Comparison of the Effect of the Amino Acids on Spontaneous Formation and Transformation of Calcium Phosphates
Understanding the effect that specific amino acids (AA) exert on calcium phosphate (CaPs) formation is proposed as a way of providing deeper insight into CaPs’ biomineralization and enabling the design of tailored-made additives for the synthesis of functional materials. Despite a number of investig...
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MDPI AG
2021-07-01
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| Online Access: | https://www.mdpi.com/2073-4352/11/7/792 |
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| author | Ina Erceg Nadica Maltar-Strmečki Darija Domazet Jurašin Vida Strasser Marija Ćurlin Daniel Mark Lyons Borna Radatović Nives Matijaković Mlinarić Damir Kralj Maja Dutour Sikirić |
| author_facet | Ina Erceg Nadica Maltar-Strmečki Darija Domazet Jurašin Vida Strasser Marija Ćurlin Daniel Mark Lyons Borna Radatović Nives Matijaković Mlinarić Damir Kralj Maja Dutour Sikirić |
| author_sort | Ina Erceg |
| collection | DOAJ |
| description | Understanding the effect that specific amino acids (AA) exert on calcium phosphate (CaPs) formation is proposed as a way of providing deeper insight into CaPs’ biomineralization and enabling the design of tailored-made additives for the synthesis of functional materials. Despite a number of investigations, the role of specific AA is still unclear, mostly because markedly different experimental conditions have been employed in different studies. The aim of this paper was to compare the influence of different classes of amino acids, charged (aspartic acid, Asp and lysine, Lys), polar (asparagine, Asn and serine, Ser) and non-polar (phenylalanine, Phe) on CaPs formation and transformation in conditions similar to physiological conditions. The precipitation process was followed potentiometrically, while Fourier transform infrared spectroscopy, powder X-ray diffraction, electron paramagnetic spectroscopy (EPR), scanning and transmission electron microscopy were used for the characterization of precipitates. Except for Phe, all investigated AAs inhibited amorphous calcium phosphate (ACP) transformation, with Ser being the most efficient inhibitor. In all systems, ACP transformed in calcium-deficient hydroxyapatite (CaDHA). However, the size of crystalline domains was affected, as well as CaDHA morphology. In EPR spectra, the contribution of different radical species with different proportions in diverse surroundings, depending on the type of AA present, was observed. The obtained results are of interest for the preparation of functionalized CaPs’, as well as for the understanding of their formation in vivo. |
| first_indexed | 2024-03-10T09:42:19Z |
| format | Article |
| id | doaj.art-a75c3553d29c40ea99b7599b07510392 |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-03-10T09:42:19Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-a75c3553d29c40ea99b7599b075103922023-11-22T03:33:09ZengMDPI AGCrystals2073-43522021-07-0111779210.3390/cryst11070792Comparison of the Effect of the Amino Acids on Spontaneous Formation and Transformation of Calcium PhosphatesIna Erceg0Nadica Maltar-Strmečki1Darija Domazet Jurašin2Vida Strasser3Marija Ćurlin4Daniel Mark Lyons5Borna Radatović6Nives Matijaković Mlinarić7Damir Kralj8Maja Dutour Sikirić9Laboratory for Biocolloids and Surface Chemistry, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, CroatiaLaboratory for Magnetic Resonances, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, CroatiaLaboratory for Biocolloids and Surface Chemistry, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, CroatiaLaboratory for Biocolloids and Surface Chemistry, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, CroatiaDepartment of Histology and Embryology, School of Medicine, University of Zagreb, Šalata 3, 10000 Zagreb, CroatiaCenter for Marine Research, Ruđer Bošković Institute, Giordano Paliaga 5, 52210 Rovinj, CroatiaInstitute for Physics, Bijenička cesta 46, 10000 Zagreb, CroatiaLaboratory for Precipitation Processes, Division of Materials Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, CroatiaLaboratory for Precipitation Processes, Division of Materials Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, CroatiaLaboratory for Biocolloids and Surface Chemistry, Division of Physical Chemistry, Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, CroatiaUnderstanding the effect that specific amino acids (AA) exert on calcium phosphate (CaPs) formation is proposed as a way of providing deeper insight into CaPs’ biomineralization and enabling the design of tailored-made additives for the synthesis of functional materials. Despite a number of investigations, the role of specific AA is still unclear, mostly because markedly different experimental conditions have been employed in different studies. The aim of this paper was to compare the influence of different classes of amino acids, charged (aspartic acid, Asp and lysine, Lys), polar (asparagine, Asn and serine, Ser) and non-polar (phenylalanine, Phe) on CaPs formation and transformation in conditions similar to physiological conditions. The precipitation process was followed potentiometrically, while Fourier transform infrared spectroscopy, powder X-ray diffraction, electron paramagnetic spectroscopy (EPR), scanning and transmission electron microscopy were used for the characterization of precipitates. Except for Phe, all investigated AAs inhibited amorphous calcium phosphate (ACP) transformation, with Ser being the most efficient inhibitor. In all systems, ACP transformed in calcium-deficient hydroxyapatite (CaDHA). However, the size of crystalline domains was affected, as well as CaDHA morphology. In EPR spectra, the contribution of different radical species with different proportions in diverse surroundings, depending on the type of AA present, was observed. The obtained results are of interest for the preparation of functionalized CaPs’, as well as for the understanding of their formation in vivo.https://www.mdpi.com/2073-4352/11/7/792amino acidscalcium phosphatesspontaneous precipitationtransformation |
| spellingShingle | Ina Erceg Nadica Maltar-Strmečki Darija Domazet Jurašin Vida Strasser Marija Ćurlin Daniel Mark Lyons Borna Radatović Nives Matijaković Mlinarić Damir Kralj Maja Dutour Sikirić Comparison of the Effect of the Amino Acids on Spontaneous Formation and Transformation of Calcium Phosphates Crystals amino acids calcium phosphates spontaneous precipitation transformation |
| title | Comparison of the Effect of the Amino Acids on Spontaneous Formation and Transformation of Calcium Phosphates |
| title_full | Comparison of the Effect of the Amino Acids on Spontaneous Formation and Transformation of Calcium Phosphates |
| title_fullStr | Comparison of the Effect of the Amino Acids on Spontaneous Formation and Transformation of Calcium Phosphates |
| title_full_unstemmed | Comparison of the Effect of the Amino Acids on Spontaneous Formation and Transformation of Calcium Phosphates |
| title_short | Comparison of the Effect of the Amino Acids on Spontaneous Formation and Transformation of Calcium Phosphates |
| title_sort | comparison of the effect of the amino acids on spontaneous formation and transformation of calcium phosphates |
| topic | amino acids calcium phosphates spontaneous precipitation transformation |
| url | https://www.mdpi.com/2073-4352/11/7/792 |
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