved the levels of AMPK-α. We also evaluated the effects of human triglyceride-rich lipoproteins (TRLs) derived from individuals consuming an OA-enriched functional olive oil. For this purpose, TRLs were isolated from healthy adolescents before, 2 and 5 h postprandially after the intake of a meal containing the functional olive oil or common olive oil, and were incubated with THP-1 macrophages. THP-1 macrophages incubated with TRLs isolated at 2 h after the consumption of the OA-enriched olive oil showed significant lower levels of IL-6 compared to the TRLs derived from olive oil. Our results suggest that OA might have potential to be used as a lipid-based formulation in functional olive oils to prevent inflammatory processes underlying metabolic syndrome in adolescents. |
| first_indexed | 2024-03-10T06:18:26Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T06:18:26Z |
| publishDate | 2021-09-01 |
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| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-a7525ec631d24cf1a425a5082c169b562023-11-22T19:29:04ZengMDPI AGNutrients2072-66432021-09-011310347110.3390/nu13103471Oleanolic Acid-Enriched Olive Oil Alleviates the Interleukin-6 Overproduction Induced by Postprandial Triglyceride-Rich Lipoproteins in THP-1 MacrophagesÁngel Fernández-Aparicio0Javier S. Perona1José M. Castellano2María Correa-Rodríguez3Jacqueline Schmidt-RioValle4Emilio González-Jiménez5Department of Nursing, Faculty of Health Sciences, University of Granada, Av. Ilustración, 60, 18016 Granada, SpainDepartment of Food and Health, Instituto de la Grasa-CSIC, Campus of the University Pablo de Olavide, Building 46, 41013 Seville, SpainDepartment of Food and Health, Instituto de la Grasa-CSIC, Campus of the University Pablo de Olavide, Building 46, 41013 Seville, SpainDepartment of Nursing, Faculty of Health Sciences, University of Granada, Av. Ilustración, 60, 18016 Granada, SpainDepartment of Nursing, Faculty of Health Sciences, University of Granada, Av. Ilustración, 60, 18016 Granada, SpainDepartment of Nursing, Faculty of Health Sciences, University of Granada, Av. Ilustración, 60, 18016 Granada, SpainOleanolic acid (OA), a triterpene that is highly present in olive leaves, has been proposed as a component of functional foods for the prevention of metabolic syndrome, due to its anti-inflammatory activity. We analyzed the effects of OA on inflammatory parameters and signaling proteins in LPS-stimulated THP-1 macrophages. Thus, THP-1 macrophages were incubated with LPS for 48 h after pretreatment with OA at different concentrations. Pretreatment with OA was significantly effective in attenuating IL-6 and TNF-α overproduction induced by LPS in macrophages, and also improved the levels of AMPK-α. We also evaluated the effects of human triglyceride-rich lipoproteins (TRLs) derived from individuals consuming an OA-enriched functional olive oil. For this purpose, TRLs were isolated from healthy adolescents before, 2 and 5 h postprandially after the intake of a meal containing the functional olive oil or common olive oil, and were incubated with THP-1 macrophages. THP-1 macrophages incubated with TRLs isolated at 2 h after the consumption of the OA-enriched olive oil showed significant lower levels of IL-6 compared to the TRLs derived from olive oil. Our results suggest that OA might have potential to be used as a lipid-based formulation in functional olive oils to prevent inflammatory processes underlying metabolic syndrome in adolescents.https://www.mdpi.com/2072-6643/13/10/3471oleanolic acidolive oilfunctional foodspostprandial trialhuman triglyceride-rich lipoproteinsmetabolic syndrome |
| spellingShingle | Ángel Fernández-Aparicio Javier S. Perona José M. Castellano María Correa-Rodríguez Jacqueline Schmidt-RioValle Emilio González-Jiménez Oleanolic Acid-Enriched Olive Oil Alleviates the Interleukin-6 Overproduction Induced by Postprandial Triglyceride-Rich Lipoproteins in THP-1 Macrophages Nutrients oleanolic acid olive oil functional foods postprandial trial human triglyceride-rich lipoproteins metabolic syndrome |
| title | Oleanolic Acid-Enriched Olive Oil Alleviates the Interleukin-6 Overproduction Induced by Postprandial Triglyceride-Rich Lipoproteins in THP-1 Macrophages |
| title_full | Oleanolic Acid-Enriched Olive Oil Alleviates the Interleukin-6 Overproduction Induced by Postprandial Triglyceride-Rich Lipoproteins in THP-1 Macrophages |
| title_fullStr | Oleanolic Acid-Enriched Olive Oil Alleviates the Interleukin-6 Overproduction Induced by Postprandial Triglyceride-Rich Lipoproteins in THP-1 Macrophages |
| title_full_unstemmed | Oleanolic Acid-Enriched Olive Oil Alleviates the Interleukin-6 Overproduction Induced by Postprandial Triglyceride-Rich Lipoproteins in THP-1 Macrophages |
| title_short | Oleanolic Acid-Enriched Olive Oil Alleviates the Interleukin-6 Overproduction Induced by Postprandial Triglyceride-Rich Lipoproteins in THP-1 Macrophages |
| title_sort | oleanolic acid enriched olive oil alleviates the interleukin 6 overproduction induced by postprandial triglyceride rich lipoproteins in thp 1 macrophages |
| topic | oleanolic acid olive oil functional foods postprandial trial human triglyceride-rich lipoproteins metabolic syndrome |
| url | https://www.mdpi.com/2072-6643/13/10/3471 |
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