Three-dimensional electron crystallography of protein microcrystals

We demonstrate that it is feasible to determine high-resolution protein structures by electron crystallography of three-dimensional crystals in an electron cryo-microscope (CryoEM). Lysozyme microcrystals were frozen on an electron microscopy grid, and electron diffraction data collected to 1.7 Å re...

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Bibliographic Details
Main Authors: Dan Shi, Brent L Nannenga, Matthew G Iadanza, Tamir Gonen
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2013-11-01
Series:eLife
Subjects:
Online Access:https://elifesciences.org/articles/01345

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