Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells
The health benefits of wheat-derived arabinoxylan, a commonly consumed dietary fiber, have been studied for decades. However, its effect on the gut microenvironment and inflammatory bowel disease remains unclear. The objective of this study was to understand the effect of wheat-derived arabinoxylan...
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MDPI AG
2023-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/7/3079 |
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| author | Seita Chudan Riko Ishibashi Miyu Nishikawa Yoshiaki Tabuchi Yoshinori Nagai Shinichi Ikushiro Yukihiro Furusawa |
| author_facet | Seita Chudan Riko Ishibashi Miyu Nishikawa Yoshiaki Tabuchi Yoshinori Nagai Shinichi Ikushiro Yukihiro Furusawa |
| author_sort | Seita Chudan |
| collection | DOAJ |
| description | The health benefits of wheat-derived arabinoxylan, a commonly consumed dietary fiber, have been studied for decades. However, its effect on the gut microenvironment and inflammatory bowel disease remains unclear. The objective of this study was to understand the effect of wheat-derived arabinoxylan on gut microbiota, colonic regulatory T cells (Tregs), and experimental colitis. In this study, healthy and chronic colitis model mice were fed chow containing cellulose or wheat-derived arabinoxylan for 2–6 weeks and subjected to subsequent analysis. A 16S-based metagenomic analysis of the fecal DNA revealed that Lachnospiraceae, comprising butyrate-producing and Treg-inducing bacteria, were overrepresented in arabinoxylan-fed mice. In line with the changes in the gut microbiota, both the fecal butyrate concentration and the colonic Treg population were elevated in the arabinoxylan-fed mice. In a T cell transfer model of chronic colitis, wheat-derived arabinoxylan ameliorated body weight loss and colonic tissue inflammation, which may, in part, be mediated by Treg induction. Moreover, wheat-derived arabinoxylan suppressed TNFα production from type 1 helper T cells in this colitis model. In conclusion, wheat-derived arabinoxylans, by altering the gut microenvironment, may be a promising prebiotic for the prevention of colitis. |
| first_indexed | 2024-03-11T05:29:57Z |
| format | Article |
| id | doaj.art-a76e40256db4401ca8b558471384b8ff |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T05:29:57Z |
| publishDate | 2023-03-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a76e40256db4401ca8b558471384b8ff2023-11-17T17:13:13ZengMDPI AGMolecules1420-30492023-03-01287307910.3390/molecules28073079Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T CellsSeita Chudan0Riko Ishibashi1Miyu Nishikawa2Yoshiaki Tabuchi3Yoshinori Nagai4Shinichi Ikushiro5Yukihiro Furusawa6Department of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Toyama 939-0398, JapanDepartment of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, Toyama 939-0398, JapanDepartment of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Toyama 939-0398, JapanDivision of Molecular Genetics Research, Life Science Research Center, University of Toyama, Toyama 930-0194, JapanDepartment of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, Toyama 939-0398, JapanDepartment of Biotechnology, Faculty of Engineering, Toyama Prefectural University, Toyama 939-0398, JapanDepartment of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, Toyama 939-0398, JapanThe health benefits of wheat-derived arabinoxylan, a commonly consumed dietary fiber, have been studied for decades. However, its effect on the gut microenvironment and inflammatory bowel disease remains unclear. The objective of this study was to understand the effect of wheat-derived arabinoxylan on gut microbiota, colonic regulatory T cells (Tregs), and experimental colitis. In this study, healthy and chronic colitis model mice were fed chow containing cellulose or wheat-derived arabinoxylan for 2–6 weeks and subjected to subsequent analysis. A 16S-based metagenomic analysis of the fecal DNA revealed that Lachnospiraceae, comprising butyrate-producing and Treg-inducing bacteria, were overrepresented in arabinoxylan-fed mice. In line with the changes in the gut microbiota, both the fecal butyrate concentration and the colonic Treg population were elevated in the arabinoxylan-fed mice. In a T cell transfer model of chronic colitis, wheat-derived arabinoxylan ameliorated body weight loss and colonic tissue inflammation, which may, in part, be mediated by Treg induction. Moreover, wheat-derived arabinoxylan suppressed TNFα production from type 1 helper T cells in this colitis model. In conclusion, wheat-derived arabinoxylans, by altering the gut microenvironment, may be a promising prebiotic for the prevention of colitis.https://www.mdpi.com/1420-3049/28/7/3079arabinoxylanwheatgut bacteriaregulatory T cellscolitisshort-chain fatty acid |
| spellingShingle | Seita Chudan Riko Ishibashi Miyu Nishikawa Yoshiaki Tabuchi Yoshinori Nagai Shinichi Ikushiro Yukihiro Furusawa Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells Molecules arabinoxylan wheat gut bacteria regulatory T cells colitis short-chain fatty acid |
| title | Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells |
| title_full | Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells |
| title_fullStr | Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells |
| title_full_unstemmed | Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells |
| title_short | Effect of Wheat-Derived Arabinoxylan on the Gut Microbiota Composition and Colonic Regulatory T Cells |
| title_sort | effect of wheat derived arabinoxylan on the gut microbiota composition and colonic regulatory t cells |
| topic | arabinoxylan wheat gut bacteria regulatory T cells colitis short-chain fatty acid |
| url | https://www.mdpi.com/1420-3049/28/7/3079 |
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