Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130)
In 2020, three articles were published on a protein that can activate the immune system by binding to macrophage-inducible C-type lectin receptor (Mincle). In the articles, the protein was referred to as ‘SAP130, a subunit of the histone deacetylase complex.’ However, the Mincle ligand the authors a...
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| Jezik: | English |
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MDPI AG
2021-03-01
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| Serija: | Cells |
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| Online pristup: | https://www.mdpi.com/2073-4409/10/3/590 |
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| author | Paula I. Metselaar Celine Hos Olaf Welting Jos A. Bosch Aletta D. Kraneveld Wouter J. de Jonge Anje A. Te Velde |
| author_facet | Paula I. Metselaar Celine Hos Olaf Welting Jos A. Bosch Aletta D. Kraneveld Wouter J. de Jonge Anje A. Te Velde |
| author_sort | Paula I. Metselaar |
| collection | DOAJ |
| description | In 2020, three articles were published on a protein that can activate the immune system by binding to macrophage-inducible C-type lectin receptor (Mincle). In the articles, the protein was referred to as ‘SAP130, a subunit of the histone deacetylase complex.’ However, the Mincle ligand the authors aimed to investigate is splicing factor 3b subunit 3 (SF3B3). This splicing factor is unrelated to SAP130 (Sin3A associated protein 130, a subunit of the histone deacetylase-dependent Sin3A corepressor complex). The conclusions in the three articles were formulated for SF3B3, while the researchers used qPCR primers and antibodies against SAP130. We retraced the origins of the ambiguity about the two proteins and found that Online Mendelian Inheritance in Man (OMIM) added a Nature publication on SF3B3 as a reference for Sin3A associated protein 130 in 2016. Subsequently, companies such as Abcam referred to OMIM and the Nature article in their products for both SF3B3 and SAP130. In turn, the mistake by OMIM followed in the persistent and confusing use of ‘SAP130′ (spliceosome-associated protein 130) as an alternative symbol for SF3B3. With this report, we aim to eliminate the persistent confusion and separate the literature regarding the two proteins. |
| first_indexed | 2024-03-09T05:04:19Z |
| format | Article |
| id | doaj.art-a76c8d5f830c42408ee9828fe23ff9d0 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T05:04:19Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-a76c8d5f830c42408ee9828fe23ff9d02023-12-03T12:57:01ZengMDPI AGCells2073-44092021-03-0110359010.3390/cells10030590Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130)Paula I. Metselaar0Celine Hos1Olaf Welting2Jos A. Bosch3Aletta D. Kraneveld4Wouter J. de Jonge5Anje A. Te Velde6Tytgat Institute for Liver and Intestinal Research, AGEM, Amsterdam UMC, University of Amsterdam, 1105BK Amsterdam, The NetherlandsTytgat Institute for Liver and Intestinal Research, AGEM, Amsterdam UMC, University of Amsterdam, 1105BK Amsterdam, The NetherlandsTytgat Institute for Liver and Intestinal Research, AGEM, Amsterdam UMC, University of Amsterdam, 1105BK Amsterdam, The NetherlandsDepartment of Psychology, University of Amsterdam, 1018WS Amsterdam, The NetherlandsDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584CG Utrecht, The NetherlandsTytgat Institute for Liver and Intestinal Research, AGEM, Amsterdam UMC, University of Amsterdam, 1105BK Amsterdam, The NetherlandsTytgat Institute for Liver and Intestinal Research, AGEM, Amsterdam UMC, University of Amsterdam, 1105BK Amsterdam, The NetherlandsIn 2020, three articles were published on a protein that can activate the immune system by binding to macrophage-inducible C-type lectin receptor (Mincle). In the articles, the protein was referred to as ‘SAP130, a subunit of the histone deacetylase complex.’ However, the Mincle ligand the authors aimed to investigate is splicing factor 3b subunit 3 (SF3B3). This splicing factor is unrelated to SAP130 (Sin3A associated protein 130, a subunit of the histone deacetylase-dependent Sin3A corepressor complex). The conclusions in the three articles were formulated for SF3B3, while the researchers used qPCR primers and antibodies against SAP130. We retraced the origins of the ambiguity about the two proteins and found that Online Mendelian Inheritance in Man (OMIM) added a Nature publication on SF3B3 as a reference for Sin3A associated protein 130 in 2016. Subsequently, companies such as Abcam referred to OMIM and the Nature article in their products for both SF3B3 and SAP130. In turn, the mistake by OMIM followed in the persistent and confusing use of ‘SAP130′ (spliceosome-associated protein 130) as an alternative symbol for SF3B3. With this report, we aim to eliminate the persistent confusion and separate the literature regarding the two proteins.https://www.mdpi.com/2073-4409/10/3/590SF3B3SAP130splicing factor 3b subunit 3Sin3A associated protein 130spliceosome-associated protein 130 |
| spellingShingle | Paula I. Metselaar Celine Hos Olaf Welting Jos A. Bosch Aletta D. Kraneveld Wouter J. de Jonge Anje A. Te Velde Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130) Cells SF3B3 SAP130 splicing factor 3b subunit 3 Sin3A associated protein 130 spliceosome-associated protein 130 |
| title | Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130) |
| title_full | Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130) |
| title_fullStr | Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130) |
| title_full_unstemmed | Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130) |
| title_short | Ambiguity about Splicing Factor 3b Subunit 3 (SF3B3) and Sin3A Associated Protein 130 (SAP130) |
| title_sort | ambiguity about splicing factor 3b subunit 3 sf3b3 and sin3a associated protein 130 sap130 |
| topic | SF3B3 SAP130 splicing factor 3b subunit 3 Sin3A associated protein 130 spliceosome-associated protein 130 |
| url | https://www.mdpi.com/2073-4409/10/3/590 |
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