Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules
Midhun B Thomas,1,* Krishna Radhakrishnan,1,* Divya P Gnanadhas,2,* Dipshikha Chakravortty,2 Ashok M Raichur1,31Department of Materials Engineering, 2Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India; 3Department of Applied Chemistry, University of Johannesbu...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2013-01-01
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| Series: | International Journal of Nanomedicine |
| Online Access: | http://www.dovepress.com/intracellular-delivery-of-doxorubicin-encapsulated-in-novel-ph-respons-a11940 |
| _version_ | 1818742244674371584 |
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| author | Thomas MB Radhakrishnan K Gnanadhas DP Chakravortty D Raichur AM |
| author_facet | Thomas MB Radhakrishnan K Gnanadhas DP Chakravortty D Raichur AM |
| author_sort | Thomas MB |
| collection | DOAJ |
| description | Midhun B Thomas,1,* Krishna Radhakrishnan,1,* Divya P Gnanadhas,2,* Dipshikha Chakravortty,2 Ashok M Raichur1,31Department of Materials Engineering, 2Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India; 3Department of Applied Chemistry, University of Johannesburg, Doornfontein, South Africa*These authors contributed equally to this workAbstract: A novel polyelectrolyte nanocapsule system composed of biopolymers, chitosan and heparin has been fabricated by the layer-by-layer technique on silica nanoparticles followed by dissolution of the silica core. The nanocapsules were of the size range 200 ± 20 nm and loaded with the positively charged anticancer drug doxorubicin with an efficiency of 89%. The loading of the drug into the capsule happens by virtue of the pH-responsive property of the capsule wall, which is determined by the pKa of the polyelectrolytes. As the pH is varied, about 64% of the drug is released in acidic pH while 77% is released in neutral pH. The biocompatibility, efficiency of drug loading, and enhanced bioavailability of the capsule system was confirmed by MTT assay and in vivo biodistribution studies.Keywords: drug delivery, layer-by-layer, electrostatic interaction, biocompatible |
| first_indexed | 2024-12-18T02:09:26Z |
| format | Article |
| id | doaj.art-a758bc4b0d0c4d7cbb456a73f5bcdbae |
| institution | Directory Open Access Journal |
| issn | 1176-9114 1178-2013 |
| language | English |
| last_indexed | 2024-12-18T02:09:26Z |
| publishDate | 2013-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-a758bc4b0d0c4d7cbb456a73f5bcdbae2022-12-21T21:24:31ZengDove Medical PressInternational Journal of Nanomedicine1176-91141178-20132013-01-012013default267273Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsulesThomas MBRadhakrishnan KGnanadhas DPChakravortty DRaichur AMMidhun B Thomas,1,* Krishna Radhakrishnan,1,* Divya P Gnanadhas,2,* Dipshikha Chakravortty,2 Ashok M Raichur1,31Department of Materials Engineering, 2Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India; 3Department of Applied Chemistry, University of Johannesburg, Doornfontein, South Africa*These authors contributed equally to this workAbstract: A novel polyelectrolyte nanocapsule system composed of biopolymers, chitosan and heparin has been fabricated by the layer-by-layer technique on silica nanoparticles followed by dissolution of the silica core. The nanocapsules were of the size range 200 ± 20 nm and loaded with the positively charged anticancer drug doxorubicin with an efficiency of 89%. The loading of the drug into the capsule happens by virtue of the pH-responsive property of the capsule wall, which is determined by the pKa of the polyelectrolytes. As the pH is varied, about 64% of the drug is released in acidic pH while 77% is released in neutral pH. The biocompatibility, efficiency of drug loading, and enhanced bioavailability of the capsule system was confirmed by MTT assay and in vivo biodistribution studies.Keywords: drug delivery, layer-by-layer, electrostatic interaction, biocompatiblehttp://www.dovepress.com/intracellular-delivery-of-doxorubicin-encapsulated-in-novel-ph-respons-a11940 |
| spellingShingle | Thomas MB Radhakrishnan K Gnanadhas DP Chakravortty D Raichur AM Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules International Journal of Nanomedicine |
| title | Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules |
| title_full | Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules |
| title_fullStr | Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules |
| title_full_unstemmed | Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules |
| title_short | Intracellular delivery of doxorubicin encapsulated in novel pH-responsive chitosan/heparin nanocapsules |
| title_sort | intracellular delivery of doxorubicin encapsulated in novel ph responsive chitosan heparin nanocapsules |
| url | http://www.dovepress.com/intracellular-delivery-of-doxorubicin-encapsulated-in-novel-ph-respons-a11940 |
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