Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro
Oocyte ageing is a complex of processes that occur when matured in vitro oocytes are, after reaching the metaphase II stage, exposed to further in vitro culture. Aged oocytes remaining at the metaphase II stage undergo spontaneous parthenogenetic activation, or cellular death, through apoptosis (fra...
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Czech Academy of Agricultural Sciences
2013-12-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201312-0002_inhibition-of-c-jun-n-terminal-kinase-jnk-suppresses-porcine-oocyte-ageing-in-vitro.php |
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| author | M. Sedmíková J. Petr A. Dörflerová J. Nevoral B. Novotná T. Krejčová E. Chmelíková L. Tůmová |
| author_facet | M. Sedmíková J. Petr A. Dörflerová J. Nevoral B. Novotná T. Krejčová E. Chmelíková L. Tůmová |
| author_sort | M. Sedmíková |
| collection | DOAJ |
| description | Oocyte ageing is a complex of processes that occur when matured in vitro oocytes are, after reaching the metaphase II stage, exposed to further in vitro culture. Aged oocytes remaining at the metaphase II stage undergo spontaneous parthenogenetic activation, or cellular death, through apoptosis (fragmentation) or lysis. The key factor in apoptotic pathway regulation is c-Jun-N-terminal kinase (JNK), stress kinase from the mitogene-activated protein kinase (MAPK) family. To investigate the effect of JNK inhibition on porcine oocytes ageing, cleavage rate, and embryonic development after parthenogenetic activation, DNA fragmentation, and pro-apoptotic factor Bax expression, we cultured in vitro matured oocytes for another 1-4 days in the presence of a JNK inhibitor. The inhibition of JNK significantly protected the oocytes from fragmentation (0% of fragmented oocytes under JNK inhibition vs. 13.4% of fragmented oocytes in the control group, 2nd day of ageing) and increased the percentage of parthenogenetically activated oocytes (82 vs 57.7%, 2nd day of ageing). The embryonic development of oocytes parthenogenetically activated after 24 h of ageing was influenced by JNK inhibition as well. The percentage of oocytes at the morula stage, after seven days of cultivation, was significantly increased when oocytes aged in the presence of a JNK inhibitor (42.5%) by comparison to the percentage of oocytes exposed to ageing in an inhibitor-free medium (23.3%). DNA fragmentation was significantly suppressed by JNK inhibition from the 1st day of ageing, but the expression of pro-apoptotic factor Bax in the oocytes was not influenced. On the basis of our experiments, we can conclude that JNK inhibition suppresses apoptosis and DNA fragmentation of aged oocytes and improves their embryonic development following the parthenogenetic activation. However, to completely eliminate all ageing related processes is insufficient. |
| first_indexed | 2024-04-10T08:26:25Z |
| format | Article |
| id | doaj.art-a76c136f90de46119de51c9f1b3eff95 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:26:25Z |
| publishDate | 2013-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-a76c136f90de46119de51c9f1b3eff952023-02-23T03:33:06ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092013-12-01581253554510.17221/7088-CJAScjs-201312-0002Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitroM. Sedmíková0J. Petr1A. Dörflerová2J. Nevoral3B. Novotná4T. Krejčová5E. Chmelíková6L. Tůmová7Department of Veterinary Sciences, Czech University of Life Sciences Prague, Prague, Czech RepublicInstitute of Animal Science, Prague-Uhříněves, Czech RepublicDepartment of Veterinary Sciences, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Veterinary Sciences, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech RepublicDepartment of Veterinary Sciences, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Veterinary Sciences, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Veterinary Sciences, Czech University of Life Sciences Prague, Prague, Czech RepublicOocyte ageing is a complex of processes that occur when matured in vitro oocytes are, after reaching the metaphase II stage, exposed to further in vitro culture. Aged oocytes remaining at the metaphase II stage undergo spontaneous parthenogenetic activation, or cellular death, through apoptosis (fragmentation) or lysis. The key factor in apoptotic pathway regulation is c-Jun-N-terminal kinase (JNK), stress kinase from the mitogene-activated protein kinase (MAPK) family. To investigate the effect of JNK inhibition on porcine oocytes ageing, cleavage rate, and embryonic development after parthenogenetic activation, DNA fragmentation, and pro-apoptotic factor Bax expression, we cultured in vitro matured oocytes for another 1-4 days in the presence of a JNK inhibitor. The inhibition of JNK significantly protected the oocytes from fragmentation (0% of fragmented oocytes under JNK inhibition vs. 13.4% of fragmented oocytes in the control group, 2nd day of ageing) and increased the percentage of parthenogenetically activated oocytes (82 vs 57.7%, 2nd day of ageing). The embryonic development of oocytes parthenogenetically activated after 24 h of ageing was influenced by JNK inhibition as well. The percentage of oocytes at the morula stage, after seven days of cultivation, was significantly increased when oocytes aged in the presence of a JNK inhibitor (42.5%) by comparison to the percentage of oocytes exposed to ageing in an inhibitor-free medium (23.3%). DNA fragmentation was significantly suppressed by JNK inhibition from the 1st day of ageing, but the expression of pro-apoptotic factor Bax in the oocytes was not influenced. On the basis of our experiments, we can conclude that JNK inhibition suppresses apoptosis and DNA fragmentation of aged oocytes and improves their embryonic development following the parthenogenetic activation. However, to completely eliminate all ageing related processes is insufficient.https://cjas.agriculturejournals.cz/artkey/cjs-201312-0002_inhibition-of-c-jun-n-terminal-kinase-jnk-suppresses-porcine-oocyte-ageing-in-vitro.phpmapkdna fragmentationapoptosisbax |
| spellingShingle | M. Sedmíková J. Petr A. Dörflerová J. Nevoral B. Novotná T. Krejčová E. Chmelíková L. Tůmová Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro Czech Journal of Animal Science mapk dna fragmentation apoptosis bax |
| title | Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro |
| title_full | Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro |
| title_fullStr | Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro |
| title_full_unstemmed | Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro |
| title_short | Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro |
| title_sort | inhibition of c jun n terminal kinase jnk suppresses porcine oocyte ageing in vitro |
| topic | mapk dna fragmentation apoptosis bax |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201312-0002_inhibition-of-c-jun-n-terminal-kinase-jnk-suppresses-porcine-oocyte-ageing-in-vitro.php |
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