High salt intake damages the heart through activation of cardiac (pro) renin receptors even at an early stage of hypertension.
It has not yet been fully elucidated whether cardiac tissue levels of prorenin, renin and (P)RR are activated in hypertension with a high salt intake. We hypothesized that a high salt intake activates the cardiac tissue renin angiotensin system and prorenin-(pro)renin receptor system, and damages th...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4370564?pdf=render |
| _version_ | 1811321553509416960 |
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| author | Yuka Hayakawa Takuma Aoyama Chiharu Yokoyama Chihiro Okamoto Hisaaki Komaki Shingo Minatoguchi Masamitsu Iwasa Yoshihisa Yamada Itta Kawamura Masanori Kawasaki Kazuhiko Nishigaki Atsushi Mikami Fumiaki Suzuki Shinya Minatoguchi |
| author_facet | Yuka Hayakawa Takuma Aoyama Chiharu Yokoyama Chihiro Okamoto Hisaaki Komaki Shingo Minatoguchi Masamitsu Iwasa Yoshihisa Yamada Itta Kawamura Masanori Kawasaki Kazuhiko Nishigaki Atsushi Mikami Fumiaki Suzuki Shinya Minatoguchi |
| author_sort | Yuka Hayakawa |
| collection | DOAJ |
| description | It has not yet been fully elucidated whether cardiac tissue levels of prorenin, renin and (P)RR are activated in hypertension with a high salt intake. We hypothesized that a high salt intake activates the cardiac tissue renin angiotensin system and prorenin-(pro)renin receptor system, and damages the heart at an early stage of hypertension.Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) received regular (normal-salt diet, 0.9%) and high-salt (8.9%) chow for 6 weeks from 6 to 12 weeks of age. The systolic blood pressure, plasma renin activity (PRA) and plasma angiotensin II concentration were measured, and the protein expressions of prorenin, (pro)renin receptor, angiotensinogen, angiotensin II AT1 receptor, ERK1/2, TGF-β, p38MAPK and HSP27 in the myocardium were investigated. The cardiac function was assessed by echocardiography, and histological analysis of the myocardium was performed.The high-salt diet significantly increased the systolic blood pressure, and significantly reduced the PRA and plasma angiotensin II concentration both in the WKYs and SHRs. Cardiac expressions of prorenin, renin, (P)RR, angiotensinogen, angiotensin II AT1 receptor, phosphorylated (p)-ERK1/2, p-p38MAPK, TGF-β and p-HSP27 were significantly increased by the high salt diet both in the WKYs and SHRs. The high-salt diet significantly increased the interventricular septum thickness and cardiomyocyte size, and accelerated cardiac interstitial and perivascular fibrosis both in the WKYs and SHRs. On the other hand, dilatation of left ventricular end-diastolic dimension and impairment of left ventricular fractional shortening was shown only in salt loaded SHRs.The high-salt diet markedly accelerated cardiac damage through the stimulation of cardiac (P)RR and angiotensin II AT1 receptor by increasing tissue prorenin, renin and angiotensinogen and the activation of ERK1/2, TGF-β, p38MAPK and HSP27 under higher blood pressure. |
| first_indexed | 2024-04-13T13:20:00Z |
| format | Article |
| id | doaj.art-a770047c67a040a4bd57542b822e1846 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T13:20:00Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a770047c67a040a4bd57542b822e18462022-12-22T02:45:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e012045310.1371/journal.pone.0120453High salt intake damages the heart through activation of cardiac (pro) renin receptors even at an early stage of hypertension.Yuka HayakawaTakuma AoyamaChiharu YokoyamaChihiro OkamotoHisaaki KomakiShingo MinatoguchiMasamitsu IwasaYoshihisa YamadaItta KawamuraMasanori KawasakiKazuhiko NishigakiAtsushi MikamiFumiaki SuzukiShinya MinatoguchiIt has not yet been fully elucidated whether cardiac tissue levels of prorenin, renin and (P)RR are activated in hypertension with a high salt intake. We hypothesized that a high salt intake activates the cardiac tissue renin angiotensin system and prorenin-(pro)renin receptor system, and damages the heart at an early stage of hypertension.Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) received regular (normal-salt diet, 0.9%) and high-salt (8.9%) chow for 6 weeks from 6 to 12 weeks of age. The systolic blood pressure, plasma renin activity (PRA) and plasma angiotensin II concentration were measured, and the protein expressions of prorenin, (pro)renin receptor, angiotensinogen, angiotensin II AT1 receptor, ERK1/2, TGF-β, p38MAPK and HSP27 in the myocardium were investigated. The cardiac function was assessed by echocardiography, and histological analysis of the myocardium was performed.The high-salt diet significantly increased the systolic blood pressure, and significantly reduced the PRA and plasma angiotensin II concentration both in the WKYs and SHRs. Cardiac expressions of prorenin, renin, (P)RR, angiotensinogen, angiotensin II AT1 receptor, phosphorylated (p)-ERK1/2, p-p38MAPK, TGF-β and p-HSP27 were significantly increased by the high salt diet both in the WKYs and SHRs. The high-salt diet significantly increased the interventricular septum thickness and cardiomyocyte size, and accelerated cardiac interstitial and perivascular fibrosis both in the WKYs and SHRs. On the other hand, dilatation of left ventricular end-diastolic dimension and impairment of left ventricular fractional shortening was shown only in salt loaded SHRs.The high-salt diet markedly accelerated cardiac damage through the stimulation of cardiac (P)RR and angiotensin II AT1 receptor by increasing tissue prorenin, renin and angiotensinogen and the activation of ERK1/2, TGF-β, p38MAPK and HSP27 under higher blood pressure.http://europepmc.org/articles/PMC4370564?pdf=render |
| spellingShingle | Yuka Hayakawa Takuma Aoyama Chiharu Yokoyama Chihiro Okamoto Hisaaki Komaki Shingo Minatoguchi Masamitsu Iwasa Yoshihisa Yamada Itta Kawamura Masanori Kawasaki Kazuhiko Nishigaki Atsushi Mikami Fumiaki Suzuki Shinya Minatoguchi High salt intake damages the heart through activation of cardiac (pro) renin receptors even at an early stage of hypertension. PLoS ONE |
| title | High salt intake damages the heart through activation of cardiac (pro) renin receptors even at an early stage of hypertension. |
| title_full | High salt intake damages the heart through activation of cardiac (pro) renin receptors even at an early stage of hypertension. |
| title_fullStr | High salt intake damages the heart through activation of cardiac (pro) renin receptors even at an early stage of hypertension. |
| title_full_unstemmed | High salt intake damages the heart through activation of cardiac (pro) renin receptors even at an early stage of hypertension. |
| title_short | High salt intake damages the heart through activation of cardiac (pro) renin receptors even at an early stage of hypertension. |
| title_sort | high salt intake damages the heart through activation of cardiac pro renin receptors even at an early stage of hypertension |
| url | http://europepmc.org/articles/PMC4370564?pdf=render |
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