Improvement of vaginal probiotics Lactobacillus crispatus on intrauterine adhesion in mice model and in clinical practice
Abstract Background Intrauterine adhesion (IUA) is a frequent acquired endometrial condition, for which there is no effective preventive or treatment. Previous studies have found that vaginal microbiota dysregulation is closely related to endometrial fibrosis and IUA. Therefore, we wondered whether...
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| Format: | Article |
| Language: | English |
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BMC
2023-03-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-02823-y |
| _version_ | 1797865425878384640 |
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| author | Fei Wu Yao Kong Wenjie Chen Dingfa Liang Qin Xiao Lijuan Hu Xiao Tan Jing Wei Yujuan Liu Xiaorong Deng Zhaoxia Liu Tingtao Chen |
| author_facet | Fei Wu Yao Kong Wenjie Chen Dingfa Liang Qin Xiao Lijuan Hu Xiao Tan Jing Wei Yujuan Liu Xiaorong Deng Zhaoxia Liu Tingtao Chen |
| author_sort | Fei Wu |
| collection | DOAJ |
| description | Abstract Background Intrauterine adhesion (IUA) is a frequent acquired endometrial condition, for which there is no effective preventive or treatment. Previous studies have found that vaginal microbiota dysregulation is closely related to endometrial fibrosis and IUA. Therefore, we wondered whether restoration of vaginal microbiota by vaginal administration of L. crispatus could prevent endometrial fibrosis and ameliorate IUA. Results First, we created a mechanically injured mouse model of IUA and restored the mice’s vaginal microbiota by the addition of L. crispatus convolvulus. The observations suggested that intrauterine injections of L. crispatus significantly decreased the degree of uterine fibrosis, the levels of IL-1β and TNF-α in blood, and downregulated the TGF-β1/SMADs signaling pathway in IUA mice. A therapy with L. crispatus considerably raised the abundance of the helpful bacteria Lactobacillus and Oscillospira and restored the balance of the vaginal microbiota in IUA mice, according to high-throughput sequencing. Then we conducted a randomized controlled trial to compare the therapeutic effect of L. crispatus with estrogen after transcervical resection of adhesion (TCRA). And the results showed that vaginal probiotics had a better potential to prevent intrauterine adhesion than estrogen. Conclusions This study confirmed that L. crispatus could restore vaginal microbiota after intrauterine surgery, inhibit endometrial fibrosis, and finally play a preventive and therapeutic role in IUA. At the same time, it is a new exploration for the treatment of gynecological diseases with vaginal probiotics. Clinical trial registration : http://www.chictr.org.cn/ , identifier (ChiCTR1900022522), registration time: 15/04/2019. |
| first_indexed | 2024-04-09T23:08:38Z |
| format | Article |
| id | doaj.art-a776c3dd42c54dadaee4faca76afa115 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-09T23:08:38Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-a776c3dd42c54dadaee4faca76afa1152023-03-22T10:34:20ZengBMCBMC Microbiology1471-21802023-03-0123111310.1186/s12866-023-02823-yImprovement of vaginal probiotics Lactobacillus crispatus on intrauterine adhesion in mice model and in clinical practiceFei Wu0Yao Kong1Wenjie Chen2Dingfa Liang3Qin Xiao4Lijuan Hu5Xiao Tan6Jing Wei7Yujuan Liu8Xiaorong Deng9Zhaoxia Liu10Tingtao Chen11Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang UniversityNational Engineering Research Center for Bioengineering Drugs and The Technologies, Institute of Translational Medicine, Nanchang UniversityDepartment of Obstetrics and Gynecology, The Affiliated Hospital of Jiangxi University of Traditional Chinese MedicineDepartment of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang UniversityAbstract Background Intrauterine adhesion (IUA) is a frequent acquired endometrial condition, for which there is no effective preventive or treatment. Previous studies have found that vaginal microbiota dysregulation is closely related to endometrial fibrosis and IUA. Therefore, we wondered whether restoration of vaginal microbiota by vaginal administration of L. crispatus could prevent endometrial fibrosis and ameliorate IUA. Results First, we created a mechanically injured mouse model of IUA and restored the mice’s vaginal microbiota by the addition of L. crispatus convolvulus. The observations suggested that intrauterine injections of L. crispatus significantly decreased the degree of uterine fibrosis, the levels of IL-1β and TNF-α in blood, and downregulated the TGF-β1/SMADs signaling pathway in IUA mice. A therapy with L. crispatus considerably raised the abundance of the helpful bacteria Lactobacillus and Oscillospira and restored the balance of the vaginal microbiota in IUA mice, according to high-throughput sequencing. Then we conducted a randomized controlled trial to compare the therapeutic effect of L. crispatus with estrogen after transcervical resection of adhesion (TCRA). And the results showed that vaginal probiotics had a better potential to prevent intrauterine adhesion than estrogen. Conclusions This study confirmed that L. crispatus could restore vaginal microbiota after intrauterine surgery, inhibit endometrial fibrosis, and finally play a preventive and therapeutic role in IUA. At the same time, it is a new exploration for the treatment of gynecological diseases with vaginal probiotics. Clinical trial registration : http://www.chictr.org.cn/ , identifier (ChiCTR1900022522), registration time: 15/04/2019.https://doi.org/10.1186/s12866-023-02823-yIntrauterine adhesionLactobacillus crispatusEndometrial injuryHigh-throughput sequencingEstrogen |
| spellingShingle | Fei Wu Yao Kong Wenjie Chen Dingfa Liang Qin Xiao Lijuan Hu Xiao Tan Jing Wei Yujuan Liu Xiaorong Deng Zhaoxia Liu Tingtao Chen Improvement of vaginal probiotics Lactobacillus crispatus on intrauterine adhesion in mice model and in clinical practice BMC Microbiology Intrauterine adhesion Lactobacillus crispatus Endometrial injury High-throughput sequencing Estrogen |
| title | Improvement of vaginal probiotics Lactobacillus crispatus on intrauterine adhesion in mice model and in clinical practice |
| title_full | Improvement of vaginal probiotics Lactobacillus crispatus on intrauterine adhesion in mice model and in clinical practice |
| title_fullStr | Improvement of vaginal probiotics Lactobacillus crispatus on intrauterine adhesion in mice model and in clinical practice |
| title_full_unstemmed | Improvement of vaginal probiotics Lactobacillus crispatus on intrauterine adhesion in mice model and in clinical practice |
| title_short | Improvement of vaginal probiotics Lactobacillus crispatus on intrauterine adhesion in mice model and in clinical practice |
| title_sort | improvement of vaginal probiotics lactobacillus crispatus on intrauterine adhesion in mice model and in clinical practice |
| topic | Intrauterine adhesion Lactobacillus crispatus Endometrial injury High-throughput sequencing Estrogen |
| url | https://doi.org/10.1186/s12866-023-02823-y |
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