First record of entomopathogenic nematodes from Yucatán State, México and their infectivity capacity against Aedes aegypti
Background Biological control using entomopathogenic nematodes (EPN) has demonstrated good potential to contribute to the integral control of mosquito larvae, which as adults are vectors of diseases such as Dengue fever, Zika and Chikungunya. However, until now there are no records of the presence o...
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PeerJ Inc.
2021-07-01
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| author | Mariana B. Ávila-López José Q. García-Maldonado Héctor Estrada-Medina David I. Hernández-Mena Daniel Cerqueda-García Víctor M. Vidal-Martínez |
| author_facet | Mariana B. Ávila-López José Q. García-Maldonado Héctor Estrada-Medina David I. Hernández-Mena Daniel Cerqueda-García Víctor M. Vidal-Martínez |
| author_sort | Mariana B. Ávila-López |
| collection | DOAJ |
| description | Background Biological control using entomopathogenic nematodes (EPN) has demonstrated good potential to contribute to the integral control of mosquito larvae, which as adults are vectors of diseases such as Dengue fever, Zika and Chikungunya. However, until now there are no records of the presence of EPN or their killing capacity in Yucatán state, southern México. The objectives of the current study were: (1) to report the entomopathogenic nematodes present in Yucatán soils and (2) to determine the killing capacity of the most frequent and abundant EPN against Aedes aegypti mosquito larvae and the microbial community developed by Ae. Aegypti exposed to this EPN. Methods The nematodes were collected by the insect trap technique using the great wax moth Galleria mellonella. Internal transcribed spacer (ITS), 28S gene of ribosomal DNA and phylogenetic analyses were performed to identify the EPN. For the bioassay, four concentrations of the most frequent and abundant EPN were tested: 1,260:1 infective juveniles (IJs) per mosquito larvae, 2,520 IJs:1, 3,780 IJs:1 and 5,040 IJs:1. High-throughput sequencing of the 16S rRNA gene was used to identify bacterial amplicon sequences in the mosquito larvae infected with EPN. Results Six isolates of Heterorhabditis were recovered from 144 soil samples. Heterorhabditis indica (four isolates) was the most frequent and abundant EPN, followed by Heterorhabditis n. sp. (two isolates). Both nematodes are reported for the first time for Yucatán state, Mexico. The concentration of 2,520 IJs:1 produced 80% of mosquito larvae mortality in 48 h. Representative members of Photorhabdus genus were numerically dominant (74%) in mosquito larvae infected by H. indica. It is most likely that these bacteria produce secondary toxic metabolites that enhance the mortality of these mosquito larvae. |
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| language | English |
| last_indexed | 2024-03-09T06:36:26Z |
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| spelling | doaj.art-a75f1bc1219644fb85696cf928cf2c9b2023-12-03T10:57:38ZengPeerJ Inc.PeerJ2167-83592021-07-019e1163310.7717/peerj.11633First record of entomopathogenic nematodes from Yucatán State, México and their infectivity capacity against Aedes aegyptiMariana B. Ávila-López0José Q. García-Maldonado1Héctor Estrada-Medina2David I. Hernández-Mena3Daniel Cerqueda-García4Víctor M. Vidal-Martínez5Aquatic Pathology Laboratory. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Unidad Mérida, Carretera Antigua a Progreso,, Mérida, Yucatán, MéxicoAquatic Pathology Laboratory. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Unidad Mérida, Carretera Antigua a Progreso,, Mérida, Yucatán, MéxicoFacultad de Medicina Veterinaria y Zootecnia, Departamento de Manejo y Conservación de Recursos Naturales Tropicales, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Mérida, Yucatán, MéxicoAquatic Pathology Laboratory. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Unidad Mérida, Carretera Antigua a Progreso,, Mérida, Yucatán, MéxicoAquatic Pathology Laboratory. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Unidad Mérida, Carretera Antigua a Progreso,, Mérida, Yucatán, MéxicoAquatic Pathology Laboratory. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Unidad Mérida, Carretera Antigua a Progreso,, Mérida, Yucatán, MéxicoBackground Biological control using entomopathogenic nematodes (EPN) has demonstrated good potential to contribute to the integral control of mosquito larvae, which as adults are vectors of diseases such as Dengue fever, Zika and Chikungunya. However, until now there are no records of the presence of EPN or their killing capacity in Yucatán state, southern México. The objectives of the current study were: (1) to report the entomopathogenic nematodes present in Yucatán soils and (2) to determine the killing capacity of the most frequent and abundant EPN against Aedes aegypti mosquito larvae and the microbial community developed by Ae. Aegypti exposed to this EPN. Methods The nematodes were collected by the insect trap technique using the great wax moth Galleria mellonella. Internal transcribed spacer (ITS), 28S gene of ribosomal DNA and phylogenetic analyses were performed to identify the EPN. For the bioassay, four concentrations of the most frequent and abundant EPN were tested: 1,260:1 infective juveniles (IJs) per mosquito larvae, 2,520 IJs:1, 3,780 IJs:1 and 5,040 IJs:1. High-throughput sequencing of the 16S rRNA gene was used to identify bacterial amplicon sequences in the mosquito larvae infected with EPN. Results Six isolates of Heterorhabditis were recovered from 144 soil samples. Heterorhabditis indica (four isolates) was the most frequent and abundant EPN, followed by Heterorhabditis n. sp. (two isolates). Both nematodes are reported for the first time for Yucatán state, Mexico. The concentration of 2,520 IJs:1 produced 80% of mosquito larvae mortality in 48 h. Representative members of Photorhabdus genus were numerically dominant (74%) in mosquito larvae infected by H. indica. It is most likely that these bacteria produce secondary toxic metabolites that enhance the mortality of these mosquito larvae.https://peerj.com/articles/11633.pdfAedes aegyptiBiological controlGalleria mellonellaHeterorhabditis indicaPhotorhabdus16S rRNA |
| spellingShingle | Mariana B. Ávila-López José Q. García-Maldonado Héctor Estrada-Medina David I. Hernández-Mena Daniel Cerqueda-García Víctor M. Vidal-Martínez First record of entomopathogenic nematodes from Yucatán State, México and their infectivity capacity against Aedes aegypti PeerJ Aedes aegypti Biological control Galleria mellonella Heterorhabditis indica Photorhabdus 16S rRNA |
| title | First record of entomopathogenic nematodes from Yucatán State, México and their infectivity capacity against Aedes aegypti |
| title_full | First record of entomopathogenic nematodes from Yucatán State, México and their infectivity capacity against Aedes aegypti |
| title_fullStr | First record of entomopathogenic nematodes from Yucatán State, México and their infectivity capacity against Aedes aegypti |
| title_full_unstemmed | First record of entomopathogenic nematodes from Yucatán State, México and their infectivity capacity against Aedes aegypti |
| title_short | First record of entomopathogenic nematodes from Yucatán State, México and their infectivity capacity against Aedes aegypti |
| title_sort | first record of entomopathogenic nematodes from yucatan state mexico and their infectivity capacity against aedes aegypti |
| topic | Aedes aegypti Biological control Galleria mellonella Heterorhabditis indica Photorhabdus 16S rRNA |
| url | https://peerj.com/articles/11633.pdf |
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