Anticancer Effects of Propolis Extracts Obtained Using the Cold Separation Method on Breast Cancer Cell Lines
Propolis and its extracts show a wide spectrum of biological activity. Due to the necessity to use high temperatures and high polarity in the eluent, the obtained extracts are depleted of active compounds. The new, cold separation method allows obtaining a qualitatively better product containing a n...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/4/884 |
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| author | Marek Gogacz Jerzy Peszke Dorota Natorska-Chomicka Monika Ruszała Katarzyna Dos Santos Szewczyk |
| author_facet | Marek Gogacz Jerzy Peszke Dorota Natorska-Chomicka Monika Ruszała Katarzyna Dos Santos Szewczyk |
| author_sort | Marek Gogacz |
| collection | DOAJ |
| description | Propolis and its extracts show a wide spectrum of biological activity. Due to the necessity to use high temperatures and high polarity in the eluent, the obtained extracts are depleted of active compounds. The new, cold separation method allows obtaining a qualitatively better product containing a number of chemical compounds absent in extracts obtained using high-temperature methods. The purpose of our study was to evaluate the biological activity of propolis extracts produced with the cold separation method in four female breast cancer cell lines: MDA-MB-231, MDA-MB-468, MCF-7, and T-47D. The results of the breast cancer cell viability were obtained using the MTT test. Propolis extracts at 75 and 80% showed similar cytotoxicity against cancer cells, with the polyphenol fraction 75% being slightly more negative for cells. Propolis extracts at concentrations of 50, 75, and 100 µg/mL significantly reduced cell viability. With the exception of the MDA-MB-231 line, cell viability was also decreased after incubation with a concentration of 25 µg/mL. Our results suggest that propolis extracts obtained with the cold separation method may be considered as promising compounds for the production of health-promoting supplements. |
| first_indexed | 2024-03-11T08:15:04Z |
| format | Article |
| id | doaj.art-a775d60afa444946ae81f3f4dd111f53 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T08:15:04Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-a775d60afa444946ae81f3f4dd111f532023-11-16T22:48:47ZengMDPI AGPlants2223-77472023-02-0112488410.3390/plants12040884Anticancer Effects of Propolis Extracts Obtained Using the Cold Separation Method on Breast Cancer Cell LinesMarek Gogacz0Jerzy Peszke1Dorota Natorska-Chomicka2Monika Ruszała3Katarzyna Dos Santos Szewczyk4Chair and Department of Gynecology, Medical University of Lublin, 20-090 Lublin, PolandDepartment of Experimental Biotechnology, Decont LLC, 08-500 Ryki, PolandChair and Department of Toxicology, Faculty of Pharmacy, Medical University of Lublin, 20-090 Lublin, PolandChair and Department of Obstetrics and Perinatology, Medical University of Lublin, 20-090 Lublin, PolandDepartment of Pharmaceutical Botany, Medical University of Lublin, 20-093 Lublin, PolandPropolis and its extracts show a wide spectrum of biological activity. Due to the necessity to use high temperatures and high polarity in the eluent, the obtained extracts are depleted of active compounds. The new, cold separation method allows obtaining a qualitatively better product containing a number of chemical compounds absent in extracts obtained using high-temperature methods. The purpose of our study was to evaluate the biological activity of propolis extracts produced with the cold separation method in four female breast cancer cell lines: MDA-MB-231, MDA-MB-468, MCF-7, and T-47D. The results of the breast cancer cell viability were obtained using the MTT test. Propolis extracts at 75 and 80% showed similar cytotoxicity against cancer cells, with the polyphenol fraction 75% being slightly more negative for cells. Propolis extracts at concentrations of 50, 75, and 100 µg/mL significantly reduced cell viability. With the exception of the MDA-MB-231 line, cell viability was also decreased after incubation with a concentration of 25 µg/mL. Our results suggest that propolis extracts obtained with the cold separation method may be considered as promising compounds for the production of health-promoting supplements.https://www.mdpi.com/2223-7747/12/4/884propoliscold separationbreast cancer |
| spellingShingle | Marek Gogacz Jerzy Peszke Dorota Natorska-Chomicka Monika Ruszała Katarzyna Dos Santos Szewczyk Anticancer Effects of Propolis Extracts Obtained Using the Cold Separation Method on Breast Cancer Cell Lines Plants propolis cold separation breast cancer |
| title | Anticancer Effects of Propolis Extracts Obtained Using the Cold Separation Method on Breast Cancer Cell Lines |
| title_full | Anticancer Effects of Propolis Extracts Obtained Using the Cold Separation Method on Breast Cancer Cell Lines |
| title_fullStr | Anticancer Effects of Propolis Extracts Obtained Using the Cold Separation Method on Breast Cancer Cell Lines |
| title_full_unstemmed | Anticancer Effects of Propolis Extracts Obtained Using the Cold Separation Method on Breast Cancer Cell Lines |
| title_short | Anticancer Effects of Propolis Extracts Obtained Using the Cold Separation Method on Breast Cancer Cell Lines |
| title_sort | anticancer effects of propolis extracts obtained using the cold separation method on breast cancer cell lines |
| topic | propolis cold separation breast cancer |
| url | https://www.mdpi.com/2223-7747/12/4/884 |
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