Environmental stress evaluation of Coffea arabica L. leaves from spectrophotometric fingerprints by PCA and OSC–PLS–DA
The effects of hydric stress and sunlight access conditions on metabolic compounds in coffee leaves were investigated utilizing statistical mixture design extractor solvents. PCA and OSC–PLS–DA chemometric methods were used to analyze UV–visible spectra of irrigated and non-irrigated Coffea arabica...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-12-01
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| Series: | Arabian Journal of Chemistry |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535216300685 |
| _version_ | 1818109660730753024 |
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| author | Guilherme Luiz Scheel Elis Daiane Pauli Miroslava Rakocevic Roy Edward Bruns Ieda Spacino Scarminio |
| author_facet | Guilherme Luiz Scheel Elis Daiane Pauli Miroslava Rakocevic Roy Edward Bruns Ieda Spacino Scarminio |
| author_sort | Guilherme Luiz Scheel |
| collection | DOAJ |
| description | The effects of hydric stress and sunlight access conditions on metabolic compounds in coffee leaves were investigated utilizing statistical mixture design extractor solvents. PCA and OSC–PLS–DA chemometric methods were used to analyze UV–visible spectra of irrigated and non-irrigated Coffea arabica L. leaves from low (<40 cm, self-shaded) and higher (>80 cm, light exposed) strata. The first latent variable of the OSC–PLS–DA score plot perfectly discriminated extracts of 34 calibration and 14 validation samples of irrigated and non-irrigated leaves. Higher spectral signals observed at the 410, 505, 535, 607 and 665 nm wavelengths are attributed to conjugate double bond pigments, mainly pheophytin a, indicating that non-irrigated conditions are more stressful than irrigated ones for this species. No significant difference was found for leaf sample extracts with varying light access conditions. Keywords: Coffea arabica L. leaves, Hydric stress, Mixture design, PCA, OSC–PLS–DA |
| first_indexed | 2024-12-11T02:34:47Z |
| format | Article |
| id | doaj.art-a778ce4365e842d88d29f21637fdc145 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-11T02:34:47Z |
| publishDate | 2019-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-a778ce4365e842d88d29f21637fdc1452022-12-22T01:23:45ZengElsevierArabian Journal of Chemistry1878-53522019-12-0112842514257Environmental stress evaluation of Coffea arabica L. leaves from spectrophotometric fingerprints by PCA and OSC–PLS–DAGuilherme Luiz Scheel0Elis Daiane Pauli1Miroslava Rakocevic2Roy Edward Bruns3Ieda Spacino Scarminio4Departamento de Química, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, PR 445 Km 380, Campus Universitário, Londrina, PR CEP 86051-990, BrazilDepartamento de Química, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, PR 445 Km 380, Campus Universitário, Londrina, PR CEP 86051-990, BrazilEmbrapa Informática Agropecuária, Campus da UNICAMP, Barão Geraldo, CP6041, 13083-886 Campinas, SP, BrazilInstituto de Química, Universidade Estadual de Campinas, CP 6154, 13083-970 Campinas, SP, BrazilDepartamento de Química, Universidade Estadual de Londrina, Rod. Celso Garcia Cid, PR 445 Km 380, Campus Universitário, Londrina, PR CEP 86051-990, Brazil; Corresponding author. Tel.:+55 43 33714811; fax +55 43 33714286.The effects of hydric stress and sunlight access conditions on metabolic compounds in coffee leaves were investigated utilizing statistical mixture design extractor solvents. PCA and OSC–PLS–DA chemometric methods were used to analyze UV–visible spectra of irrigated and non-irrigated Coffea arabica L. leaves from low (<40 cm, self-shaded) and higher (>80 cm, light exposed) strata. The first latent variable of the OSC–PLS–DA score plot perfectly discriminated extracts of 34 calibration and 14 validation samples of irrigated and non-irrigated leaves. Higher spectral signals observed at the 410, 505, 535, 607 and 665 nm wavelengths are attributed to conjugate double bond pigments, mainly pheophytin a, indicating that non-irrigated conditions are more stressful than irrigated ones for this species. No significant difference was found for leaf sample extracts with varying light access conditions. Keywords: Coffea arabica L. leaves, Hydric stress, Mixture design, PCA, OSC–PLS–DAhttp://www.sciencedirect.com/science/article/pii/S1878535216300685 |
| spellingShingle | Guilherme Luiz Scheel Elis Daiane Pauli Miroslava Rakocevic Roy Edward Bruns Ieda Spacino Scarminio Environmental stress evaluation of Coffea arabica L. leaves from spectrophotometric fingerprints by PCA and OSC–PLS–DA Arabian Journal of Chemistry |
| title | Environmental stress evaluation of Coffea arabica L. leaves from spectrophotometric fingerprints by PCA and OSC–PLS–DA |
| title_full | Environmental stress evaluation of Coffea arabica L. leaves from spectrophotometric fingerprints by PCA and OSC–PLS–DA |
| title_fullStr | Environmental stress evaluation of Coffea arabica L. leaves from spectrophotometric fingerprints by PCA and OSC–PLS–DA |
| title_full_unstemmed | Environmental stress evaluation of Coffea arabica L. leaves from spectrophotometric fingerprints by PCA and OSC–PLS–DA |
| title_short | Environmental stress evaluation of Coffea arabica L. leaves from spectrophotometric fingerprints by PCA and OSC–PLS–DA |
| title_sort | environmental stress evaluation of coffea arabica l leaves from spectrophotometric fingerprints by pca and osc pls da |
| url | http://www.sciencedirect.com/science/article/pii/S1878535216300685 |
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