Periodontitis relates to benign prostatic hyperplasia via the gut microbiota and fecal metabolome
ObjectivesPeriodontitis is associated with benign prostatic hyperplasia (BPH), whether it related to gut floramicrobiota and metabonomics is unclear.MethodsWe established ligature-induced periodontitis (EP), testosterone-induced BPH, and composite rat models. Fecal samples were collected to detect g...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-12-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1280628/full |
| _version_ | 1797392817916477440 |
|---|---|
| author | Xing-Pei Guo Xing-Pei Guo Jun Yang Lan Wu Lan Wu Cheng Fang Jia-Min Gu Jia-Min Gu Fei Li Fei Li Han-Song Liu Lu-Yao Li Lu-Yao Li Shuang-Ying Wang |
| author_facet | Xing-Pei Guo Xing-Pei Guo Jun Yang Lan Wu Lan Wu Cheng Fang Jia-Min Gu Jia-Min Gu Fei Li Fei Li Han-Song Liu Lu-Yao Li Lu-Yao Li Shuang-Ying Wang |
| author_sort | Xing-Pei Guo |
| collection | DOAJ |
| description | ObjectivesPeriodontitis is associated with benign prostatic hyperplasia (BPH), whether it related to gut floramicrobiota and metabonomics is unclear.MethodsWe established ligature-induced periodontitis (EP), testosterone-induced BPH, and composite rat models. Fecal samples were collected to detect gut microbiota by 16S rDNA sequencing and metabonomics were detected by liquid chromatography tandem mass spectrometry (LC-MS/MS).ResultsSequencing results revealed differential gut floramicrobiota composition between EP+BPH group and other three groups. The abundances of Ruminococcus flavefaciens were significantly increased in EP+BPH group compared with other groups. Tenericutes, Mollicutes, RF39 and Ruminococcus gnavus were significantly decreased in EP+BPH group compared with BPH group, while Ruminococcus callidus and Escherichia were significantly decreased compared with EP group. For gut metabonomics, LC-MS/MS showed that fecal metabolites and seven metabolic pathways were changed in EP+BPH group, such as biosynthesis of unsaturated fatty acids, steroid hormone biosynthesis. Correlation analysis showed that the alterations of gut metabolism were significantly correlated with differential gut floramicrobiota, such as Ruminococcus callidus and Ruminococcus flavefaciens.ConclusionOur study highlights the relationship of periodontitis and BPH, the alterations of gut floramicrobiota and metabolites may be involved in two diseases, which provides new idea for prevention and treatment of patients with periodontitis concurrent BPH. |
| first_indexed | 2024-03-08T23:53:00Z |
| format | Article |
| id | doaj.art-a7754a50638748b6bf907d393b7252fc |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T23:53:00Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a7754a50638748b6bf907d393b7252fc2023-12-13T09:56:19ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-12-011410.3389/fmicb.2023.12806281280628Periodontitis relates to benign prostatic hyperplasia via the gut microbiota and fecal metabolomeXing-Pei Guo0Xing-Pei Guo1Jun Yang2Lan Wu3Lan Wu4Cheng Fang5Jia-Min Gu6Jia-Min Gu7Fei Li8Fei Li9Han-Song Liu10Lu-Yao Li11Lu-Yao Li12Shuang-Ying Wang13Department of General Surgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan, ChinaCenter for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Urology, The First People's Hospital of Tianmen in Hubei Province, The Affiliated Hospital of Hubei University of Science and Technology, Tianmen, ChinaCenter for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Stomatology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaCenter for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaCenter for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Urology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaCenter for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Urology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of General Surgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan, ChinaCenter for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Urology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaCenter for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan, ChinaObjectivesPeriodontitis is associated with benign prostatic hyperplasia (BPH), whether it related to gut floramicrobiota and metabonomics is unclear.MethodsWe established ligature-induced periodontitis (EP), testosterone-induced BPH, and composite rat models. Fecal samples were collected to detect gut microbiota by 16S rDNA sequencing and metabonomics were detected by liquid chromatography tandem mass spectrometry (LC-MS/MS).ResultsSequencing results revealed differential gut floramicrobiota composition between EP+BPH group and other three groups. The abundances of Ruminococcus flavefaciens were significantly increased in EP+BPH group compared with other groups. Tenericutes, Mollicutes, RF39 and Ruminococcus gnavus were significantly decreased in EP+BPH group compared with BPH group, while Ruminococcus callidus and Escherichia were significantly decreased compared with EP group. For gut metabonomics, LC-MS/MS showed that fecal metabolites and seven metabolic pathways were changed in EP+BPH group, such as biosynthesis of unsaturated fatty acids, steroid hormone biosynthesis. Correlation analysis showed that the alterations of gut metabolism were significantly correlated with differential gut floramicrobiota, such as Ruminococcus callidus and Ruminococcus flavefaciens.ConclusionOur study highlights the relationship of periodontitis and BPH, the alterations of gut floramicrobiota and metabolites may be involved in two diseases, which provides new idea for prevention and treatment of patients with periodontitis concurrent BPH.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1280628/fullperiodontitisbenign prostatic hyperplasiagut microbiotafecal metabolitesmulti-omics analysis |
| spellingShingle | Xing-Pei Guo Xing-Pei Guo Jun Yang Lan Wu Lan Wu Cheng Fang Jia-Min Gu Jia-Min Gu Fei Li Fei Li Han-Song Liu Lu-Yao Li Lu-Yao Li Shuang-Ying Wang Periodontitis relates to benign prostatic hyperplasia via the gut microbiota and fecal metabolome Frontiers in Microbiology periodontitis benign prostatic hyperplasia gut microbiota fecal metabolites multi-omics analysis |
| title | Periodontitis relates to benign prostatic hyperplasia via the gut microbiota and fecal metabolome |
| title_full | Periodontitis relates to benign prostatic hyperplasia via the gut microbiota and fecal metabolome |
| title_fullStr | Periodontitis relates to benign prostatic hyperplasia via the gut microbiota and fecal metabolome |
| title_full_unstemmed | Periodontitis relates to benign prostatic hyperplasia via the gut microbiota and fecal metabolome |
| title_short | Periodontitis relates to benign prostatic hyperplasia via the gut microbiota and fecal metabolome |
| title_sort | periodontitis relates to benign prostatic hyperplasia via the gut microbiota and fecal metabolome |
| topic | periodontitis benign prostatic hyperplasia gut microbiota fecal metabolites multi-omics analysis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1280628/full |
| work_keys_str_mv | AT xingpeiguo periodontitisrelatestobenignprostatichyperplasiaviathegutmicrobiotaandfecalmetabolome AT xingpeiguo periodontitisrelatestobenignprostatichyperplasiaviathegutmicrobiotaandfecalmetabolome AT junyang periodontitisrelatestobenignprostatichyperplasiaviathegutmicrobiotaandfecalmetabolome AT lanwu periodontitisrelatestobenignprostatichyperplasiaviathegutmicrobiotaandfecalmetabolome AT lanwu periodontitisrelatestobenignprostatichyperplasiaviathegutmicrobiotaandfecalmetabolome AT chengfang periodontitisrelatestobenignprostatichyperplasiaviathegutmicrobiotaandfecalmetabolome AT jiamingu periodontitisrelatestobenignprostatichyperplasiaviathegutmicrobiotaandfecalmetabolome AT jiamingu periodontitisrelatestobenignprostatichyperplasiaviathegutmicrobiotaandfecalmetabolome AT feili periodontitisrelatestobenignprostatichyperplasiaviathegutmicrobiotaandfecalmetabolome AT feili periodontitisrelatestobenignprostatichyperplasiaviathegutmicrobiotaandfecalmetabolome AT hansongliu periodontitisrelatestobenignprostatichyperplasiaviathegutmicrobiotaandfecalmetabolome AT luyaoli periodontitisrelatestobenignprostatichyperplasiaviathegutmicrobiotaandfecalmetabolome AT luyaoli periodontitisrelatestobenignprostatichyperplasiaviathegutmicrobiotaandfecalmetabolome AT shuangyingwang periodontitisrelatestobenignprostatichyperplasiaviathegutmicrobiotaandfecalmetabolome |