Liquid Biopsy in Colorectal Carcinoma: Clinical Applications and Challenges
Colorectal carcinoma (CRC) is characterized by wide intratumor heterogeneity with general genomic instability and there is a need for improved diagnostic, prognostic, and therapeutic tools. The liquid biopsy provides a noninvasive route of sample collection for analysis of circulating tumor cells (C...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/12/6/1376 |
| _version_ | 1797566900060815360 |
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| author | Drahomír Kolenčík Stephanie N. Shishido Pavel Pitule Jeremy Mason James Hicks Peter Kuhn |
| author_facet | Drahomír Kolenčík Stephanie N. Shishido Pavel Pitule Jeremy Mason James Hicks Peter Kuhn |
| author_sort | Drahomír Kolenčík |
| collection | DOAJ |
| description | Colorectal carcinoma (CRC) is characterized by wide intratumor heterogeneity with general genomic instability and there is a need for improved diagnostic, prognostic, and therapeutic tools. The liquid biopsy provides a noninvasive route of sample collection for analysis of circulating tumor cells (CTCs) and genomic material, including cell-free DNA (cfDNA), as a complementary biopsy to the solid tumor tissue. The solid biopsy is critical for molecular characterization and diagnosis at the time of collection. The liquid biopsy has the advantage of longitudinal molecular characterization of the disease, which is crucial for precision medicine and patient-oriented treatment. In this review, we provide an overview of CRC and the different methodologies for the detection of CTCs and cfDNA, followed by a discussion on the potential clinical utility of the liquid biopsy in CRC patient care, and lastly, current challenges in the field. |
| first_indexed | 2024-03-10T19:33:56Z |
| format | Article |
| id | doaj.art-a75b522935bb439583286d1189cdf539 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T19:33:56Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-a75b522935bb439583286d1189cdf5392023-11-20T01:54:32ZengMDPI AGCancers2072-66942020-05-01126137610.3390/cancers12061376Liquid Biopsy in Colorectal Carcinoma: Clinical Applications and ChallengesDrahomír Kolenčík0Stephanie N. Shishido1Pavel Pitule2Jeremy Mason3James Hicks4Peter Kuhn5Biomedical Centre, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech RepublicConvergent Science Institute in Cancer, Michelson Center for Convergent Bioscience, Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA 90089, USABiomedical Centre, Faculty of Medicine in Pilsen, Charles University, 32300 Pilsen, Czech RepublicConvergent Science Institute in Cancer, Michelson Center for Convergent Bioscience, Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA 90089, USAConvergent Science Institute in Cancer, Michelson Center for Convergent Bioscience, Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA 90089, USAConvergent Science Institute in Cancer, Michelson Center for Convergent Bioscience, Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA 90089, USAColorectal carcinoma (CRC) is characterized by wide intratumor heterogeneity with general genomic instability and there is a need for improved diagnostic, prognostic, and therapeutic tools. The liquid biopsy provides a noninvasive route of sample collection for analysis of circulating tumor cells (CTCs) and genomic material, including cell-free DNA (cfDNA), as a complementary biopsy to the solid tumor tissue. The solid biopsy is critical for molecular characterization and diagnosis at the time of collection. The liquid biopsy has the advantage of longitudinal molecular characterization of the disease, which is crucial for precision medicine and patient-oriented treatment. In this review, we provide an overview of CRC and the different methodologies for the detection of CTCs and cfDNA, followed by a discussion on the potential clinical utility of the liquid biopsy in CRC patient care, and lastly, current challenges in the field.https://www.mdpi.com/2072-6694/12/6/1376colorectal carcinomaCRCliquid biopsycirculating tumor cellCTCcirculating tumor DNA |
| spellingShingle | Drahomír Kolenčík Stephanie N. Shishido Pavel Pitule Jeremy Mason James Hicks Peter Kuhn Liquid Biopsy in Colorectal Carcinoma: Clinical Applications and Challenges Cancers colorectal carcinoma CRC liquid biopsy circulating tumor cell CTC circulating tumor DNA |
| title | Liquid Biopsy in Colorectal Carcinoma: Clinical Applications and Challenges |
| title_full | Liquid Biopsy in Colorectal Carcinoma: Clinical Applications and Challenges |
| title_fullStr | Liquid Biopsy in Colorectal Carcinoma: Clinical Applications and Challenges |
| title_full_unstemmed | Liquid Biopsy in Colorectal Carcinoma: Clinical Applications and Challenges |
| title_short | Liquid Biopsy in Colorectal Carcinoma: Clinical Applications and Challenges |
| title_sort | liquid biopsy in colorectal carcinoma clinical applications and challenges |
| topic | colorectal carcinoma CRC liquid biopsy circulating tumor cell CTC circulating tumor DNA |
| url | https://www.mdpi.com/2072-6694/12/6/1376 |
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