The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks.
We have investigated in vivo the role of the carboxy-terminal domain of the Bacillus subtilis Single-Stranded DNA Binding protein (SSB(Cter)) as a recruitment platform at active chromosomal forks for many proteins of the genome maintenance machineries. We probed this SSB(Cter) interactome using GFP...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2010-12-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3000357?pdf=render |
| _version_ | 1811295240923906048 |
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| author | Audrey Costes François Lecointe Stephen McGovern Sophie Quevillon-Cheruel Patrice Polard |
| author_facet | Audrey Costes François Lecointe Stephen McGovern Sophie Quevillon-Cheruel Patrice Polard |
| author_sort | Audrey Costes |
| collection | DOAJ |
| description | We have investigated in vivo the role of the carboxy-terminal domain of the Bacillus subtilis Single-Stranded DNA Binding protein (SSB(Cter)) as a recruitment platform at active chromosomal forks for many proteins of the genome maintenance machineries. We probed this SSB(Cter) interactome using GFP fusions and by Tap-tag and biochemical analysis. It includes at least 12 proteins. The interactome was previously shown to include PriA, RecG, and RecQ and extended in this study by addition of DnaE, SbcC, RarA, RecJ, RecO, XseA, Ung, YpbB, and YrrC. Targeting of YpbB to active forks appears to depend on RecS, a RecQ paralogue, with which it forms a stable complex. Most of these SSB partners are conserved in bacteria, while others, such as the essential DNA polymerase DnaE, YrrC, and the YpbB/RecS complex, appear to be specific to B. subtilis. SSB(Cter) deletion has a moderate impact on B. subtilis cell growth. However, it markedly affects the efficiency of repair of damaged genomic DNA and arrested replication forks. ssbΔCter mutant cells appear deficient in RecA loading on ssDNA, explaining their inefficiency in triggering the SOS response upon exposure to genotoxic agents. Together, our findings show that the bacterial SSB(Cter) acts as a DNA maintenance hub at active chromosomal forks that secures their propagation along the genome. |
| first_indexed | 2024-04-13T05:29:41Z |
| format | Article |
| id | doaj.art-a769f661d0d74386ac06d2a1fca44ee0 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-13T05:29:41Z |
| publishDate | 2010-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-a769f661d0d74386ac06d2a1fca44ee02022-12-22T03:00:29ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042010-12-01612e100123810.1371/journal.pgen.1001238The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks.Audrey CostesFrançois LecointeStephen McGovernSophie Quevillon-CheruelPatrice PolardWe have investigated in vivo the role of the carboxy-terminal domain of the Bacillus subtilis Single-Stranded DNA Binding protein (SSB(Cter)) as a recruitment platform at active chromosomal forks for many proteins of the genome maintenance machineries. We probed this SSB(Cter) interactome using GFP fusions and by Tap-tag and biochemical analysis. It includes at least 12 proteins. The interactome was previously shown to include PriA, RecG, and RecQ and extended in this study by addition of DnaE, SbcC, RarA, RecJ, RecO, XseA, Ung, YpbB, and YrrC. Targeting of YpbB to active forks appears to depend on RecS, a RecQ paralogue, with which it forms a stable complex. Most of these SSB partners are conserved in bacteria, while others, such as the essential DNA polymerase DnaE, YrrC, and the YpbB/RecS complex, appear to be specific to B. subtilis. SSB(Cter) deletion has a moderate impact on B. subtilis cell growth. However, it markedly affects the efficiency of repair of damaged genomic DNA and arrested replication forks. ssbΔCter mutant cells appear deficient in RecA loading on ssDNA, explaining their inefficiency in triggering the SOS response upon exposure to genotoxic agents. Together, our findings show that the bacterial SSB(Cter) acts as a DNA maintenance hub at active chromosomal forks that secures their propagation along the genome.http://europepmc.org/articles/PMC3000357?pdf=render |
| spellingShingle | Audrey Costes François Lecointe Stephen McGovern Sophie Quevillon-Cheruel Patrice Polard The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks. PLoS Genetics |
| title | The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks. |
| title_full | The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks. |
| title_fullStr | The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks. |
| title_full_unstemmed | The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks. |
| title_short | The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks. |
| title_sort | c terminal domain of the bacterial ssb protein acts as a dna maintenance hub at active chromosome replication forks |
| url | http://europepmc.org/articles/PMC3000357?pdf=render |
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