Aerobic vaginitis is associated with carbonic anhydrase IX in cervical intraepithelial neoplasia
Abstract The aim of this study was to analyze the association between vaginal microbiota, carbonic anhydrase IX (CAIX) and histological findings of cervical intraepithelial neoplasia (CIN). The study included 132 females, among them 66 were diagnosed with high-grade intraepithelial lesion (CIN2, CIN...
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| Format: | Article |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-57427-x |
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| author | Švitrigailė Grincevičienė Daiva Vaitkienė Daiva Kanopienė Rasa Vansevičiūtė Petkevičienė Artūras Sukovas Joana Celiešiūtė Ernesta Ivanauskaitė Didžiokienė Arvydas Čižauskas Aida Laurinavičienė Dovilė Stravinskienė Jonas Grincevičius Daumantas Matulis Jurgita Matulienė |
| author_facet | Švitrigailė Grincevičienė Daiva Vaitkienė Daiva Kanopienė Rasa Vansevičiūtė Petkevičienė Artūras Sukovas Joana Celiešiūtė Ernesta Ivanauskaitė Didžiokienė Arvydas Čižauskas Aida Laurinavičienė Dovilė Stravinskienė Jonas Grincevičius Daumantas Matulis Jurgita Matulienė |
| author_sort | Švitrigailė Grincevičienė |
| collection | DOAJ |
| description | Abstract The aim of this study was to analyze the association between vaginal microbiota, carbonic anhydrase IX (CAIX) and histological findings of cervical intraepithelial neoplasia (CIN). The study included 132 females, among them 66 were diagnosed with high-grade intraepithelial lesion (CIN2, CIN3, and cancer), 14 with low-grade disease, and 52 assigned to the control group. An interview focused on the behavior risk factors, together with vaginal fluid pH measurement, wet mount microscopy, detection of Chlamydia trachomatis, and Trichomonas vaginalis were performed. After colposcopy, high-grade abnormalities were detected via direct biopsies and treated with conization procedure. Conuses were immuno-stained with CAIX antibody. The histological findings were CIN1 (n = 14), and CIN2+ (included CIN2 (n = 10), CIN3 (n = 49), and cancer (n = 7; squamous cell carcinomas)). Prevalence of bacterial vaginosis (BV) was similar between the groups. Moderate or severe aerobic vaginitis (msAV) was diagnosed more often among CIN2+ (53.0%) than CIN1 (21.4%). Moderate or strong immunostaining of CAIX (msCAIX) was not detected among CIN1 cases. Thus, msAV was prevalent in CAIX non-stained group (p = 0.049) among CIN2 patients. Co-location of msAV and msCAIX was found in CIN3. Regression model revealed that msAV associated with high-grade cervical intraepithelial neoplasia independently from smoking and the number of partners. |
| first_indexed | 2024-04-24T07:16:32Z |
| format | Article |
| id | doaj.art-a75f7ae061c44f17b4dccb008ca22500 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T07:16:32Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a75f7ae061c44f17b4dccb008ca225002024-04-21T11:16:41ZengNature PortfolioScientific Reports2045-23222024-04-0114111010.1038/s41598-024-57427-xAerobic vaginitis is associated with carbonic anhydrase IX in cervical intraepithelial neoplasiaŠvitrigailė Grincevičienė0Daiva Vaitkienė1Daiva Kanopienė2Rasa Vansevičiūtė Petkevičienė3Artūras Sukovas4Joana Celiešiūtė5Ernesta Ivanauskaitė Didžiokienė6Arvydas Čižauskas7Aida Laurinavičienė8Dovilė Stravinskienė9Jonas Grincevičius10Daumantas Matulis11Jurgita Matulienė12Department of Biothermodynamics and Drug Design, Institute of Biotechnology, Life Sciences Center, Vilnius UniversityDepartment of Obstetrics and Gynecology, Medical Academy, Lithuanian University of Health SciencesConsultative Polyclinic Department, National Cancer InstituteConsultative Polyclinic Department, National Cancer InstituteDepartment of Obstetrics and Gynecology, Medical Academy, Lithuanian University of Health SciencesDepartment of Obstetrics and Gynecology, Medical Academy, Lithuanian University of Health SciencesNational Center of Pathology, Affiliate of Vilnius University Hospital Santaros KlinikosDepartment of Pathological Anatomy, Medical Academy, Lithuanian University of Health SciencesNational Center of Pathology, Affiliate of Vilnius University Hospital Santaros KlinikosDepartment of Immunology and Cell Biology, Institute of Biotechnology, Life Sciences Center, Vilnius UniversityFaculty of Medicine, Pharmacy and Pharmacology Center, Institute of Biomedical Science, Vilnius UniversityDepartment of Biothermodynamics and Drug Design, Institute of Biotechnology, Life Sciences Center, Vilnius UniversityDepartment of Biothermodynamics and Drug Design, Institute of Biotechnology, Life Sciences Center, Vilnius UniversityAbstract The aim of this study was to analyze the association between vaginal microbiota, carbonic anhydrase IX (CAIX) and histological findings of cervical intraepithelial neoplasia (CIN). The study included 132 females, among them 66 were diagnosed with high-grade intraepithelial lesion (CIN2, CIN3, and cancer), 14 with low-grade disease, and 52 assigned to the control group. An interview focused on the behavior risk factors, together with vaginal fluid pH measurement, wet mount microscopy, detection of Chlamydia trachomatis, and Trichomonas vaginalis were performed. After colposcopy, high-grade abnormalities were detected via direct biopsies and treated with conization procedure. Conuses were immuno-stained with CAIX antibody. The histological findings were CIN1 (n = 14), and CIN2+ (included CIN2 (n = 10), CIN3 (n = 49), and cancer (n = 7; squamous cell carcinomas)). Prevalence of bacterial vaginosis (BV) was similar between the groups. Moderate or severe aerobic vaginitis (msAV) was diagnosed more often among CIN2+ (53.0%) than CIN1 (21.4%). Moderate or strong immunostaining of CAIX (msCAIX) was not detected among CIN1 cases. Thus, msAV was prevalent in CAIX non-stained group (p = 0.049) among CIN2 patients. Co-location of msAV and msCAIX was found in CIN3. Regression model revealed that msAV associated with high-grade cervical intraepithelial neoplasia independently from smoking and the number of partners.https://doi.org/10.1038/s41598-024-57427-xCAIX enzymeCarbonic anhydrase 9Cervical intraepithelial neoplasiaUterine cervix dysplasiaAerobic vaginitisMicrobiota alteration |
| spellingShingle | Švitrigailė Grincevičienė Daiva Vaitkienė Daiva Kanopienė Rasa Vansevičiūtė Petkevičienė Artūras Sukovas Joana Celiešiūtė Ernesta Ivanauskaitė Didžiokienė Arvydas Čižauskas Aida Laurinavičienė Dovilė Stravinskienė Jonas Grincevičius Daumantas Matulis Jurgita Matulienė Aerobic vaginitis is associated with carbonic anhydrase IX in cervical intraepithelial neoplasia Scientific Reports CAIX enzyme Carbonic anhydrase 9 Cervical intraepithelial neoplasia Uterine cervix dysplasia Aerobic vaginitis Microbiota alteration |
| title | Aerobic vaginitis is associated with carbonic anhydrase IX in cervical intraepithelial neoplasia |
| title_full | Aerobic vaginitis is associated with carbonic anhydrase IX in cervical intraepithelial neoplasia |
| title_fullStr | Aerobic vaginitis is associated with carbonic anhydrase IX in cervical intraepithelial neoplasia |
| title_full_unstemmed | Aerobic vaginitis is associated with carbonic anhydrase IX in cervical intraepithelial neoplasia |
| title_short | Aerobic vaginitis is associated with carbonic anhydrase IX in cervical intraepithelial neoplasia |
| title_sort | aerobic vaginitis is associated with carbonic anhydrase ix in cervical intraepithelial neoplasia |
| topic | CAIX enzyme Carbonic anhydrase 9 Cervical intraepithelial neoplasia Uterine cervix dysplasia Aerobic vaginitis Microbiota alteration |
| url | https://doi.org/10.1038/s41598-024-57427-x |
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