Rhodnius (Stål, 1859) (Hemiptera, Triatominae) genus in Bolivian Amazonia: a risk for human populations?
Abstract Background Chagas disease, one of the most important neglected tropical diseases in the countries of Latin America, is considered to be a particularly important public health concern in the Amazon region due to increases in the number of outbreaks of acute Chagas disease and increased local...
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BMC
2022-08-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/s13071-022-05423-3 |
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| author | Stéphanie Depickère Anita G. Villacís Soledad Santillán-Guayasamín Jorgia Esperanza Callapa Rafael Simone Frédérique Brenière Susana Revollo Zepita |
| author_facet | Stéphanie Depickère Anita G. Villacís Soledad Santillán-Guayasamín Jorgia Esperanza Callapa Rafael Simone Frédérique Brenière Susana Revollo Zepita |
| author_sort | Stéphanie Depickère |
| collection | DOAJ |
| description | Abstract Background Chagas disease, one of the most important neglected tropical diseases in the countries of Latin America, is considered to be a particularly important public health concern in the Amazon region due to increases in the number of outbreaks of acute Chagas disease and increased local transmission in the last 20 years. However, relative to other countries, in Bolivia there is little information available on its transmission in the Amazon region. The aim of this study was to investigate the infestation of palm trees, the main habitat of Triatominae in the region, in several localities, to evaluate the danger they represent to inhabitants. Methods Triatominae were collected using live bait traps left overnight in six localities in Pando and Beni Departments, Bolivia. DNA extraction and sequencing were used to establish the Triatominae species (Cytb, 16S and 28S-D2 gene fragments), and the blood meal sources (Cytb fragment). Trypanosoma sp. infection was analyzed by sequencing gene fragments (GPX, GPI, HMCOAR, LAP, PDH and COII) or by mini-exon multiplex PCR. Results A total of 325 Rhodnius were captured (97.3% of nymphs) from the 1200 traps placed in 238 palm trees and 32 burrows/ground holes. Sequence analyses on DNA extracted from 114 insects and phylogeny analysis identified two triatomine species: Rhodnius stali (17%) and Rhodnius montenegrensis (equated to Rhodnius robustus II, 83%). These were found in palm trees of the genera Attalea (69%), Astrocaryum (13%), Copernicia (12%), Euterpe (2%) and Acrocomia (1%). The infection rate was around 30% (165 analyzed insects), with 90% of analyzed insects infected by Trypanosoma cruzi (only the TcI discrete typing unit was detected), 3% infected by Trypanosoma rangeli (first time found in Bolivian Triatominae) and 7% infected by mixed T. cruzi (TcI)-T. rangeli. Rhodnius specimens fed on Didelphidae, rodents, gecko and humans. Conclusions The results of this study highlight the epidemiological importance of Rhodnius in the Bolivian Amazon region. The huge geographical distribution of Rhodnius and their proximity to the human dwellings, high infection rate and frequent meals on the human population highlight a risk of transmission of Chagas disease in the region. Graphical Abstract |
| first_indexed | 2024-04-12T23:28:26Z |
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| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-04-12T23:28:26Z |
| publishDate | 2022-08-01 |
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| spelling | doaj.art-a7546d0eca0c4ae68386bbc9e3dc794f2022-12-22T03:12:21ZengBMCParasites & Vectors1756-33052022-08-0115112210.1186/s13071-022-05423-3Rhodnius (Stål, 1859) (Hemiptera, Triatominae) genus in Bolivian Amazonia: a risk for human populations?Stéphanie Depickère0Anita G. Villacís1Soledad Santillán-Guayasamín2Jorgia Esperanza Callapa Rafael3Simone Frédérique Brenière4Susana Revollo Zepita5Instituto de Investigaciones Físicas, Universidad Mayor de San AndrésCentro de Investigación para la Salud en América Latina (CISeAL), Escuela de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Pontificia Universidad Católica del EcuadorCentro de Investigación para la Salud en América Latina (CISeAL), Escuela de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Pontificia Universidad Católica del EcuadorLaboratorio de Genética Molecular (SELADIS), Facultad Ciencias Farmacéuticas y Bioquímicas, Universidad Mayor de San AndrésInstitut de Recherche pour le Développement (IRD), UMR INTERTRYP IRD-CIRAD, University of MontpellierLaboratorio de Genética Molecular (SELADIS), Facultad Ciencias Farmacéuticas y Bioquímicas, Universidad Mayor de San AndrésAbstract Background Chagas disease, one of the most important neglected tropical diseases in the countries of Latin America, is considered to be a particularly important public health concern in the Amazon region due to increases in the number of outbreaks of acute Chagas disease and increased local transmission in the last 20 years. However, relative to other countries, in Bolivia there is little information available on its transmission in the Amazon region. The aim of this study was to investigate the infestation of palm trees, the main habitat of Triatominae in the region, in several localities, to evaluate the danger they represent to inhabitants. Methods Triatominae were collected using live bait traps left overnight in six localities in Pando and Beni Departments, Bolivia. DNA extraction and sequencing were used to establish the Triatominae species (Cytb, 16S and 28S-D2 gene fragments), and the blood meal sources (Cytb fragment). Trypanosoma sp. infection was analyzed by sequencing gene fragments (GPX, GPI, HMCOAR, LAP, PDH and COII) or by mini-exon multiplex PCR. Results A total of 325 Rhodnius were captured (97.3% of nymphs) from the 1200 traps placed in 238 palm trees and 32 burrows/ground holes. Sequence analyses on DNA extracted from 114 insects and phylogeny analysis identified two triatomine species: Rhodnius stali (17%) and Rhodnius montenegrensis (equated to Rhodnius robustus II, 83%). These were found in palm trees of the genera Attalea (69%), Astrocaryum (13%), Copernicia (12%), Euterpe (2%) and Acrocomia (1%). The infection rate was around 30% (165 analyzed insects), with 90% of analyzed insects infected by Trypanosoma cruzi (only the TcI discrete typing unit was detected), 3% infected by Trypanosoma rangeli (first time found in Bolivian Triatominae) and 7% infected by mixed T. cruzi (TcI)-T. rangeli. Rhodnius specimens fed on Didelphidae, rodents, gecko and humans. Conclusions The results of this study highlight the epidemiological importance of Rhodnius in the Bolivian Amazon region. The huge geographical distribution of Rhodnius and their proximity to the human dwellings, high infection rate and frequent meals on the human population highlight a risk of transmission of Chagas disease in the region. Graphical Abstracthttps://doi.org/10.1186/s13071-022-05423-3ChagasTriatominaeRhodnius staliRhodnius montenegrensisTrypanosoma cruziDTUs |
| spellingShingle | Stéphanie Depickère Anita G. Villacís Soledad Santillán-Guayasamín Jorgia Esperanza Callapa Rafael Simone Frédérique Brenière Susana Revollo Zepita Rhodnius (Stål, 1859) (Hemiptera, Triatominae) genus in Bolivian Amazonia: a risk for human populations? Parasites & Vectors Chagas Triatominae Rhodnius stali Rhodnius montenegrensis Trypanosoma cruzi DTUs |
| title | Rhodnius (Stål, 1859) (Hemiptera, Triatominae) genus in Bolivian Amazonia: a risk for human populations? |
| title_full | Rhodnius (Stål, 1859) (Hemiptera, Triatominae) genus in Bolivian Amazonia: a risk for human populations? |
| title_fullStr | Rhodnius (Stål, 1859) (Hemiptera, Triatominae) genus in Bolivian Amazonia: a risk for human populations? |
| title_full_unstemmed | Rhodnius (Stål, 1859) (Hemiptera, Triatominae) genus in Bolivian Amazonia: a risk for human populations? |
| title_short | Rhodnius (Stål, 1859) (Hemiptera, Triatominae) genus in Bolivian Amazonia: a risk for human populations? |
| title_sort | rhodnius stal 1859 hemiptera triatominae genus in bolivian amazonia a risk for human populations |
| topic | Chagas Triatominae Rhodnius stali Rhodnius montenegrensis Trypanosoma cruzi DTUs |
| url | https://doi.org/10.1186/s13071-022-05423-3 |
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