Biodegradation of Extractable Petroleum Hydrocarbons by Consortia <i>Bacillus cereus</i> and <i>Pseudomonas putida</i> in Petroleum Contaminated-Soil

Contamination of soil by the activities of exploration, production, and disposal of oil waste into the environment causes serious damage to the environmental ecosystem, the target of processing by the bacteria as a model for remediation of oil contaminated site. Thus, the study was focused on determ...

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Main Authors: Abubakar Tuhuloula, Suprapto Suprapto, Ali Altway, Sri Rachmania Juliastuti
Format: Article
Language:English
Published: Department of Chemistry, Universitas Gadjah Mada 2019-04-01
Series:Indonesian Journal of Chemistry
Subjects:
Online Access:https://jurnal.ugm.ac.id/ijc/article/view/33765
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author Abubakar Tuhuloula
Suprapto Suprapto
Ali Altway
Sri Rachmania Juliastuti
author_facet Abubakar Tuhuloula
Suprapto Suprapto
Ali Altway
Sri Rachmania Juliastuti
author_sort Abubakar Tuhuloula
collection DOAJ
description Contamination of soil by the activities of exploration, production, and disposal of oil waste into the environment causes serious damage to the environmental ecosystem, the target of processing by the bacteria as a model for remediation of oil contaminated site. Thus, the study was focused on determining the biodegradation percentage of extractable petroleum hydrocarbons as a function of the oil concentration. This research was conducted in a slurry bioreactor with mixed contaminated soil to water ratio of 20:80 (wt.%). A consortium of Bacillus cereus and Pseudomonas putida bacteria 10% (v/v) and 15% (v/v) with the ratio of 2:3, 1:1, and 3:2 was inserted into the slurry bioreactor and a single reactor was used as a control. The result of identification with an initial concentration of extractable petroleum hydrocarbons of 299.53 ng/µL, after 49 days of incubation for bacterial consortium 10% (v/v), the concentration was reduced to 85.31; 32.43; 59.74; and 112.22 ng/µL respectively and the biodegradation percentage was 71.5; 89.17; 80.05; and 62.54%. As for the bacterial consortium concentration of 15% (v/v) with the same ratio and control, the effluent concentration was 12.48; 7.72; 18.93 ng/µL, respectively or the biodegradation percentage was 95.83; 97.42; 93.68%.
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spelling doaj.art-a777b0a506bb4da485507828c5bddbcd2022-12-21T21:37:28ZengDepartment of Chemistry, Universitas Gadjah MadaIndonesian Journal of Chemistry1411-94202460-15782019-04-0119234735510.22146/ijc.3376523224Biodegradation of Extractable Petroleum Hydrocarbons by Consortia <i>Bacillus cereus</i> and <i>Pseudomonas putida</i> in Petroleum Contaminated-SoilAbubakar Tuhuloula0Suprapto Suprapto1Ali Altway2Sri Rachmania Juliastuti3Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya 60111, IndonesiaDepartment of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya 60111, IndonesiaDepartment of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya 60111, IndonesiaDepartment of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya 60111, IndonesiaContamination of soil by the activities of exploration, production, and disposal of oil waste into the environment causes serious damage to the environmental ecosystem, the target of processing by the bacteria as a model for remediation of oil contaminated site. Thus, the study was focused on determining the biodegradation percentage of extractable petroleum hydrocarbons as a function of the oil concentration. This research was conducted in a slurry bioreactor with mixed contaminated soil to water ratio of 20:80 (wt.%). A consortium of Bacillus cereus and Pseudomonas putida bacteria 10% (v/v) and 15% (v/v) with the ratio of 2:3, 1:1, and 3:2 was inserted into the slurry bioreactor and a single reactor was used as a control. The result of identification with an initial concentration of extractable petroleum hydrocarbons of 299.53 ng/µL, after 49 days of incubation for bacterial consortium 10% (v/v), the concentration was reduced to 85.31; 32.43; 59.74; and 112.22 ng/µL respectively and the biodegradation percentage was 71.5; 89.17; 80.05; and 62.54%. As for the bacterial consortium concentration of 15% (v/v) with the same ratio and control, the effluent concentration was 12.48; 7.72; 18.93 ng/µL, respectively or the biodegradation percentage was 95.83; 97.42; 93.68%.https://jurnal.ugm.ac.id/ijc/article/view/33765biodegradationextractable petroleum hydrocarbonsslurry bioreactor
spellingShingle Abubakar Tuhuloula
Suprapto Suprapto
Ali Altway
Sri Rachmania Juliastuti
Biodegradation of Extractable Petroleum Hydrocarbons by Consortia <i>Bacillus cereus</i> and <i>Pseudomonas putida</i> in Petroleum Contaminated-Soil
Indonesian Journal of Chemistry
biodegradation
extractable petroleum hydrocarbons
slurry bioreactor
title Biodegradation of Extractable Petroleum Hydrocarbons by Consortia <i>Bacillus cereus</i> and <i>Pseudomonas putida</i> in Petroleum Contaminated-Soil
title_full Biodegradation of Extractable Petroleum Hydrocarbons by Consortia <i>Bacillus cereus</i> and <i>Pseudomonas putida</i> in Petroleum Contaminated-Soil
title_fullStr Biodegradation of Extractable Petroleum Hydrocarbons by Consortia <i>Bacillus cereus</i> and <i>Pseudomonas putida</i> in Petroleum Contaminated-Soil
title_full_unstemmed Biodegradation of Extractable Petroleum Hydrocarbons by Consortia <i>Bacillus cereus</i> and <i>Pseudomonas putida</i> in Petroleum Contaminated-Soil
title_short Biodegradation of Extractable Petroleum Hydrocarbons by Consortia <i>Bacillus cereus</i> and <i>Pseudomonas putida</i> in Petroleum Contaminated-Soil
title_sort biodegradation of extractable petroleum hydrocarbons by consortia i bacillus cereus i and i pseudomonas putida i in petroleum contaminated soil
topic biodegradation
extractable petroleum hydrocarbons
slurry bioreactor
url https://jurnal.ugm.ac.id/ijc/article/view/33765
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