RNAi-Mediated Transgenic Tospovirus Resistance Broken by Intraspecies Silencing Suppressor Protein Complementation
Extension of an inverted repeat transgene cassette, containing partial nucleoprotein (N) gene sequences from four different tomato-infecting Tospovirus spp. with a partial N gene sequence from the tomato strain of Tomato yellow ring virus (TYRV-t), renders transgenic Nicotiana benthamiana plants add...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2009-10-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-10-1250 |
| _version_ | 1818757356907921408 |
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| author | Afshin Hassani-Mehraban A. B. Brenkman N. J. F. van den Broek Rob Goldbach Richard Kormelink |
| author_facet | Afshin Hassani-Mehraban A. B. Brenkman N. J. F. van den Broek Rob Goldbach Richard Kormelink |
| author_sort | Afshin Hassani-Mehraban |
| collection | DOAJ |
| description | Extension of an inverted repeat transgene cassette, containing partial nucleoprotein (N) gene sequences from four different tomato-infecting Tospovirus spp. with a partial N gene sequence from the tomato strain of Tomato yellow ring virus (TYRV-t), renders transgenic Nicotiana benthamiana plants additionally resistant to this strain but not to the soybean strain of this Tospovirus sp. (TYRV-s), both strains exhibiting 14.4% nucleotide sequence divergence in their N genes. Surprisingly, coinoculation of the TYRV-t-resistant transgenic lines with both TYRV-t and TYRV-s resulted in rescue of the former. Mass-spectrometric analysis of the viral ribonucleocapsids accumulating in the transgenic plants showed the presence of the N proteins of both strains excluding hetero-encapsidation as rescue mechanism and indicating suppression of TYRV-t N gene transcript breakdown by RNA interference. Prior (Potato virus X [PVX]-vector-mediated) expression of the TYRV-s silencing suppressor (NSs) gene also allowed TYRV-t to break the resistance. This phenomenon was also observed when the homologous (TYRV-t) NSs gene was provided from a PVX replicon, demonstrating that TYRV can break RNA-mediated host resistance upon a priori expression of its NSs protein. Remarkably, mixed inoculation of TYRV-t with other Tospovirus spp. or nonrelated viruses did not result in resistance breaking, indicating that the rescuing activity of NSs—though based on suppressing RNA silencing—is species-dependent. |
| first_indexed | 2024-12-18T06:09:39Z |
| format | Article |
| id | doaj.art-a763cedd2eac4dfc836d86b48b2c444a |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-18T06:09:39Z |
| publishDate | 2009-10-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-a763cedd2eac4dfc836d86b48b2c444a2022-12-21T21:18:27ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062009-10-0122101250125710.1094/MPMI-22-10-1250RNAi-Mediated Transgenic Tospovirus Resistance Broken by Intraspecies Silencing Suppressor Protein ComplementationAfshin Hassani-MehrabanA. B. BrenkmanN. J. F. van den BroekRob GoldbachRichard KormelinkExtension of an inverted repeat transgene cassette, containing partial nucleoprotein (N) gene sequences from four different tomato-infecting Tospovirus spp. with a partial N gene sequence from the tomato strain of Tomato yellow ring virus (TYRV-t), renders transgenic Nicotiana benthamiana plants additionally resistant to this strain but not to the soybean strain of this Tospovirus sp. (TYRV-s), both strains exhibiting 14.4% nucleotide sequence divergence in their N genes. Surprisingly, coinoculation of the TYRV-t-resistant transgenic lines with both TYRV-t and TYRV-s resulted in rescue of the former. Mass-spectrometric analysis of the viral ribonucleocapsids accumulating in the transgenic plants showed the presence of the N proteins of both strains excluding hetero-encapsidation as rescue mechanism and indicating suppression of TYRV-t N gene transcript breakdown by RNA interference. Prior (Potato virus X [PVX]-vector-mediated) expression of the TYRV-s silencing suppressor (NSs) gene also allowed TYRV-t to break the resistance. This phenomenon was also observed when the homologous (TYRV-t) NSs gene was provided from a PVX replicon, demonstrating that TYRV can break RNA-mediated host resistance upon a priori expression of its NSs protein. Remarkably, mixed inoculation of TYRV-t with other Tospovirus spp. or nonrelated viruses did not result in resistance breaking, indicating that the rescuing activity of NSs—though based on suppressing RNA silencing—is species-dependent.https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-10-1250 |
| spellingShingle | Afshin Hassani-Mehraban A. B. Brenkman N. J. F. van den Broek Rob Goldbach Richard Kormelink RNAi-Mediated Transgenic Tospovirus Resistance Broken by Intraspecies Silencing Suppressor Protein Complementation Molecular Plant-Microbe Interactions |
| title | RNAi-Mediated Transgenic Tospovirus Resistance Broken by Intraspecies Silencing Suppressor Protein Complementation |
| title_full | RNAi-Mediated Transgenic Tospovirus Resistance Broken by Intraspecies Silencing Suppressor Protein Complementation |
| title_fullStr | RNAi-Mediated Transgenic Tospovirus Resistance Broken by Intraspecies Silencing Suppressor Protein Complementation |
| title_full_unstemmed | RNAi-Mediated Transgenic Tospovirus Resistance Broken by Intraspecies Silencing Suppressor Protein Complementation |
| title_short | RNAi-Mediated Transgenic Tospovirus Resistance Broken by Intraspecies Silencing Suppressor Protein Complementation |
| title_sort | rnai mediated transgenic tospovirus resistance broken by intraspecies silencing suppressor protein complementation |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-10-1250 |
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